| Project/Area Number |
22590400
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Okayama Prefectural University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAKATA Kazue 岡山県立大学, 保健福祉学部, 助教 (60411740)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ビブリオ / バルニフィカス菌 / 感染 / 毒素 / トランスポゾン / ワクチン / バルニフィカス / 肝疾患 / 魚介類 / Vibrio vulnificus / 食品衛生 / 細菌 / 遺伝子 / 肝硬変 / 病原因子 / 食品の安全 / 海洋細菌 |
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| Research Abstract |
Vibrio vulnificus is an important pathogens of foodborn diseases and wound infection. This pathogen causes fatal septicemia rapidly in immunocompromised hosts after they contact with contaminated seawater or seafood with this organism. The mortality rate is more than 50%. For the rapid progress and the high mortality,vaccination is considered an effective way to control infection with V. vulnificus in a high risk population. We applied Signature-Tagged Mutagenesis (STM) to generate attenuated mutants from a clinically-isolated virulent V. vulnificus strain, OPU1 which was isolated from a patient. Twelve insertion mutants were selected and the virulence of these mutants were determined by the minimal lethal dose (MLD) to mice. Several mutant strains showed lower virulence compare to the parent virulent strain. The DNA sequences flanking to transposon insertion sites in these mutants were determined and searched for homologies at DNA Date Bank of Japan. The transposons inserted genes were those for IMP dehydrogenase, UDP-N-acetylgulcosamin-2-epimerase, aspartokinase, and a conserved hypothetical protein. This suggests that these genes may be involved in V. vulnificusvirulence. One of the mutants was administrated for experimental vaccination to mice. The immunized mice were challenged with the virulent parent strain. All unimmunized mice were killed within 36 hr, but almost all immunized mice survived. In this experiment, we found that the attenuated have a potentiality as live vaccine candidate.
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