Development of a modified in vivo-expression technology (IVET) usingbacterial outer membrane protein as a reporter, immunoprecipitation,and microarray analysis.
Project/Area Number |
22590391
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Bacteriology (including Mycology)
|
Research Institution | Osaka University |
Principal Investigator |
ABE Hiroyuki 大阪大学, 微生物病研究所, 助教 (00379265)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
|
Keywords | 百日咳菌 / 気管支敗血症菌 / In vivoexpression technology / 宿主特異性 / IVET / 発現制御 / 感染症 / 細菌 / 宿主 |
Research Abstract |
We developed a novel method termed IVET-IP (in vivoexpressed-tag immunoprecipitation system), which enables us to identify bacterial genes expressed in host environments. This method could be useful to search factors that determine the host specificity and pathogenicity of Bordetella spp.
|
Report
(4 results)
Research Products
(24 results)
-
-
[Journal Article] Crystal Structure of Clostridiumperfringensenterotoxin displays features of β-pore-forming toxins2011
Author(s)
Kitadokoro, K., Nishimura, K., Kamitani, S., Fukui-Miyazaki, A., Toshima, H., Abe, H., Kamata, Y., Sugita-Konishi, Y., Yamamoto, S., Karatani, H., and Horiguchi, Y
-
Journal Title
J Biol Chem
Volume: 286
Issue: 22
Pages: 19549-19555
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibody recognizing the deamidated alpha subunit of the heterotrimeric GTPase Gq2011
Author(s)
Kamitani, S., Ao, S., Toshima, H., Tachibana, T., Hashimoto, M., Kitadokoro, K., Fukui-Miyazaki, A., Abe, H., and Horiguchi, Y.
-
Journal Title
FEBS J
Volume: 278
Issue: 15
Pages: 2702-2712
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Characterization of the membrane-targeting C1 domain in Pasteurella multocidatoxin2010
Author(s)
Kamitani, S., Kitadokoro, K, Miyazawa,M., Toshima, H., Fukui, A., Abe, H., Miyake,M., and Horiguchi, Y.
-
Journal Title
J Biol Chem
Volume: 285
Issue: 33
Pages: 25467-25475
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-