| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
In this study we showed that 1) Japanese encephalitis virus (JEV)with a 3-Ile residue in NS4A protein exhibits higher neuroinvasiveness than JEV with 3-Val in the protein, 2) approximately 20% of the JEV isolates in Japan have 3-Ile residue in NS4A, and in the 3-Ile group we found a strain with significantly weaker virulence as compared with the other 3-Ile strains, 3) a Ser-to-Asn substitution at position 123 in the JEV E protein has weak impact on viral growth properties in vitro or on pathogenicity in vivo, 4) the short VR in JEV 3’ non-translated region do not affect its growth in vitro or its pathogenicity in mice.
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