RBM15-mediated mRNA export

• Project/Area Number: 22590418
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Nagoya City University
• Principal Investigator: URANISHI Hiroaki 名古屋市立大学, 大学院・医学研究科, 研究員 (40363923)
• Co-Investigator(Kenkyū-buntansha): OKAMOTO Takashi 名古屋市立大学, 大学院・医学研究科, 教授 (40146600)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: RBM15 / mRNA 輸送 / NXF1 / mRNA輸送 / 転写後調節 / ウイルス

Research Abstract

We report that RBM15 binds to ORF57 and participates in ORF57-enhanced expression of KSHV ORF59. RBM15 binds to NXF1 via its SPOC domain and promotes mRNA association to the NXF1 export pathway. Thus RBM15 is considered to be a crucial factor for mRNA export as a component of the NXF1 pathway.

Research Products

• [Journal Article] The Kaposi's Sarcoma-associated Herpesvirus ORF57 Is Not a bona fide Export Factor (2012)
  • Author(s): Pilkington GR, Majerciak V, Bear J, Uranishi H, Zheng ZM, Felber BK.
  • Journal Title: J Virol. Volume: Vol.86 Issue: 23 Pages: 13089-94
  • DOI: 10.1128/jvi.00606-12
  • Peer Reviewed
• [Journal Article] Kaposi's sarcoma-associated herpesvirus ORF57 interacts with cellular RNA export cofactors RBM15 and OTT3 to promote expression of viral ORF59. (2011)
  • Author(s): Majerciak V, Uranishi H, Kruhlak M, Pilkington GR, Massimelli MJ, Bear J, Pavlakis GN, Felber BK, Zheng ZM.
  • Journal Title: J Virol. Volume: Vol.85 Issue: 4 Pages: 1528-40
  • DOI: 10.1128/jvi.01709-10
  • Peer Reviewed
• [Journal Article] Kaposi's Sarcoma-Associated Herpesvirus ORF57 Interacts with Cellular RNA Export Cofactors RBM15 and OTT3 To Promote Expression of Viral ORF59 (2011)
  • Author(s): Vladimir Majerciak, Hiroaki Uranishi, Michael Kruhlak, Guy R.Pilkington, Maria Julia Massimelli, Jenifer Bear, George N.Pavlakis, Barbara K.Felber, Zhi-Ming Zheng
  • Journal Title: Journal of Virology Volume: 85 Pages: 1528-1540
  • Peer Reviewed
• [Journal Article] The RNA transport element of the murine musD retrotransposon requires long-range intramolecular interactions for function. (2010)
  • Author(s): Legiewicz M, Zolotukhin AS, Pilkington GR, Purzycka KJ, Mitchell M, Uranishi H, Bear J, Pavlakis GN, Le Grice SF, Felber BK.
  • Journal Title: J Biol Chem Volume: Vol.285 Issue: 53 Pages: 42097-104
  • DOI: 10.1074/jbc.m110.182840
  • Peer Reviewed
• [Journal Article] The RNA Transport Element of the Murine musD Retrotransposon Requires Long-range Intramolecular Interactions for Function (2010)
  • Author(s): Michal Legiewicz, Andrei S.Zolotukhin, Guy R.Pilkington, Katarzyna J.Purzycka, Michelle Mitchell, Hiroaki Uranishi, Jenifer Bear, George N.Pavlakis, Stuart F.J.Le Grice, Barbara K.Felber
  • Journal Title: Journal of Biological Chemistry Volume: 285 Pages: 42097-42104
  • Peer Reviewed
• [Presentation] The RNA binding motif protein 15 (RBM15) binds to NXF1 and promotes posttranscriptional regulation of gene expression. (2012)
  • Author(s): 浦西宏明, George N. Pavlakis, Barbara K. Felber, 岡本 尚
  • Organizer: 第76回 日本生化学会中部支部例会
  • Place of Presentation: 自然科学研究機構 岡崎コンファレンスセンター(愛知県)
  • Year and Date: 2012-05-26
• [Presentation] The RNA binding motif protein 15 (RBM15) binds to NXF1 and promotes posttranscriptional regulation of gene expression (2012)
  • Author(s): 浦西宏明
  • Organizer: 第76回 日本生化学会 中部支部例会
  • Place of Presentation: 自然科学研究機構 岡崎コンファレンスセンター（ 愛知県）