Pathophysiologial role of Helicobacter pylori asparaginase

• Project/Area Number: 22590410
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: SHIBAYAMA Keigo 国立感染症研究所, 細菌第二部, 部長 (50283437)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: ヘリコバクターピロリ / アスパラギナーゼ / 代謝酵素 / 病原性 / 感染成立 / アスパラギン取り込み / 免疫回避 / 胃がん / ピロリ菌 / 胃癌 / 胃炎 / 胃潰瘍 / アスパラギン代謝

Research Abstract

Helicobacter pylori sparaginase exhibited striking cytotoxic activity against U937 cells via asparagine deprivation. The cytotoxic activity of live H. pylori cells against U937 cells was significantly diminished by deletion of the asparaginase gene. An asparaginase-deficient mutant strain was significantly less capable of colonizing Mongolian gerbils. H. pylori cells incorporated extracellular asparagine by transporting aspartate produced by the action of the asparaginase. H. pylori asparaginase may be involved in inhibition of normal lymphocyte function at the gastric niche, allowing H. pylori to evade the host immune system.

Research Products

• [Journal Article] Helicobacter cinaedi and H. fennelliae transmission in a hospital from 2008 to 2012. (2013)
  • Author(s): Rimbara E, Mori S, Kim H, Matsui M, Suzuki S, Takahashi S, Yamamoto S, Mukai M, Shibayama K.
  • Journal Title: J Clin Microbiol Volume: in press Issue: 7 Pages: 2439-42
  • DOI: 10.1128/jcm.01035-13
  • Peer Reviewed
• [Journal Article] Biochemical and pathophysiological characterization of Helicobacter pylori asparaginase. (2012)
  • Author(s): Shibayama K
  • Journal Title: Microbiology and Immunology Volume: 56(6) Issue: 6 Pages: 422-422
  • DOI: 10.1111/j.1348-0421.2012.00459.x
  • NAID: 10031121485
• [Journal Article] Molecular epidemiologic analysis and antimicrobial resistance of Helicobacter cinaedi isolated from seven hospitals in Japan. (2012)
  • Author(s): Rimbara E, Mori S, Matsui M, Suzuki S, Wachino J, Kawamura Y, Shen Z, Fox JG, Shibayama K
  • Journal Title: J Clin Microbiol Volume: 50(8) Issue: 8 Pages: 2553-60
  • DOI: 10.1128/jcm.06810-11
  • Peer Reviewed
• [Journal Article] Biochemical and pathophysiological characterization of Helicobacter pylori asparginase (2011)
  • Author(s): Shibayama K., Takeuchi H, Wachino J, Mori S, Arakawa Y
  • Journal Title: Microbiol.Immunol. Volume: 56 Issue: 6 Pages: 408-417
  • DOI: 10.1111/j.1348-0421.2011.00333.x
  • Peer Reviewed
• [Presentation] Role of gamma-glutamyl transpeptidase and asparaginase in the pathogenesis of Helicobacter pylori (2013)
  • Author(s): 林原 絵美子,森 茂太郎,金 玄,柴山 恵吾
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張
• [Presentation] Role of gamma-glutamyl transpeptidase and asparaginase in the pathogenesis of Helicobacter pylori (2013)
  • Author(s): 林原 絵美子
  • Organizer: 第８６回日本細菌学会総会
  • Place of Presentation: 千葉
• [Presentation] Molecular epidemiologic analysis and antimicrobial resistance of Helicobacter cinaedi isolated from 7 hospitals in Japan. (2012)
  • Author(s): E. Rimbara, S. Mori, M. Matsui, S. Suzuki, J. Wachino, Y. Kawamura, Z. Shen, J. G. Fox, K. Shibayama
  • Organizer: XXVth International Workshop on Helicobacter and related bacteria in chronic digestive inflammation and gastric cancer
  • Place of Presentation: Ljubljana, Slovenia
• [Presentation] 同一の病院で分離されたH. cinaediとH. fennelliaeの分子疫学的解析と薬剤感受性 (2012)
  • Author(s): 林原 絵美子,森 茂太郎,金 玄,松井 真理,鈴木 里和,高橋 俊司, 山本 聡,向井 正也,柴山 恵吾
  • Organizer: 第18回日本ヘリコバクター学会学術集会
  • Place of Presentation: 岡山
• [Presentation] Molecular epidemiologic analysis and antimicrobial resistance of Helicobacter cinaedi isolated from 7 hospitals in Japan (2012)
  • Author(s): E. Rimbara
  • Organizer: XXVth International Workshop on Helicobacter and related bacteria in chronic digestive inflammation and gastric cancer
  • Place of Presentation: スロベニア
• [Presentation] 同一の病院で分離されたH. cinaediとH. fennelliaeの分子疫学的解析と薬剤感受性 (2012)
  • Author(s): 林原 絵美子
  • Organizer: 第１８回日本ヘリコバクター学会学術集会
  • Place of Presentation: 岡山
• [Presentation] PATHOPHYSIOLOGICAL ROLE OF HELICOBACTEE PYLOEI γ-GLUT AMYLTRANSPEPTIDASE AND ASPARAGINASE (2011)
  • Author(s): Shibayama K
  • Organizer: International Union of Microbiological Societies 2011 Congress(ワークショップ)
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-08
• [Presentation] Molecular Epidemiology of Helicobacter cinaedi isolates in Japan. (2011)
  • Author(s): Rimbara E, Shibayama K
  • Organizer: XIII International Union of Microbiological Societies 2011 Congress (IUMS)
  • Place of Presentation: Sapporo, JAPAN
• [Presentation] Pathophysiological Role of Helicobacter pylori Asparaginase. (2011)
  • Author(s): Shibayama K, Takeuchi H, Wachino J, Mori S, Arakawa Y
  • Organizer: XIII International Union of Microbiological Societies 2011 Congress (IUMS)
  • Place of Presentation: Sapporo, Japan