Characterization of cell culture-generated hepatitis E virus particles:evaluation of neutralizing antibodies-inducing ability as the material of inactivated vaccineproduction

• Project/Area Number: 22590419
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Jichi Medical University
• Principal Investigator: TAKAHASHI Masaharu 自治医科大学, 医学部, 講師 (70326841)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: E型肝炎ウイルス / ワクチン / 中和抗体 / 培養細胞

Research Abstract

HEV particles generated in culture supernatant of cell-culturesystems of PLC/PRF/5, A549, HepG2/C3A, Li-7, Huh7 and Caco-2 cells were found to beassociated with envelope-like membrane, and HEV capsid antigen which carryneutralization epitope was buried by

Research Products

• [Journal Article] Frequent detection and characterization of hepatitis E virus variants in wild rats (Rattus rattus) in Indonesia (2013)
  • Author(s): Mulyanto
  • Journal Title: Archives of Virology Volume: 158 Issue: 1 Pages: 87-96
  • DOI: 10.1007/s00705-012-1462-0
  • Peer Reviewed
• [Journal Article] A549 and PLC/PRF/5 cells can support the efficient propagation of swine and wild boar hepatitis E virus (HEV) strains : demonstration of HEV infectivity of porcine liver sold as food (2012)
  • Author(s): Takahashi H
  • Journal Title: Archives of Virology Volume: 157 Issue: 2 Pages: 235-246
  • DOI: 10.1007/s00705-011-1153-2
  • Peer Reviewed
• [Journal Article] Icteric acute hepatitis E with no response of immunoglobulin M class anti-hepatitis E virus antibody (2012)
  • Author(s): Takikawa Y
  • Journal Title: Hepatology Research Volume: 42 Issue: 11 Pages: 1146-1149
  • DOI: 10.1111/j.1872-034x.2012.01023.x
  • Peer Reviewed
• [Journal Article] Molecular epidemiology and genetic history of European-type genotype 3 hepatitis E virus indigenized in the central region of Japan (2012)
  • Author(s): Nakano T
  • Journal Title: Infection, Genetics and Evolution Volume: 12 Issue: 7 Pages: 1524-1534
  • DOI: 10.1016/j.meegid.2012.06.002
  • Peer Reviewed
• [Journal Article] Molecular analysis of hepatitis E virus from farm rabbits in Inner Mongolia, China and its successful propagation in A549 and PLC/PRF/5 cells (2012)
  • Author(s): Jirintai S
  • Journal Title: Virus Research Volume: 170 Issue: 1-2 Pages: 126-137
  • DOI: 10.1016/j.virusres.2012.09.015
  • Peer Reviewed
• [Journal Article] A PSAPmotif in the ORF3 protein of hepatitis E virusis necessary for virion release from infectedcells (2011)
  • Author(s): Nagashima S, Takahashi M, Jirintai, Tanaka T,Yamada K, Nishizawa T, Okamoto H
  • Journal Title: J Gen Virol Volume: 92 Issue: 2 Pages: 269-278
  • DOI: 10.1099/vir.0.025791-0
  • Peer Reviewed
• [Journal Article] Analysis of the full-length genome of a hepatitis E virus isolate obtained from a wild boar in Japan that is classifiable into a novel genotype (2011)
  • Author(s): Takahashi M
  • Journal Title: Journal of General Virology Volume: 92 Issue: 4 Pages: 902-908
  • DOI: 10.1099/vir.0.029470-0
  • Peer Reviewed
• [Journal Article] A nationwide survey of hepatitis E virus (HEV) infection in wild boars in Japan : identification of boar HEV strains of genotypes 3 and 4 and unrecognized genotypes (2011)
  • Author(s): Sato Y
  • Journal Title: Archives of Virology Volume: 156 Issue: 8 Pages: 1345-1358
  • DOI: 10.1007/s00705-011-0988-x
  • Peer Reviewed
• [Journal Article] Tumour susceptibility gene 101 and the vacuolar protein sorting pathway are required for the release of hepatitis E virions (2011)
  • Author(s): Nagashima S
  • Journal Title: Journal of General Virology Volume: 92 Issue: 12 Pages: 2838-2848
  • DOI: 10.1099/vir.0.035378-0
  • Peer Reviewed
• [Journal Article] 北海道東部の釧路市および根室市におけるE型肝炎ウイルス感染の疫学調査:感染の地域差と食文化の相違について (2011)
  • Author(s): 田辺利男, 水尾仁志, 矢崎康幸, 高橋雅春, 岡本宏明
  • Journal Title: 肝臓 Volume: 52 Pages: 567-574
  • NAID: 10029688070
  • Peer Reviewed
• [Journal Article] A PSAP motif in the ORF3 protein of hepatitis E virus is necessary for virion Release from infected cells. (2011)
  • Author(s): Nagashima S
  • Journal Title: Journal of General Virology Volume: 92 Pages: 269-278
  • Peer Reviewed
• [Journal Article] Analysis of the full-length genome of a hepatitis E virus isolate obtained from a wild boar in Japan that is classifiable into a novel genotype. (2011)
  • Author(s): Takahashi M
  • Journal Title: Journal of General Virology Volume: 92 Pages: 902-908
  • Peer Reviewed
• [Journal Article] Hepatitis Evirus (HEV) strains in serum samples canreplicate efficiently in cultured cells despitethe coexistence of HEV antibodies:characterization of HEV virions in bloodcirculation. (2010)
  • Author(s): Takahashi M, Tanaka T, Takahashi H,Hoshino Y, Nagashima S, Jirintai, Mizuo H,Yazaki Y, Takagi T, Azuma M, Kusano E,Isoda N, Sugano K, Okamoto H
  • Journal Title: J Clin Microbiol Volume: 48 Issue: 4 Pages: 1112-1125
  • DOI: 10.1128/jcm.02002-09
  • Peer Reviewed
• [Journal Article] Molecular andserological survey of hepatitis E virusinfection among domestic pigs in InnerMongolia (2010)
  • Author(s): Jinshan, Jirintai, Manglai D, Takahashi M,Nagashima S, Okamoto H
  • Journal Title: China. Arch Virol Volume: 155 Issue: 8 Pages: 1217-1226
  • DOI: 10.1007/s00705-010-0706-0
  • Peer Reviewed
• [Journal Article] 札幌圏内小流行4 型HEV株が検出された茨城県内E型肝炎の1例 (2010)
  • Author(s): 相川達也, 池澤和人, 間宮孝, 上野ちさと, 和田由美子, 島田沙香, 津田文男, 高橋雅春, 岡本宏明
  • Journal Title: 肝臓 Volume: 51 Pages: 579-581
  • NAID: 10027469901
  • Peer Reviewed
• [Journal Article] Hepatitis E virus (HEV) strains in serum samples can replicate efficiently in cultured cell despite the coexistence of HEV antibodies : characterization of HEV virions in blood circulation. (2010)
  • Author(s): Takahashi M
  • Journal Title: Journal of Clinical Microbiology Volume: 48 Pages: 1112-1125
  • Peer Reviewed
• [Journal Article] Molecular and serological survey of hepatitis E virus infection among domestic pigs in Inner Mongolia, China. (2010)
  • Author(s): Jinshan
  • Journal Title: Archives of Virology Volume: 155 Pages: 1217-1226
  • Peer Reviewed
• [Journal Article] 札幌圏内小流行4型HEV株が検出された茨城県内E型肝炎の1例 (2010)
  • Author(s): 相川達也
  • Journal Title: 肝臓 Volume: 51 Pages: 579-581
  • Peer Reviewed
• [Book] 肝・胆道系症候群(第2版)I 肝臓編(上) E型肝炎 (2010)
  • Author(s): 高橋雅春, 岡本宏明
  • Total Pages: 600
  • Publisher: 日本臨牀社
• [Book] 肝・胆道系症候群(第2版)その他の肝・胆道系疾患を含めてI肝臓編(上) (2010)
  • Author(s): 高橋雅春(共著)
  • Publisher: 日本臨牀社
• [Remarks] 自治医科大学医学部 感染・免疫学講座 ウイルス学部門ホームページ
  • URL: http://www.jichi.ac.jp/virology/
• [Remarks] 自治医科大学医学部 感染・免疫学講座 ウイルス学部門
  • URL: http://www.jichi.ac.jp/virology/