| Project/Area Number |
22590406
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
OHNO Naohito 東京薬科大学, 薬学部, 教授 (80152213)
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| Co-Investigator(Renkei-kenkyūsha) |
ADACHI Yoshiyuki (ISHIBASHI Ken-ichi / MIURA Noriko) 東京薬科大学, 薬学部, 准教授 (60222634)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 感染免疫 / PAMPs / 感染症 / 自然免疫 / βグルカン / αマンナン / 免疫毒性 / マンナン / dectin-1 / dectin-2 / 肝臓 / 遺伝子発現 / apoptosis / 真菌 / マイクロアレイ / 血管炎 / 抗炎症作用 / DBA/2マウス |
|---|
| Research Abstract |
DBA/2 is a strain, specifically higher response to fungal PAMPs, beta-glucan. In the present study, immunotoxicological and immunopathological rolesof fungal PAMPs were examined. From the results of this study, we have demonstratedthat DBA/2 mice is a highly sensitive strain to produce high level of inflammatory cytokinein vitro and in vito. In vitro study, DBA/2 mice produced significantly high level of inflammatory cytokines, such as GM-CSF, IFN-gamma, and TNF-alpha. It is highly sensitive tothe cross-linking of cytoskeleton and modulated by disrupting actin polymerization. In vivo study, producing high concentration of anti-beta-glucan antibody and induce lethal toxicity. From these findings, it is a good tool to analyze beta-glucan mediated immunotoxicological response.
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