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Integrated analyses of virulence factors from Staphylococcus aureusaiming to pursue novel pathogenic mechanisms and chemotherapy based on new concept

Research Project

Project/Area Number 22590402
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionJuntendo University

Principal Investigator

BABA Tadashi  順天堂大学, 医学部, 准教授 (30317458)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords黄色ブドウ球菌 / 赤血球溶解毒素 / β-ヘモリジン / ヒトケラチノサイト / アトピー性皮膚炎 / nybomycin / キノロン / gyrase / ヒト皮膚角化細胞 / バクテリオファージ / 病原性 / ケラチノサイト / 病原性アイランド / 細胞毒性 / 免疫細胞 / 宿主特異性 / MHC / TSLP / 好中球
Research Abstract

On the way to pursue novel pathogenic mechanisms that can be candidates for targets of novel chemotherapy, I found ・-hemolysin, known as an erythrolytic toxin from Staphylococcus aureus, damages human keratinocytes. This result suggests that the toxin is presumably involved in atopic dermatitis that is worsened by S. aureus infection, and the finding may lead to further understanding of the disease and its therapy. I also played part in research on re-evaluation of nybomycin, that had been isolated from actinobacteria over 50 years ago, and showed that the substance specifically inhibits function of mutated bacterial DNA gyrase that was no longer inactivated by fluoroquinolones, whereas nybomycin-resistant DNA gyrase acquired fluoroquinolone susceptibility, indicating fluoroquinolones and nybomicin have mutually complementary functions. Such substance against known antibiotic was designated ‘reverse antibiotic’, being expected to open new era of antibiotic chemotherapy. It should be noted that the results described above were obtained cooperative researches with scientists who were originally out of this project.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (22 results)

All 2013 2012 2011 2010

All Journal Article (12 results) (of which Peer Reviewed: 6 results) Presentation (8 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 2 results)

  • [Journal Article] Beta-hemolysin promotes skin colonization of Staphylococcus aureus2013

    • Author(s)
      Katayama Y, Baba T, Sekine M, Fukuda M, and Hiramatsu K
    • Journal Title

      J Bacteriol

      Volume: 195 Pages: 1194-1203

    • Related Report
      2012 Final Research Report
  • [Journal Article] Beta-hemolysin promotes skin colonization by Staphylococcus aureus.2013

    • Author(s)
      Katayama Y, Baba T, Sekine M, Fukuda M, Hiramatsu K.
    • Journal Title

      Journal of Bacteriology

      Volume: 195 Issue: 6 Pages: 1194-1203

    • DOI

      10.1128/jb.01786-12

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Curing bacteria of antibiotic resistance: reverse antibiotics, a novel class of antibiotics in nature2012

    • Author(s)
      Hiramatsu K, Igarashi M, Morimoto Y, Baba T, Umekita M, Akamatsu Y
    • Journal Title

      Int J Antimicrob Agents

      Volume: 39 Pages: 478-85

    • Related Report
      2012 Final Research Report
  • [Journal Article] Expression and Functional Characterization of Retinoic Acid-Inducible Gene-I-Like Receptors of Mast Cells in Response to Viral Infection2012

    • Author(s)
      Fukuda M, Ushio H, Kawasaki J, Niyonsaba F, Takeuchi M, Baba T, Hiramatsu K, Okumura K, Ogawa H
    • Journal Title

      J Innate Immun

      Volume: (Epub ahead of print)

    • Related Report
      2012 Final Research Report
  • [Journal Article] Expression and Functional Characterization of Retinoic Acid-Inducible Gene-I-Like Receptors of Mast Cells in Response to Viral Infection.2012

    • Author(s)
      Fukuda M, Ushio H, Kawasaki J, Niyonsaba F, Takeuchi M, Baba T, Hiramatsu K, Okumura K, Ogawa H.
    • Journal Title

      Journal of Innate Immunity

      Volume: 5 Issue: 2 Pages: 163-173

    • DOI

      10.1159/000343895

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence2011

    • Author(s)
      Kaito C, Saito Y, Nagano G, Ikuo M, Omae Y, Hanada Y, Han X, Kuwahara-Arai K, Hishinuma T, Baba T, Ito T, Hiramatsu K, Sekimizu K
    • Journal Title

      PLos Pathg

      Volume: 7

    • Related Report
      2012 Final Research Report
  • [Journal Article] 黄色ブドウ球菌 MHC 様分子が貪食などの宿主免疫系に及ぼす影響について2011

    • Author(s)
      関根 美和・馬場 理・片山 由紀・平松啓一
    • Journal Title

      順天堂医学

      Volume: 57(3)

    • NAID

      10029499381

    • Related Report
      2012 Final Research Report
  • [Journal Article] Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence2011

    • Author(s)
      Kaito C, et al
    • Journal Title

      PLos Pathogens

      Volume: 7

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 黄色ブドウ球菌MHC様分子が貪食などの宿主免疫系に及ぼす影響について(MHC analogues found in Staphylococcus aureus affect phagocytosis and other immune responses by human whit e-blood cells)2011

    • Author(s)
      関根美和, 他
    • Journal Title

      順天堂医学

      Volume: 57 Pages: 232-242

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 黄色ブドウ球菌MHC様分子が貪食などの宿主免疫系に及ぼす影響について(MHC analogues found in Staphylococcus aureus affect phagocytosis and other immune responses by human white-blood cells)2011

    • Author(s)
      関根美和・馬場理・片山由
    • Journal Title

      順天堂医学

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Staphylococcus aureus membrane and diacylated lipopeptide induce thymic stromal lymphopoietin in keratinocytes through the Toll-like receptor 2-Toll-like receptor 6 pathway2010

    • Author(s)
      Anh Tuan Vu, Tadashi Baba, Xue Chen, Tuan Anh Le, Hirokazu Kinoshita, Yang Xie, Seiji Kamijo, Keiichi Hiramatsu, Shigaku Ikeda, Hideoki Ogawa, Ko Okumura, Toshiro Takai
    • Journal Title

      J. allergy clin immunol

      Volume: 126 Pages: 985-93

    • Related Report
      2012 Final Research Report
  • [Journal Article] Staphylococcus aureus membrane and diacylated lipopeptide induce thymic stromal lymphopoietin in keratinocytes through the Toll-like receptor 2-Toll-like receptor 6 pathway.2010

    • Author(s)
      Anh Tuan Vu, Tadashi Baba, Xue Chen, Tuan Anh Le, Hirokazu Kinoshita, Yang Xie, Seiji Kamijo, Keiichi Hiramatsu, Shigaku Ikeda, Hideoki Ogawa, Ko Okumura, Toshiro Takai.
    • Journal Title

      J.allergy clin immunol

      Volume: 126 Pages: 985-93

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Combined Use of Fluoroquinolone with Nybomycin, a Novel Category of Antibiotics “Reverse Antibiotic”2013

    • Author(s)
      Y Morimoto, M Igarashi, T Baba, M Umekita, Y Akamatsu, K Hiramatsu.
    • Organizer
      International Symposium on Antimicrobial Agents and Resistance 2013
    • Place of Presentation
      Kuala Lumpur Convention Centre, Malaysia
    • Related Report
      2012 Annual Research Report
  • [Presentation] Antimicrobial activity of Nybomycin against Escherichia coli2013

    • Author(s)
      Y. Morimoto, M. Igarashi, T. Baba, K. Hiramatsu
    • Organizer
      The 28th International Congress of Chemotherapy
    • Place of Presentation
      Pacifico Yokohama, Kanagawa Japan.
    • Related Report
      2012 Annual Research Report
  • [Presentation] Two specific genomic islands found in S.aureus and their involvement in pathogenesis2012

    • Author(s)
      T.Baba, M.Sekine, M.Fukuda, K.Hiramatsu
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] MHC analogues found in S.aureus affect phagocytosis and other immune responses2012

    • Author(s)
      M.Sekine, T.Baba, Y.Katayama, K.Hiramatsu
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] Pathogenicity of S.aureus VISA strains upon various cultured primary human tissue cells2012

    • Author(s)
      M.Fukuda, T.Baba, K.Hiramatsu
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] Streptococcus intermediusゲノムにみられるmicrocompartment遺伝子群と嫌気好性/炭酸ガス要求性との関連2012

    • Author(s)
      K.Kikuchi, M.Matsuda, T.Baba, L.Cui, K.Hiramatsu
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎ブリックホール
    • Year and Date
      2012-03-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Genomic islands in Staphylococcus aureus and their involvement in pathogenesis2011

    • Author(s)
      T.Baba, M.Sekine, Y.Katayama, M.Fukuda, K.Hiramatsu
    • Organizer
      International Union of Microbiological Societies Congresses 2011
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation]2010

    • Organizer
      日本細菌学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2010-03-27
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 新規化合物、DNAジャイレース阻害剤及びその用途2013

    • Inventor(s)
      平松啓一(順天堂大学)森本ゆふ(順天堂大学) 馬場 理 (順天堂大学)早川勇夫(順天堂大学
    • Industrial Property Rights Holder
      順天堂大学
    • Industrial Property Number
      2013-088008
    • Filing Date
      2013
    • Related Report
      2012 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 毒素検出方法2012

    • Inventor(s)
      大石金成(デンカ生研) 馬場 理 (順天堂大学)平松啓一(順天堂大学)中臣康雄(デンカ生研)
    • Industrial Property Rights Holder
      デンカ生研・順天堂大学
    • Patent Publication Number
      2013-053154
    • Filing Date
      2012-12-04
    • Related Report
      2012 Final Research Report
    • Overseas

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Published: 2010-08-23   Modified: 2019-07-29  

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