| Project/Area Number |
22590420
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHIBATA Toshikatu 日本大学, 医学部, 研究員 (30398854)
|
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| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 細胞 / 出芽 / インフルエンザ / ライブセルイメージング / インフルエンザウイルス / 出芽過程 / 蛍光タンパク質 / テトラシステインタグ |
|---|
| Research Abstract |
By using fluorescence-protein-tagged M1 protein (GFP-M1) and total internal reflection microscope, we tried to observe the process of influenza viral particle formation in live cells. When GFP-M1 expressing cells were infected with the virus, punctate structure of GFP-M1 was observed after 8 hours infection, and then the structures tended to aggregate. Another viral protein, HA showd the same tendency. The results indicated the punctate sturucture of GFP-M1 corresponded to theformation of viral particle.
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