| Project/Area Number |
22590426
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
INOUE Naoki 国立感染症研究所, ウイルス第一部, 室長 (90183186)
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| Research Collaborator |
HASHIMOTO Kaede 国立感染症研究所, ウイルス第一部, 研究生
FUKUCHI Saki 国立感染症研究所, ウイルス第一部, 研究生
FUKUI Yoshiko 国立感染症研究所, ウイルス第一部, 非常勤職員
TSUDA Mihoko 国立感染症研究所, ウイルス第一部, 非常勤職員
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | サイトメガロウイルス / モルモット / 感染防御 / 糖蛋白 / 細胞指向性 / アデノウイルス / 中和抗体 |
|---|
| Research Abstract |
In contrast to murine and rat CMVs, guinea pig CMV (GPCMV) crosses the placenta and causes infection in utero, which makes GPCMV animal models useful for studies on the mechanisms of transplacental transmission of CMV. Previously, we reported that the GPCMV stock purchased from the ATCC contained two types of strains, one containing and the other lacking a 1.6 kb locus that encodes human CMV UL128 and UL130 homologs, GP129 and GP131, and that the 1.6 kb locus was required for efficient viral growth in animals but not in cell culture. HCMV UL128/130/131A form a pentamer complex with gH/gL and play a role in the endothelial/epithelial-tropism. In this study, we constructed a BAC containing a GPCMV genome, the virus from which grew normally in guinea pig fibroblast cells GPL. Using the RedET recombination system for BAC, mutations were introduced into each of GP129, GP131, and GP133 ORFs. Although all mutants grew in GPL and epithelial cell line GPC-16 at the level similar to a virus derived from the BAC with the wild-type sequence, they could not infect monocytes/ macrophages. GPCMV was transmittable to fibroblast from infected macrophages by co-culturing. Since macrophages play an important role in dissemination of virus to the organs, the macrophage tropism may associate with efficient viral growth in vivo.
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