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Development of a modified in vivo-expression technology (IVET) usingbacterial outer membrane protein as a reporter, immunoprecipitation,and microarray analysis.

Research Project

Project/Area Number 22590391
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionOsaka University

Principal Investigator

ABE Hiroyuki  大阪大学, 微生物病研究所, 助教 (00379265)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Keywords百日咳菌 / 気管支敗血症菌 / In vivoexpression technology / 宿主特異性 / IVET / 発現制御 / 感染症 / 細菌 / 宿主
Research Abstract

We developed a novel method termed IVET-IP (in vivoexpressed-tag immunoprecipitation system), which enables us to identify bacterial genes expressed in host environments. This method could be useful to search factors that determine the host specificity and pathogenicity of Bordetella spp.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (24 results)

All 2013 2012 2011 2010 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (14 results) Remarks (2 results)

  • [Journal Article] Bordetella dermonecrotic toxin binds to target cells via the N-terminal 30 amino acids2011

    • Author(s)
      Fukui-Miyazaki A, Ohnishi S, Kamitani S, Abe H, Horiguchi Y
    • Journal Title

      Microbiol Immunol.

      Volume: 55 Pages: 154-159

    • NAID

      10031121454

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystal Structure of Clostridiumperfringensenterotoxin displays features of β-pore-forming toxins2011

    • Author(s)
      Kitadokoro, K., Nishimura, K., Kamitani, S., Fukui-Miyazaki, A., Toshima, H., Abe, H., Kamata, Y., Sugita-Konishi, Y., Yamamoto, S., Karatani, H., and Horiguchi, Y
    • Journal Title

      J Biol Chem

      Volume: 286 Issue: 22 Pages: 19549-19555

    • DOI

      10.1074/jbc.m111.228478

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic Actions of Pasteurella MultocidaToxin Detected by Monoclonal Antibodies Recognizing the Deamidated Alpha Subunit of the Heterotrimeric GTPase Gq.2011

    • Author(s)
      Kamitani, S., S. Ao, H. Toshima, T. Tachibana, M. Hashimoto, K. Kitadokoro, A. Fukui-Miyazaki, H. Abe, and Y. Horiguchi
    • Journal Title

      FEBS Journal

      Volume: 278 Pages: 2702-2712

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibody recognizing the deamidated alpha subunit of the heterotrimeric GTPase Gq2011

    • Author(s)
      Kamitani, S., Ao, S., Toshima, H., Tachibana, T., Hashimoto, M., Kitadokoro, K., Fukui-Miyazaki, A., Abe, H., and Horiguchi, Y.
    • Journal Title

      FEBS J

      Volume: 278 Issue: 15 Pages: 2702-2712

    • DOI

      10.1111/j.1742-4658.2011.08197.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bordetella Dermonecrotic Toxin Binds to Target Cells Via the N-Terminal 30 Amino Acids2011

    • Author(s)
      Fukui-Miyazaki, A., S.Ohnishi, S.Kamitani, H.Abe, Y.Horiguchi
    • Journal Title

      Microbiology and Immunology

      Volume: Vol.55 Pages: 154-159

    • NAID

      10031121454

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the membrane-targeting C1 domain in Pasteurella multocidatoxin2010

    • Author(s)
      Kamitani, S., Kitadokoro, K, Miyazawa,M., Toshima, H., Fukui, A., Abe, H., Miyake,M., and Horiguchi, Y.
    • Journal Title

      J Biol Chem

      Volume: 285 Issue: 33 Pages: 25467-25475

    • DOI

      10.1074/jbc.m110.102285

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the membrane-targeting C1 domain in Pasteurella multocida toxin.2010

    • Author(s)
      Kamitani S., Abe H, et al.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 285 Pages: 25467-25475

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association of Bordetella dermonecrotic toxin with the extracellular matrix.2010

    • Author(s)
      Fukui-Miyazaki A., Abe H, et al.
    • Journal Title

      BMC Microbiology

      Volume: 10 Pages: 247-255

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] IVET-IP法: ラット感染中の気管支敗血症菌の遺伝子発現プロファイル解析2013

    • Author(s)
      安倍裕順、神谷重樹、福井理、岡田圭祐、堀口安彦
    • Organizer
      第86回 日本細菌学会総会
    • Place of Presentation
      千葉県千葉市幕張メッセ 国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] 気管支敗血症菌の新たな病原因子候補2013

    • Author(s)
      西川明芳、安倍裕順、岡田圭祐、神谷重樹、福井理、堀口安彦
    • Organizer
      第86回 日本細菌学会総会
    • Place of Presentation
      千葉県千葉市幕張メッセ 国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] 病原細菌の宿主特異性決定因子の解明2012

    • Author(s)
      安倍裕順、神谷重樹、堀口安彦、東秀明
    • Organizer
      人獣共通感染症研究拠点シンポジウム
    • Place of Presentation
      北海道札幌市北海道大学
    • Year and Date
      2012-06-14
    • Related Report
      2012 Final Research Report
  • [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発2012

    • Author(s)
      安倍裕順、神谷重樹、福井理、戸嶋ひろ野、堀口安彦
    • Organizer
      日本細菌学会総会
    • Place of Presentation
      長崎県長崎ブリックホール.
    • Year and Date
      2012-03-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発2012

    • Author(s)
      安倍裕順
    • Organizer
      日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール(長崎県)
    • Year and Date
      2012-03-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] IVET- IP法: ラット感染中の気管支敗血症菌の遺伝子発現プロファイル解析.2012

    • Author(s)
      安倍裕順、神谷重樹、福井理、岡田圭祐、堀口安彦
    • Organizer
      日本細菌学会総会
    • Place of Presentation
      千葉県千葉市幕張メッセ国際会議場.
    • Year and Date
      2012-03-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 気管支敗血症菌の新たな病原因子候補.2012

    • Author(s)
      西川明芳、安倍裕順、岡田圭祐、神谷重樹、福井 理、堀口安彦
    • Organizer
      日本細菌学会総会.
    • Place of Presentation
      千葉県千葉市幕張メッセ国際会議場
    • Year and Date
      2012-03-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発2011

    • Author(s)
      安倍裕順、神谷重樹、福井理、戸嶋ひろ野、堀口安彦
    • Place of Presentation
      神奈川県パシフィコ横浜
    • Year and Date
      2011-12-14
    • Related Report
      2012 Final Research Report
  • [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発2011

    • Author(s)
      安倍裕順
    • Organizer
      日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発2011

    • Author(s)
      安倍裕順、神谷重樹、福井理、戸嶋ひろ野、堀口安彦
    • Organizer
      細菌学会関西支部総会
    • Place of Presentation
      大阪府大阪府立大学中百舌鳥キャンパス学術交流会館多目的ホール.
    • Year and Date
      2011-11-19
    • Related Report
      2012 Final Research Report
  • [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発2011

    • Author(s)
      安倍裕順
    • Organizer
      細菌学会関西支部総会
    • Place of Presentation
      大阪府立大学中百舌鳥キャンパス学術交流会館多目的ホール(大阪府)
    • Year and Date
      2011-11-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発2011

    • Author(s)
      安倍裕順
    • Organizer
      細菌学若手コロッセウム
    • Place of Presentation
      高知県国民宿舎桂浜荘.
    • Year and Date
      2011-08-09
    • Related Report
      2012 Final Research Report
  • [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発2011

    • Author(s)
      安倍裕順
    • Organizer
      細菌学若手コロッセウム
    • Place of Presentation
      国民宿舎桂浜荘(高知県)
    • Year and Date
      2011-08-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] 病原細菌の宿主特異性決定因子の解明

    • Author(s)
      安倍裕順、神谷重樹、堀口安彦、東秀明
    • Organizer
      第1回 人獣共通感染症研究拠点シンポジウム
    • Place of Presentation
      北海道札幌市北海道大学
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://bactox1.biken.osaka-u.ac.jp

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://bactox1.biken.osaka-u.ac.jp/2011Web/Horiguchis_Lab_top.html

    • Related Report
      2011 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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