Identification of the botulinum toxin receptor protein on the intestinal epithelial cells
| Project/Area Number |
22590405
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Tokyo University of Agriculture |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OOYAMA Toru 東京農業大学, 生物産業学部, 教授 (60318178)
NIWA Koichi 東京農業大学, 生物産業学部, 教授 (20301012)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 細菌学(含真菌学) / ボツリヌス毒素複合体 / 小腸上皮細胞 / ボツリヌス食中毒 / 受容体 / 糖鎖 / シアル酸 |
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| Research Abstract |
Botulinum toxin complex (TC) is composed of neurotoxin, NTNHA,HA-70, HA-33, and HA-17. TC enters into blood stream via small intestinal epithelium. In this study, we revealed that the toxin complex binds to the different sugar-chain depending on the struc
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Report
(4 results)
Research Products
(44 results)
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[Journal Article] Random phage display based screening of peptides that bind to botulinum neurotoxin binding protein, nontoxic nonhemagglutinin.2013
Author(s)
Sagane Y, Miyata K, Kurihara S, Yoneyama T, Inui K, Miyashita S, Hayashi S, Suzuki T, Niwa K,
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Journal Title
Current Microbiology
Volume: (Online)
Issue: 2
Pages: 188-192
DOI
Related Report
Peer Reviewed
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[Journal Article] Small-angle X-ray scattering reveals structural dynamics of the botulinum neurotoxin associating protein, nontoxic nonhemagglutinin.2012
Author(s)
Sagane Y, Miyashita S, Miyata K, Matsumoto T, Inui K, Hayashi S, Suzuki T, Hasegawa K, Yajima S, Yamano A, Niwa K, Watanabe T.
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 425
Issue: 2
Pages: 256-260
DOI
Related Report
Peer Reviewed
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[Journal Article] Crystallization and preliminary X-ray analysis of the Clostridium botulinum type D nontoxic nonhaemagglutinin2012
Author(s)
Miyata, K., Inui, K., Miyashita, S., Sagane, Y., Hasegawa, K., Matsumoto, T., Yamano, A., Niwa, K., Watanabe, T., Ohyama, T.
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Journal Title
Acta Crystallographica Section F
Volume: 68
Issue: 2
Pages: 227-230
DOI
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Peer Reviewed
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[Journal Article] Toxic and nontoxic components of botulinum neurotoxin complex are evolved from a common ancestral zinc protein2012
Author(s)
Inui, K., Sagane, Y., Miyata, K., Miyashita, S., Suzuki, T., Sikamori, Y., Ohyama, T., Niwa, K., Watanabe, T.
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 419
Issue: 3
Pages: 500-504
DOI
Related Report
Peer Reviewed
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[Journal Article] Characterization of sugar recognition by the toxin complex produced by Clostridium botulinum serotype C variant strain Yoichi2011
Author(s)
Matsuo, T., Miyata, K., Inui, K., Ito, H., Horiuchi, R., Suzuki, T., Yoneyama, T., Oguma, K., Niwa, K., Watanabe, T., Ohyama, T.
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Journal Title
FEMS Immunology Medicul Microbiology
Volume: 63
Issue: 1
Pages: 35-43
DOI
Related Report
Peer Reviewed
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[Journal Article] HA-33 facilitates transport of the serotype D botulinum toxin across a rat intestinal epithelial cell monolayer2011
Author(s)
Ito, H., Sagane, Y., Miyata, K., Inui, K., Matsuo, T., Horiuchi, R., Ikeda, T., Suzuki, T., Hasegawa, K., Kouguchi, K., Oguma, K., Niwa, K., Ohyama, T., Watanabe T.
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Journal Title
FEMS Immunology Medicul Microbiology
Volume: 61
Issue: 3
Pages: 323-331
DOI
Related Report
Peer Reviewed
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[Presentation] Transport of the botulinum neurotoxin-associating protein, nontoxic nonhemagglutinin, across the intestinal epithelial cell layer2012
Author(s)
Miyashita, S. -I., Sagane, Y., Miyata, K., Inui, K., Kurihara, S., Kano, S., Hayashi, S., Kawane, T., Suzuki, T., Niwa, K., and Watanabe, T
Organizer
22nd IUBMB and 37th FEBS
Place of Presentation
Seville, Spain
Related Report
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[Presentation] ボツリヌス神経毒素結合タンパク質非毒非血球凝集素の小腸上皮細胞への結合および透過2012
Author(s)
宮下慎一郎,相根義昌,宮田恵多,犬井健,栗原小百合,狩野聡史,林慎太郎,川根武紘,鈴木智典,丹羽光一,渡部俊弘
Organizer
第79回日本細菌学会北海道支部会学術総会
Place of Presentation
帯広
Related Report
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[Presentation] C 型ボツリヌス毒素の小腸上皮細胞からの侵入機構の解析2010
Author(s)
犬井 健,宮田恵多,伊藤博章,松尾 5,堀内亮太,吉田悠真,宮下慎一郎,村松将司,渡部俊弘,大山 徹,丹羽光一
Organizer
第33回日本分子生物学会年会代 83回日本生化学会大会合同大会
Place of Presentation
神戸
Related Report
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[Presentation] C 型およびD 型ボツリヌス毒素の小腸上皮細胞の透過におけるシアル酸の役割2010
Author(s)
丹羽光一,伊藤博章,犬井 健,宮田恵多,松尾智仁,堀内亮太,宮下慎一郎,吉田悠真,村松将司,渡部俊弘,大山 徹
Organizer
第150回日本獣医学会学術集会
Place of Presentation
帯広
Related Report
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[Book] Structure and function of food engineering. Ayman Amer Eissa2012
Author(s)
Sagane, Y., Inui, K., Miyashita, S. -I., Miyata, K., Suzuki, T., Niwa, K., and Watanabe, T
Publisher
Botulinum toxin complex: a delivery vehicle of botulinum neurotoxin traveling digestive tract
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