The approach of synthetic steroid ceragenin to curative medicine for infectious diseases

• Project/Area Number: 22590397
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Sapporo Medical University
• Principal Investigator: ISOGAI Hiroshi 札幌医科大学, 医学部, 准教授 (50137436)
• Co-Investigator(Kenkyū-buntansha): ISOGAI Emiko 東北大学, 大学院農学研究科, 教授 (80113570)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 感染症 / 微生物 / 抗菌 / CSA / Ceragenin / 抗菌ステロイド / 抗菌タンパク / 感染防御 / ＣＳＡ

Research Abstract

Ceragenin (CSA) is the antibacterial material which has a steroid ring. CSA covered and mixed to a dental material. It controlled to bacterial biofilm formation on those surfaces and inhibited the bacterial growth in liquid by exuding in culture solution. Moreover, a bacterial resistant was not induced, even if staphylococcus was cultivated repeatedly in low-concentration of CSA. CSA showed the antitumor activity by inducing apoptosis to cancer cells.

Research Products

• [Journal Article] Ceragenin CSA-13 induces cell cycle arrest and antiproliferative effects in wild-type and p53 null mutant HCT116 colon cancer cells (2013)
  • Author(s): Kuroda K., Fukuda T., Okumura K., Yoneyama H., Isogai H., Savage P.B., Isogai E.
  • Journal Title: Anti Cancer Drags Volume: 23 Issue: 8 Pages: 826-834
  • DOI: 10.1097/cad.0b013e3283634dd0
  • Peer Reviewed
• [Journal Article] Detection of virulence genes of Escherichia coli and Salmonella spp. from fecal samples of Kafue lechwe (Kobus leche kafuensis) and pastoral cattle in the interface areas of Zambia (2013)
  • Author(s): Kuroda K., Suzuki R., Ihara K., Miyagi H., Watanabe H., Sato K., Mudenda B. H., Mubita C., Isogai N., Mulenga E., Moonga L., Isogai H., Fukuda T., Yoneyama H., and Isogai E.
  • Journal Title: African Journal of Microbiology Research Volume: Vol. 7(6) Pages: 504-508
  • Peer Reviewed
• [Journal Article] Detection of virulence genes of Escherichia coli and Salmonella spp. from fecal samples of Kafue lechwe (Kobus leche kafuensis) and pastoral cattle in the interface areas of Zambia (2013)
  • Author(s): Kengo Kuroda
  • Journal Title: African Journal of Microbiology Research Volume: 7 Pages: 504-508
  • Peer Reviewed
• [Journal Article] Anti-proliferative effect of the analogue peptide of human Cathelicidin (hCAP18/LL-37) for the colon cancer derived cell line, HCT116 p53+/+ and p53-/- (2012)
  • Author(s): Kuroda K, Fukuda T, Yoneyama H, Katayama M, Isogai H, Okumura K, and Isogai E
  • Journal Title: Oncology Reports Volume: 28 Issue: 3 Pages: 829-834
  • DOI: 10.3892/or.2012.1876
  • Peer Reviewed
• [Journal Article] Antimicrobial activity of a bovine myeloid antimicrobial peptide (BMAP-28) against methicillin-susceptible and methicillin-resistant Staphylococcus aureus (2012)
  • Author(s): Takagi S, Hayashi S, Takahashi K, Isogai H, Bai L, Yoneyama H, Ando T, Ito K, Isogai E
  • Journal Title: Anim Sci J Volume: 83 Issue: 6 Pages: 482-486
  • DOI: 10.1111/j.1740-0929.2011.00979.x
  • Peer Reviewed
• [Journal Article] Anti-proliferative effect of the analogue peptide of human Cathelicidin (hCAP18/LL-37) for the colon cancer derived cell line, HCT116 p53+/+ and p53-/- (2012)
  • Author(s): Kuroda K, Fukuda T, Yoneyama H, Katayama M, Isogai H, Okumura K, Isogai E
  • Journal Title: Oncology Reports Volume: (In press)
  • Peer Reviewed
• [Journal Article] High expression of MeCP2 in JC virus-infected cells of progressive multifocal leukoencephalopathy brains (2011)
  • Author(s): Shirai S, Takahashi K, Kohsaka S, Tsukamoto T, Isogai H, Kudo S, Sawa H, Nagashima K, Tanaka S
  • Journal Title: Neuropathology Volume: 31 Issue: 1 Pages: 38-41
  • DOI: 10.1111/j.1440-1789.2010.01122.x
  • NAID: 120005893285
  • Peer Reviewed
• [Journal Article] 口腔におけるカセリシジンファミリ-抗菌ペプチドCAP18/LL37の微生物制御 (2010)
  • Author(s): 磯貝浩、磯貝恵美子、奥村一彦、南場研一、北市伸義、大野重昭、角館直樹
  • Journal Title: J.germfree life gnotobiol. Volume: 40 Pages: 8-13
  • NAID: 10027130351
  • Peer Reviewed
• [Journal Article] 消化管由来の病原および非病原細菌を摂接種されたマウスにおける単一細菌の定着と炎症性サイトカインおよび末梢血細胞数 (2010)
  • Author(s): 磯貝恵美子、磯貝浩、小林美智代、奥村一彦
  • Journal Title: 無菌生物 Volume: 40 Pages: 50-53
  • Peer Reviewed
• [Presentation] Bactericidal ability of recombinant peptide of cationic antimicrobial peptides (2013)
  • Author(s): Isogai, H., Isogai, E., Kuroda K.
  • Organizer: 18th World Congress on Advances in Oncology and 16th International Symposium on Molecular Medicine
  • Place of Presentation: イラクリオン、ギリシャ
• [Presentation] Characteristics of bovine myeloid antimicrobial peptide (BMAP-28) as active antimicrobials (2013)
  • Author(s): Takagi S., Isogai E., Nishimura J., Bai L., Kuroda K., Suzuki R., Yoneyama H., Hayashi S., Isogai H.
  • Organizer: 18th World Congress on Advances in Oncology and 16th International Symposium on Molecular Medicine
  • Place of Presentation: イラクリオン、ギリシャ
• [Presentation] Bactericidal ability of recombinant peptide of cationic antimicrobial peptides (2013)
  • Author(s): Hiroshi ISOGAI, Emiko ISOGAI, Kengo KURODA
  • Organizer: 18th World Congress on Advances in Oncology and 16th International Symposium on Molecular Medicine
  • Place of Presentation: ギリシャ イラクリオン
• [Presentation] Characteristics of bovine myeloid antimicrobial peptide (BMAP-28) as active antimicrobials. (2013)
  • Author(s): S. Takagi, E. Isogai, J. Nishimura, L. Bai, K. Kuroda, R. Suzuki, H. Yoneyama, S. Hayashi, H. Isogai
  • Organizer: 18th World Congress on Advances in Oncology and 16th International Symposium on Molecular Medicine
  • Place of Presentation: ギリシャ イラクリオン
• [Presentation] Suppressive effects of cationic antimicrobial peptides and mimics on colon cancer cell line HCT116 p53+/+ and p53-/-. (2013)
  • Author(s): K. Kuroda, T. Fukuda, H. Yoneyama, K. Okumura, H. Isogai, P.B. Savage, E. Isogai
  • Organizer: 18th World Congress on Advances in Oncology and 16th International Symposium on Molecular Medicine
  • Place of Presentation: ギリシャ イラクリオン
• [Presentation] Correlation between levels of antibody against Lyme disease Borrelia and tick defensin in wild deer (2012)
  • Author(s): Isogai H, Isogai E
  • Organizer: 3rd International Symposium on Antimicrobial Peptides (Amp2012)
  • Place of Presentation: Lille, France
• [Presentation] Analogue peptide of LL-37 induces anti-proliferative effect for colon cancer derived cell line (2012)
  • Author(s): Kuroda K, Fukuda T, Yoneyama H, Katayama M, Isogai H, Okumura K, Isogai E
  • Organizer: 3rd International Symposium on Antimicrobial Peptides (Amp2012)
  • Place of Presentation: Lille, France
• [Presentation] Serume and salivery levels of bioactive antimicrobial peptide LL-37 in Behcet's disease and other ocular inflammatory diseases (2012)
  • Author(s): Isogai E, Kuroda K, Seki S, Kitaichi N, Namba K, Ohno S, Yoneyama H, Isogai H
  • Organizer: 3rd International Symposium on Antimicrobial Peptides (Amp2012)
  • Place of Presentation: Lille, France
• [Presentation] Serume and salivery levels of bioactive antimicrobial peptide LL-37 in Behcet’s disease and other ocular inflammatory diseases (2012)
  • Author(s): Isogai E
  • Organizer: 3rd International Symposium on Antimicrobial Peptides (Amp2012)
  • Place of Presentation: Lille, France
• [Presentation] Analogue peptide of LL-37 induces anti-proliferative effect for colon cancer derived cell line, HCT116 p53+/+ and p53-/- (2012)
  • Author(s): Kuroda K
  • Organizer: 3rd International Symposium on Antimicrobial Peptides (Amp2012)
  • Place of Presentation: Lille, France
• [Presentation] Kurebayashi Y Antimicrobial activity of a bovine myeloid antimicrobial pepetide (BMAP-28) against methicillin- susceptible and methicillin-resistant Staphylococcus aureus and prediction of the structure of BMAP-28 by homology modeling (2011)
  • Author(s): Isogai E, Saito T, Omura T, Isogai H, Okumura K, Hori H, Tsuruta H
  • Organizer: Internatioal Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-06
• [Presentation] Antimicrobial activity of a bovine myeloid antimicrobial pepetide (BMAP-28) against methicillin- susceptible and methicillin-resistant Staphylococcus aureus and prediction of the structure of BMAP-28 by homology modeling (2011)
  • Author(s): Takagi S, Saito T, Ohmura T, Isogai H, Yoneyama H, Ando T, Bai L, Fukuda T, Kato Y, Nakai Y, Isogai E
  • Organizer: Internatioal Union of Microbiological Societies 2011 Congress
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-06
• [Presentation] Cathelicidin Antimicrobial Peptide, LL-37 has Proangiogenic and Angiostatic Activity in Human Microvascular endothelial Cells (2011)
  • Author(s): Okumura K, Taira H, Kobayashi M, Isogai E, Shibata T, Isogai H
  • Organizer: Internatioal Union of Microbiological Societies, 2011 Congress
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-06
• [Presentation] Cathelicidin Antimicrobial Peptide, LL-37 has Proangiogenic and Angiostatic Activity in Human Microvascular endothelial Cells (2011)
  • Author(s): Okumura K, Taira H, Kobayashi M, Isogai E, Shibata T, Isogai H
  • Organizer: Internatioal Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
  • Year and Date: 2011-09-06
• [Presentation] Antimicrobial activity of a bovine myeloid antimicrobial pepetide (BMAP-28)against methicillin-susceptible and methicillin-resistant Staphylococcus aureus and prediction of the structure of BMAP-28 by homology modeling (2011)
  • Author(s): Takagi S, Saito T, Ohmura T, Isogai H, Yoneyama H, Ando T, Bai L, Fukuda T, Kato Y, Nakai Y, Isogai E
  • Organizer: Internatioal Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
  • Year and Date: 2011-09-06
• [Presentation] Homology modeling and tertiary structure-related activity of tick deffensin (persulcatusin) in the taiga tick, Ixodes persulcatus (2011)
  • Author(s): Isogai E, Saito T, Omura T, Isogai H, Okumura K, Hori H, Tsuruta H, Kurebayashi Y
  • Organizer: Intematioal Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo
  • Year and Date: 2011-09-06
• [Remarks] 磯貝恵美子、磯貝浩、小林美智代、奥村一彦 消化管由来の病原および非病原細菌を摂接種されたマウスにおける単一細菌の定着と炎症性サイトカインおよび末梢血細胞数無菌生物(2010) 40: 50-53
• [Remarks] 磯貝浩、磯貝恵美子、奥村一彦、南場研一、北市伸義、大野重昭、角館直樹 口腔におけるカセリシジンファミリ-抗菌ペプチドCAP18/LL37の微生物制御無菌生物(2010) 40: 54-59