Development of a modified in vivo-expression technology (IVET) usingbacterial outer membrane protein as a reporter, immunoprecipitation,and microarray analysis.

• Project/Area Number: 22590391
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Osaka University
• Principal Investigator: ABE Hiroyuki 大阪大学, 微生物病研究所, 助教 (00379265)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 百日咳菌 / 気管支敗血症菌 / In vivoexpression technology / 宿主特異性 / IVET / 発現制御 / 感染症 / 細菌 / 宿主

Research Abstract

We developed a novel method termed IVET-IP (in vivoexpressed-tag immunoprecipitation system), which enables us to identify bacterial genes expressed in host environments. This method could be useful to search factors that determine the host specificity and pathogenicity of Bordetella spp.

Research Products

• [Journal Article] Bordetella dermonecrotic toxin binds to target cells via the N-terminal 30 amino acids (2011)
  • Author(s): Fukui-Miyazaki A, Ohnishi S, Kamitani S, Abe H, Horiguchi Y
  • Journal Title: Microbiol Immunol. Volume: 55 Pages: 154-159
  • NAID: 10031121454
  • Peer Reviewed
• [Journal Article] Crystal Structure of Clostridiumperfringensenterotoxin displays features of β-pore-forming toxins (2011)
  • Author(s): Kitadokoro, K., Nishimura, K., Kamitani, S., Fukui-Miyazaki, A., Toshima, H., Abe, H., Kamata, Y., Sugita-Konishi, Y., Yamamoto, S., Karatani, H., and Horiguchi, Y
  • Journal Title: J Biol Chem Volume: 286 Issue: 22 Pages: 19549-19555
  • DOI: 10.1074/jbc.m111.228478
  • Peer Reviewed
• [Journal Article] Enzymatic Actions of Pasteurella MultocidaToxin Detected by Monoclonal Antibodies Recognizing the Deamidated Alpha Subunit of the Heterotrimeric GTPase Gq. (2011)
  • Author(s): Kamitani, S., S. Ao, H. Toshima, T. Tachibana, M. Hashimoto, K. Kitadokoro, A. Fukui-Miyazaki, H. Abe, and Y. Horiguchi
  • Journal Title: FEBS Journal Volume: 278 Pages: 2702-2712
  • Peer Reviewed
• [Journal Article] Enzymatic actions of Pasteurella multocida toxin detected by monoclonal antibody recognizing the deamidated alpha subunit of the heterotrimeric GTPase Gq (2011)
  • Author(s): Kamitani, S., Ao, S., Toshima, H., Tachibana, T., Hashimoto, M., Kitadokoro, K., Fukui-Miyazaki, A., Abe, H., and Horiguchi, Y.
  • Journal Title: FEBS J Volume: 278 Issue: 15 Pages: 2702-2712
  • DOI: 10.1111/j.1742-4658.2011.08197.x
  • Peer Reviewed
• [Journal Article] Bordetella Dermonecrotic Toxin Binds to Target Cells Via the N-Terminal 30 Amino Acids (2011)
  • Author(s): Fukui-Miyazaki, A., S.Ohnishi, S.Kamitani, H.Abe, Y.Horiguchi
  • Journal Title: Microbiology and Immunology Volume: Vol.55 Pages: 154-159
  • NAID: 10031121454
  • Peer Reviewed
• [Journal Article] Characterization of the membrane-targeting C1 domain in Pasteurella multocidatoxin (2010)
  • Author(s): Kamitani, S., Kitadokoro, K, Miyazawa,M., Toshima, H., Fukui, A., Abe, H., Miyake,M., and Horiguchi, Y.
  • Journal Title: J Biol Chem Volume: 285 Issue: 33 Pages: 25467-25475
  • DOI: 10.1074/jbc.m110.102285
  • Peer Reviewed
• [Journal Article] Characterization of the membrane-targeting C1 domain in Pasteurella multocida toxin. (2010)
  • Author(s): Kamitani S., Abe H, et al.
  • Journal Title: The Journal of Biological Chemistry Volume: 285 Pages: 25467-25475
  • Peer Reviewed
• [Journal Article] Association of Bordetella dermonecrotic toxin with the extracellular matrix. (2010)
  • Author(s): Fukui-Miyazaki A., Abe H, et al.
  • Journal Title: BMC Microbiology Volume: 10 Pages: 247-255
  • Peer Reviewed
• [Presentation] IVET-IP法: ラット感染中の気管支敗血症菌の遺伝子発現プロファイル解析 (2013)
  • Author(s): 安倍裕順、神谷重樹、福井理、岡田圭祐、堀口安彦
  • Organizer: 第86回 日本細菌学会総会
  • Place of Presentation: 千葉県千葉市幕張メッセ 国際会議場
• [Presentation] 気管支敗血症菌の新たな病原因子候補 (2013)
  • Author(s): 西川明芳、安倍裕順、岡田圭祐、神谷重樹、福井理、堀口安彦
  • Organizer: 第86回 日本細菌学会総会
  • Place of Presentation: 千葉県千葉市幕張メッセ 国際会議場
• [Presentation] 病原細菌の宿主特異性決定因子の解明 (2012)
  • Author(s): 安倍裕順、神谷重樹、堀口安彦、東秀明
  • Organizer: 人獣共通感染症研究拠点シンポジウム
  • Place of Presentation: 北海道札幌市北海道大学
  • Year and Date: 2012-06-14
• [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発 (2012)
  • Author(s): 安倍裕順、神谷重樹、福井理、戸嶋ひろ野、堀口安彦
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 長崎県長崎ブリックホール.
  • Year and Date: 2012-03-27
• [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発 (2012)
  • Author(s): 安倍裕順
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール(長崎県)
  • Year and Date: 2012-03-27
• [Presentation] IVET- IP法: ラット感染中の気管支敗血症菌の遺伝子発現プロファイル解析. (2012)
  • Author(s): 安倍裕順、神谷重樹、福井理、岡田圭祐、堀口安彦
  • Organizer: 日本細菌学会総会
  • Place of Presentation: 千葉県千葉市幕張メッセ国際会議場.
  • Year and Date: 2012-03-20
• [Presentation] 気管支敗血症菌の新たな病原因子候補. (2012)
  • Author(s): 西川明芳、安倍裕順、岡田圭祐、神谷重樹、福井 理、堀口安彦
  • Organizer: 日本細菌学会総会.
  • Place of Presentation: 千葉県千葉市幕張メッセ国際会議場
  • Year and Date: 2012-03-20
• [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発 (2011)
  • Author(s): 安倍裕順、神谷重樹、福井理、戸嶋ひろ野、堀口安彦
  • Place of Presentation: 神奈川県パシフィコ横浜
  • Year and Date: 2011-12-14
• [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発 (2011)
  • Author(s): 安倍裕順
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-14
• [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発 (2011)
  • Author(s): 安倍裕順、神谷重樹、福井理、戸嶋ひろ野、堀口安彦
  • Organizer: 細菌学会関西支部総会
  • Place of Presentation: 大阪府大阪府立大学中百舌鳥キャンパス学術交流会館多目的ホール.
  • Year and Date: 2011-11-19
• [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発 (2011)
  • Author(s): 安倍裕順
  • Organizer: 細菌学会関西支部総会
  • Place of Presentation: 大阪府立大学中百舌鳥キャンパス学術交流会館多目的ホール(大阪府)
  • Year and Date: 2011-11-19
• [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発 (2011)
  • Author(s): 安倍裕順
  • Organizer: 細菌学若手コロッセウム
  • Place of Presentation: 高知県国民宿舎桂浜荘.
  • Year and Date: 2011-08-09
• [Presentation] 感染成立過程で発現する病原細菌遺伝子の網羅的解析法の開発 (2011)
  • Author(s): 安倍裕順
  • Organizer: 細菌学若手コロッセウム
  • Place of Presentation: 国民宿舎桂浜荘(高知県)
  • Year and Date: 2011-08-09
• [Presentation] 病原細菌の宿主特異性決定因子の解明
  • Author(s): 安倍裕順、神谷重樹、堀口安彦、東秀明
  • Organizer: 第1回 人獣共通感染症研究拠点シンポジウム
  • Place of Presentation: 北海道札幌市北海道大学
• [Remarks]
  • URL: http://bactox1.biken.osaka-u.ac.jp
• [Remarks]
  • URL: http://bactox1.biken.osaka-u.ac.jp/2011Web/Horiguchis_Lab_top.html