Study of mechanisms of repression of immune response at early stage of EHEC/EPEC infection

• Project/Area Number: 22590389
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Osaka University
• Principal Investigator: TOBE Toru 大阪大学, 大学院・医学系研究科, 教授 (70207596)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 病原性 / 病原性大腸菌 / O157 / 病原因子 / 炎症応答 / 157 / III型エフェクター / アポトーシス / EPEC / III型分泌

Research Abstract

By encountering pathogens hostcells induce inflammatory responses and activate immune responseto prevent infection of pathogens. Enterohaemorrhagic or enteropathogenic Escherichia coli (EHEC or EPEC) have ability to reduce these immune response at early stage of infection, and this ability could be important for successful infection.In this study, we identified several virulence factors involving suppression of inflammatory response and regulation of apoptosis in epithelial and immune cells.

Research Products

• [Journal Article] Activation of Motility by Sensing Short-Chain Fatty Acids via Two Steps in a Flagellar Gene Regulatory Cascade in Enterohemorrhagic Escherichia coli (2011)
  • Author(s): Tobe, T., Nakanishi, N. & Sugimoto, N
  • Journal Title: Infect. Immun Volume: 79 Issue: 3 Pages: 1016-1024
  • DOI: 10.1128/iai.00927-10
  • Peer Reviewed
• [Journal Article] Bifidobacteria can protect from enteropathogenic infection through production of acetate (2011)
  • Author(s): Fukuda S., Toh H., Hase K., Oshima K., Nakanishi Y., Yoshimura K., Tobe T
  • Journal Title: Nature Volume: 469 Pages: 543-547
  • URL: http://dx.doi.org/10.1038/nature09646
  • Peer Reviewed
• [Journal Article] Bifidobacteria can protect from enteropathogenic infection through production of acetate. (2011)
  • Author(s): Fukuda, S., et al.
  • Journal Title: Nature Volume: 469 Pages: 543-547
  • Peer Reviewed
• [Journal Article] a Type III Secretion Protease, Compromises NF-kB Activation by Targeting p65/RelA (2010)
  • Author(s): Yen, H., Ooka T., Iguchi, A., Hayashi, T., Sugimoto N., Tobe T. NleC
  • Journal Title: PLoS Pathog Volume: 6
  • URL: http://dx.doi.org/10.1371%2Fjournal.ppat.1001231
  • Peer Reviewed
• [Journal Article] Cytoskeleton-modulating effectors of enteropathogenic and enterohemorr hagic Escherichia coli : role of EspL2 in adherence and an alternative pathway for modulating cytoskeleton through Annexin A2 function. (2010)
  • Author(s): Tobe, I
  • Journal Title: FEBS J Volume: 277 Pages: 2403-2408
  • Peer Reviewed
• [Journal Article] NleC, a Type III Secretion Protease, Compromises NF-kB Activation by Targeting p65/RelA. (2010)
  • Author(s): Yen, H., et al.
  • Journal Title: PLoS Pathog Volume: 6
  • Peer Reviewed
• [Presentation] 病原性大腸菌による宿主細胞の炎症応答調節機構 (2013)
  • Author(s): 顔宏哲、戸邉亨
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉
  • Year and Date: 2013-03-19
• [Presentation] 腸管出血性大腸菌の病原性発現制御のネットワーク (2013)
  • Author(s): 戸邉亨
  • Organizer: 第155回日本獣医学会学術集会
  • Place of Presentation: 東京
  • Invited
• [Presentation] Functional analysis of EspJ as an apoptosis-enhancing effector (2012)
  • Author(s): 顔宏哲
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
  • Year and Date: 2012-03-27
• [Presentation] 腸管出血性大腸菌O157のエフェクターEspJの機能解析 (2011)
  • Author(s): 箕浦依純、顔宏哲、杉本央、戸邉亨
  • Organizer: 第64回日本細菌学会関西支部総会
  • Place of Presentation: 大阪
  • Year and Date: 2011-11-19
• [Presentation] 腸管出血性大腸菌O157のエフェクターEspJの機能解析 (2011)
  • Author(s): 箕浦依純
  • Organizer: 第64回日本細菌学会関西支部総会
  • Place of Presentation: 大阪府立大学中百舌鳥キャンパス
  • Year and Date: 2011-11-19
• [Presentation] Modulation of inflammatory response by Escherichia coli effectors (2011)
  • Author(s): 戸邉亨
  • Organizer: Singapore-Japan joint forum-Emerging concepts in Microbiology
  • Place of Presentation: Singapore(招待講演)
  • Year and Date: 2011-11-15
• [Presentation] Sensing intestinal environment for regulation of virulence expression by Enterohaemorrhagic Escherichia coli (2011)
  • Author(s): 戸邉亨
  • Organizer: IUMS2011
  • Place of Presentation: Sapporo, Japan(招待講演)
  • Year and Date: 2011-09-06
• [Presentation] a Type III Secretion Protease, Compromises NF-kB Activation by Targeting p65/RelA (2010)
  • Author(s): Yen, H., Ooka T., Iguchi, A., Hayashi, T., Sugimoto N., Tobe T. NleC
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] NleC, a Type III Secretion Protease, Compromises NF-kB Activation by Targeting p65/RelA (2010)
  • Author(s): Yen, H., et al.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10