Novel internal initiation mechanism of translation of h e p a t i t i s C virus RNA

• Project/Area Number: 22590413
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Jichi Medical University
• Principal Investigator: KAMOSHITA Nobuhiko 自治医科大学, 医学部, 講師 (90302603)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: C型肝炎ウイルス / RNA / 翻訳(translation) / 内部認識翻訳開始部位(internal ribosomal entry site) / 転写後遺伝子発現 / 選択的スプライシング / termination readthrough / 翻訳 / 内部認識翻訳開始配列(IRES) / 発現制御 / 選択的ポリA付加 / 翻訳終止 / 内部認識翻訳開始機構(IRES)

Research Abstract

Activityof putative novel internal ribosomal entry mechanism of translation initiation within hepatitis C virus (HCV) RNA was quantified in cultured cells in which mutant internal ribosomal entry site (IRES) is expressed. Recombinant virus vector, which h

Research Products

• [Presentation] ST2 and its related mRNA 図2.1 PSIV IGR IRESのA. 塩基配列、B.構造と、C. 構造の変化. A 中の矢印は表2.1で使用した欠失変異体の塩基残存部を示す.表2.1 PSIV IGR欠失変異体のreadthrough効率IGR 長 第2シストロン相対活性141 nt 1.0066 nt 5.8345 nt 10.11expression in human cell lines (2012)
  • Author(s): Kamoshita N, Tamemoto H, Hayakawa M, Tominaga S
  • Organizer: International Symposium in honor of Professor Uttam RajBhandary: From Innovations in Nu-cleic acids research to Regulation of Biological Processes
  • Place of Presentation: インド, バンガロール
  • Year and Date: 2012-12-16
• [Presentation] Effects of ASF/SF2 and HuR/ELAV1 on alternative splicing in ST2 gene. (2012)
  • Author(s): Kamoshita N, Tamemoto H, Hayakawa M, Tominaga S
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-15
• [Presentation] IL-33受容体ST2L/IL1RL1遺伝子座における選択的スプライシング. (2012)
  • Author(s): 鴨下信彦, 為本浩至, 早川盛禎, 富永眞一
  • Organizer: 第14回日本RNA学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2012-07-19
• [Presentation] IL-33受容体ST2L/IL1RL1遺伝子座における選択的スプライシング (2012)
  • Author(s): 鴨下信彦, 為本浩至, 早川盛禎, 富永眞一
  • Organizer: 第14回日本RNA学会年会
  • Place of Presentation: 東北大学百周年記念会館川内萩ホール (宮城県)
• [Presentation] ST2遺伝子の選択的スプライシングにおけるトランス因子の解析 (2012)
  • Author(s): Kamoshita, Tamamoto, Hayakawa, Tominaga
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: マリンメッセ福岡 (福岡県)
• [Presentation] ST2 and its related mRNA expression in human cell lines (2011)
  • Author(s): Kamoshita, Tamamoto, 他
  • Organizer: International Symposium in honor of Professor Uttam RajBhandary : From Innovations in Nucleic acids research to Regulation of Biological Processes
  • Place of Presentation: インド理科大学院(インド、バンガロール)
  • Year and Date: 2011-12-16
• [Presentation] Alternative splicing in ST2 gene (2011)
  • Author(s): Kamoshita N, Tamemoto H, Hayakawa M, Tominaga S.
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] Alternative splicing in ST2 gene (2011)
  • Author(s): Kamoshita, Tamamoto, 他
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-14
• [Presentation] Ectodomain shedding of ST2L, a member of IL1 receptor family (2010)
  • Author(s): Tamemoto, Ozaki, Kamoshita, 他
  • Organizer: BMB 2010
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-08
• [Book] 生化学事典IRESの項 (2013)
  • Author(s): 田中啓二, 他編
  • Total Pages: 1
  • Publisher: 朝倉書店
• [Book] 生化学事典 IRESの項 (2011)
  • Author(s): 田中啓二, 他編
  • Total Pages: 1
  • Publisher: 朝倉書店