Analysis of the role of Japanese encephalitis virus NS4A protein in its pathogenicity
Project/Area Number |
22590423
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Virology
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Research Institution | National Institute of Infectious Diseases |
Principal Investigator |
TAJIMA Shigeru 国立感染症研究所, ウイルス第一部, 主任研究官 (60311346)
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Project Period (FY) |
2010 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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Keywords | 日本脳炎ウイルス / NS4A / 病原性 |
Research Abstract |
In this study we showed that 1) Japanese encephalitis virus (JEV)with a 3-Ile residue in NS4A protein exhibits higher neuroinvasiveness than JEV with 3-Val in the protein, 2) approximately 20% of the JEV isolates in Japan have 3-Ile residue in NS4A, and in the 3-Ile group we found a strain with significantly weaker virulence as compared with the other 3-Ile strains, 3) a Ser-to-Asn substitution at position 123 in the JEV E protein has weak impact on viral growth properties in vitro or on pathogenicity in vivo, 4) the short VR in JEV 3’ non-translated region do not affect its growth in vitro or its pathogenicity in mice.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] serine-to-asparagine substitution at position 123 in the Japanese encephalitis virus E protein.2013
Author(s)
Yamaguchi, Y., Nukui, Y., Kotaki, A., Sawabe, K., Saijyo, M., Watanabe, H., Kurane, I., Takasaki, T., and Tajima, S
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Journal Title
Journal of General Virology
Volume: 94
Pages: 90-96
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[Journal Article] Identification and characterization of the short variable region of the Japanese encephalitis virus 3' NTR.2012
Author(s)
2)Kato, F., Kotaki, A., Yamaguchi, Y., Shiba, H., Hosono, K., Harada, S., Saijo, M, Kurane, I., Takasaki, T., and Tajima, S
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Journal Title
Virus Genes
Volume: 44
Pages: 191-197
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[Journal Article] Identification and characterization of the short variable region of the Japanese encephalitis virus 3' NTR2012
Author(s)
Kato, F., Kotaki, A., Yamaguchi, Y., Shiba, H., Hosono, K., Harada, S., Saijo, M Kurane, I., Takasaki, T., Tajima, S.
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Journal Title
Virus Genes
Volume: 44
Pages: 191-197
Related Report
Peer Reviewed
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[Journal Article] An amino acid substitution (V3I) in the Japanese encephalitis virus NS4A protein increases its virulence in mice, but not its growth rate in vitro.2011
Author(s)
Yamaguchi, Y., Nukui, Y., Tajima, S., Nerome, R., Kato, F., Watanabe, H., Takasaki, T., and Kurane, I.
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Journal Title
Journal of General Virology
Volume: 92
Pages: 1601-1606
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[Journal Article] An amino acid substitution (V3I) in the Japanese encephalitis virus NS4A protein increases its virulence in mice, but not its growth rate in vitro2011
Author(s)
Yamaguchi, Y., Nukui, Y, Tajima, S., Nerome, R., Kato, F., et al.
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Journal Title
Journal of General Virology
Volume: 92
Pages: 1601-1606
Related Report
Peer Reviewed
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[Journal Article] Common marmoset (Callithrix jacchus) as a primate model of dengue virus infection : development of high levels of viremia and demonstration of protective immunity2011
Author(s)
Omatsu, T., Moi, M.L., Hirayama, T., Takasaki, T., Nakamura, S., Tajima, S., et al.
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Journal Title
Journal of General Virology
Volume: 92
Pages: 2272-2280
Related Report
Peer Reviewed
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[Journal Article] Dengue virus isolation relying on antibody-dependent enhancement mechanism using FcgR-expressing BHK cells and a monoclonal antibody with infection-enhancing capacity2011
Author(s)
Moi, M.L., Lim, C.K., Tajima, S., Kotaki, A., Saijo, M., Taaksaki, T., Kurane, I.
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Journal Title
Journal of Clinical Virology
Volume: 52
Pages: 225-230
Related Report
Peer Reviewed
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[Presentation] Effect of a single amino acd substitution (S123N) of the Japanese encephalitis virus E protein on its growth in vitro2011
Author(s)
Yamaguchi, Y., Kotaki, A., Sawabe, K Watanabe, H., Kurane, I., Takasaki, T., Tajima, S.
Organizer
International Union of Microbiological Societies 2011 Congress
Place of Presentation
札幌コンベンションセンター(札幌市)
Year and Date
2011-09-15
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[Presentation] Effects of single amino acid substitution at position 123 in the Japanese encephalitis virus E protein on its growth rate in vitro and pathogenicity in mice.2010
Author(s)
Tajima, S., Yamaguchi, Y., Kato, F., Takasaki, T., Kurane, I.
Organizer
1^<st> Asia-Pacific Workshop on Neurovirology
Place of Presentation
Seoul National University, (Seoul, South Korea)
Year and Date
2010-07-16
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