How does cystatin-C enhance CD4-independent HIV infection?
| Project/Area Number |
22590416
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Nagasaki University |
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Principal Investigator |
KUBO Yoshinao 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (30273527)
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| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | HIV / cystatin-C / カテプシンB / 自然免疫 / ヒト免疫不全症ウイルス / エンドソーム / インターフェロン / エンドサイトーシス |
|---|
| Research Abstract |
HeLa cells were resistant to CD4-independent HIV infection. We identified cystatin-C (cathepsin protease inhibitor) that conferred HeLa cells susceptible to CD4-independent HIV infection. When target cells were treated with a low molecular weight cathepsin inhibitor, CA-074Me, CD4-independent HIV infection was enhanced. Because CD4-independent HIV infection was attenuated by an endocytosis inhibitor, the infection occurs through endosomes. When cathepsin activity is relatively higher, CD4-independent HIV infectivity may be reduced due to the degradation of HIV particlesincorporated into endosomes by cathepsin. Although murine leukemia virus (MLV) infection occurs via endosomes, MLV infection was rather suppressed by CA-074Me. This result indicates that cathepsin is required for MLV infection unlike CD4-independent HIV infection. Because MLV infection in XC cells is not suppressed by endosome acidificationinhibitors, it is widely accepted that MLV infection does not occur via endosomes specifically in XC cells. We found that MLV infection in XC cells occurs through endosomes, and cathepsin activated without endosome acidification in XC cells confers MLV infection resistant to endosome acidification inhibitors.
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] CXCR4-tropic, but not CCR5-tropic, human immunodeficiency virus infection is inhibited by the lipid raft-associated factors, acyclic retinoid analogs, and cholera toxin B subunit2013
Author(s)
H. Kamiyama, K. Kakoki, S. Shigematsu, M. Izumida, Y. Yashima, Y. Tanaka, H. Hayashi, T. Matsuyama, H. Sato, N. Yamamoto, T. Sano, Y. Shidoji, Y. Kubo
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Journal Title
AIDS Res.Hum.Retrovir.
Volume: 29
Issue: 2
Pages: 279-88
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] CD4-independent human immunodeficiency virus infection involves participation of endocytosis and cathepsin B2011
Author(s)
H. Yoshii, H. Kamiyama, K. Goto, K. Oishi, N. Katsunuma, Y. Tanaka, H. Hayashi, T. Matsuyama, H. Sato, N. Yamamoto, Y. Kubo
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Journal Title
PLoS ONE
Volume: 6
Issue: 4
Pages: e19352-e19352
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Surfactant protein C G100S mutation causes familial pulmonary fibrosis in Japanese kindred2011
Author(s)
Ono S, Tanaka T, Ishida M, Kinoshita A, Fukuoka J, Takaki M, Sakamoto N,Ishimatsu Y, Kohno S, Hayashi T, Senba M, Yasunami M, Kubo Y, Yoshida LM, Kubo H, Ariyoshi K, Yoshiura K, Morimoto K.
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Journal Title
Eur Respir J
Volume: 38
Issue: 4
Pages: 861-869
DOI
Related Report
Peer Reviewed
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[Journal Article] Interferon regulatory factor-4 activates IL-2 and IL-4 promoters in cooperation with c-Rel2011
Author(s)
H. Shindo, K. Yasui, K. Yamamoto, K. Honma, K. Yui, T. Kohno, Y. Ma, K.J. Chua, Y. Kubo, H. Aihara, T. Ito, T.Nagayasu, T. Matsuyama, H. Hayashi.
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Journal Title
Cytokine
Volume: 56
Issue: 3
Pages: 564-72
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Surfactant protein C G100S mutation causes familial pulmonary fibrosis in Japanese kindred2011
Author(s)
S.Ono, T.Tanaka, M.Ishida, A.Kinoshita, J.Fukuoka, M.Takaki, N.Sakamoto, Y.Ishimatsu, S.Kohno, T.Hayashi, M.Senba, M.Yasunami, Y.Kubo, L.M.Yoshida, H.Kubo, K.Ariyoshi, K.Yoshiura, K.Morimoto
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Journal Title
European Respiratory Journal
Volume: (In press 掲載確定)
Related Report
Peer Reviewed
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