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Role of GANP-mediated hypermutation

Research Project

Project/Area Number 22590436
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Immunology
Research InstitutionKumamoto University

Principal Investigator

MAEDA Kazuhiko  熊本大学, 大学院・生命科学研究部, 助教 (20332869)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords免疫学 / ゲノム / 遺伝子
Research Abstract

Activation-induced cytidine deaminase (AID) is essential to thegeneration for somatic hypermutation at immunoglobulin gene locus. However, it is unclearhow cytoplasmic AID shepherds from cytoplasm to nucleus toward the IgV locus. We studiedthe role of GANP-mediated somatic hypermutation in B cells. We showed that GANP forms acomplex with AID in the cytoplasm and relocated into the nucleus. We found that GANPregulates the recruitment of AID to the immunoglobulin variable regions by modulatingtranscription and nucleosome occupancy.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (42 results)

All 2013 2012 2011 2010 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (24 results) Book (9 results) Remarks (3 results)

  • [Journal Article] GANP regulates recruitment ofAID to immunoglobulin variable regions bymodulating transcription and nucleosomeoccupancy2013

    • Author(s)
      Shailendra Kumar Singh, Kazuhiko Maeda(co-first author), Mohammed Mansour AbbasEid, Sarah Ameen Almofty, Masaya Ono,Phuong Pham, Myron F. Goodman, and NobuoSakaguchi
    • Journal Title

      Nat. Commun

      Volume: 4 Issue: 1 Pages: 1830-1830

    • DOI

      10.1038/ncomms2823

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic appearance of antigenic epitopes effective for viral neutralization during membrane fusion initiated by interactions between HIV-1 envelope proteins and CD4/CXCR42012

    • Author(s)
      Toda T, et al
    • Journal Title

      Immunobiology

      Volume: (印刷中) Issue: 9 Pages: 864-872

    • DOI

      10.1016/j.imbio.2011.12.007

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Mechanism of Immunoglobulin V-Region Diversification Regulated by Transcription and RNA Metabolism in Antigen-Driven B Cells2011

    • Author(s)
      Sakaguchi N, et al
    • Journal Title

      Scandinavian Journal of Immunology

      Volume: 73 Issue: 6 Pages: 520-526

    • DOI

      10.1111/j.1365-3083.2011.02557.x

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Mechanism of Immunoglobulin V-Region Diversification Regulated by Transcription and RNA Metabolism in Antigen-Driven B Cells2011

    • Author(s)
      Sakaguchi N, et al.
    • Journal Title

      Scandinavian Journal of Immunology

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GANP-mediated recruitment ofactivation-induced cytidine deaminase tocell nuclei and to immunoglobulin variableregion DNA2010

    • Author(s)
      Kazuhiko Maeda, Shailendra KumarSingh, Kazufumi Eda, Masahiro Kitabatake,Phuong Pham, Myron F. Goodman, and NobuoSakaguchi
    • Journal Title

      J. Biol. Chem

      Volume: 285 Issue: 31 Pages: 23945-23953

    • DOI

      10.1074/jbc.m110.131441

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] GANP-mediated recruitment of activation-induced cytidine deaminase to cell nuclei and to immunoglobulin variable region DNA2010

    • Author(s)
      Maeda K, et al.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 285 Pages: 23945-23953

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] GANP associated with HIV-1 RNA export is encapsidated with APOBEC3G into virion core2012

    • Author(s)
      Maeda K
    • Organizer
      Keystone Symposia Mutations, Malignancy and Memory-Antibodies and Immunity
    • Place of Presentation
      Boston Park Plaza & Towers
    • Year and Date
      2012-03-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] Role of GANP in transcriptional regulation of immunoglobulin gene locus2012

    • Author(s)
      Sakaguchi N
    • Organizer
      Keystone Symposia Mutations, Malignancy and Memory-Antibodies and Immunity
    • Place of Presentation
      Boston Park Plaza & Towers
    • Year and Date
      2012-03-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] Targeting the rearranged immunoglobulin V-region locus in B-cells by the histone acetyl transcriptase activity of GANP2012

    • Author(s)
      Kazuhiko Maeda
    • Organizer
      第6回日本エピジェネティクス研究会年会
    • Place of Presentation
      学術総合センター(東京一ツ橋)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Mammalian TREX-2 component GANP is involved in HIV-1 mRNA export2012

    • Author(s)
      Kazuhiko Maeda
    • Organizer
      The 13th Kumamoto AIDS Seminar GCOE Joint International Symposium
    • Place of Presentation
      Hotel Nikko Kumamoto, Aso Resort Grandvrio Hotel
    • Related Report
      2012 Annual Research Report
  • [Presentation] GANPを介したAPOBEC3Gの抗HIV感染阻止機構の解析2012

    • Author(s)
      前田和彦
    • Organizer
      第26回日本エイズ学会学術集会・総会
    • Place of Presentation
      慶応義塾大学 日吉キャンパス
    • Related Report
      2012 Annual Research Report
  • [Presentation] GANP regulates transcription and nucleosome occupancy at the immunoglobulin variable region for AID-accession2012

    • Author(s)
      Kazuhiko Maeda
    • Organizer
      The 41th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      Kobe International Conference Center Kobe International Exhibition Hall Kobe Portopia Hotel
    • Related Report
      2012 Annual Research Report
  • [Presentation] Regulation of immunoglobulin variableregion diversification through GANP-mediated regulation of DNA double-strand break repair machinery2012

    • Author(s)
      Mohammed Mansour Abbas Eid
    • Organizer
      The 41th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      Kobe International Conference Center Kobe International Exhibition Hall Kobe Portopia Hotel
    • Related Report
      2012 Annual Research Report
  • [Presentation] Escorting APOBEC3G toward the fulllength HIV-1 mRNA at the cytoplasmic P bodies2012

    • Author(s)
      Sarah Ameen Almofty
    • Organizer
      The 41th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      Kobe International Conference Center Kobe International Exhibition Hall Kobe Portopia Hotel
    • Related Report
      2012 Annual Research Report
  • [Presentation] GANP regulates APOBEC3G targeting for reduction of HIV-1 infectivity2012

    • Author(s)
      Shailendra Kumar Singh
    • Organizer
      The 41th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      Kobe International Conference Center Kobe International Exhibition Hall Kobe Portopia Hotel
    • Related Report
      2012 Annual Research Report
  • [Presentation] APOBEC3GのHIV-1粒子内への取り込みを制御する宿主因子GANP2011

    • Author(s)
      前田和彦
    • Organizer
      第25回日本エイズ学会学術集会
    • Place of Presentation
      ハイアットリージェンシー東京
    • Year and Date
      2011-12-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] Analysis of GANP-associated complex in B-cell nuclei2011

    • Author(s)
      Maeda K
    • Organizer
      The 40th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      幕張メッセ、千葉
    • Year and Date
      2011-11-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Involvement of GANP in transcriptional regulation of immunoglobulin gene locus2011

    • Author(s)
      Singh SK
    • Organizer
      The 40th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      幕張メッセ、千葉
    • Year and Date
      2011-11-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] GANP augments the immunoglobulin Vregion gene conversion via Ku70 dependent mechanism2011

    • Author(s)
      Eid MMA
    • Organizer
      The 40th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      幕張メッセ、千葉
    • Year and Date
      2011-11-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] GANP promotes APOBEC3G encapsidation in virions to reduce viral infectivity2011

    • Author(s)
      Maeda K
    • Organizer
      The 12th Kumamoto AIDS Seminar GCOE Joint International Symposium
    • Place of Presentation
      Hotel Nikko Kumamoto, Aso Resort, Grandvrio Hotel
    • Year and Date
      2011-10-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] GANP associated with HIV-1 RNA export is encapsidated with Apobec3G into virion cores2011

    • Author(s)
      Maeda K
    • Organizer
      The 2011 Meeting on Retroviruses at Cold Spring Harbor Laboratory
    • Place of Presentation
      Cold Spring Harbor, New York
    • Year and Date
      2011-05-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Transcriptional targeting at immunoglobulin V-region locus by an RNA-export molecule GANP2011

    • Author(s)
      前田和彦
    • Organizer
      第5回日本エピジェネティクス研究会年会
    • Place of Presentation
      KKRホテル熊本、熊本
    • Year and Date
      2011-05-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] Regulation of immune function by controlling RNA metabolism. The 2nd Workshop of Synthetic Immunology2010

    • Author(s)
      Sakaguchi N, et al.
    • Organizer
      The 2nd Workshop of Synthetic Immunology
    • Place of Presentation
      Kyoto University Shiran Kaikan, Inamori Hall
    • Year and Date
      2010-12-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] Apobec3のHIV-1粒子内への取り込みを制御する宿主因子の同定2010

    • Author(s)
      阪口薫雄, 他
    • Organizer
      第24回日本エイズ学会学術集会
    • Place of Presentation
      グランドプリンスホテル高輪/ザ・プリンスさくらタワー東京
    • Year and Date
      2010-11-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] GANP is a host factor that regulates Apobec3G encapsidation to HIV-1 viruses2010

    • Author(s)
      Sakaguchi N, et al.
    • Organizer
      Asian-African Research Forum 2010
    • Place of Presentation
      Melia Hotel, Hanoi
    • Year and Date
      2010-11-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] GANP-mediated recruitment of AID to the nuclei and to IgV-region DNA2010

    • Author(s)
      Maeda K, et al.
    • Organizer
      The 5th International Conference on Gene Regulation in Lymphocyte Development Aegean Conferences
    • Place of Presentation
      Minoa Palace Conference Center, Greece
    • Year and Date
      2010-10-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] GANP regulates the incorporation of Apobec3G into HIV-1 virions2010

    • Author(s)
      Maeda K, et al.
    • Organizer
      The 11^<th> Kumamoto AIDS Seminar GCOE Joint International Symposium
    • Place of Presentation
      Hotel Nikko Kumamoto, Aso Resort Grandvrio Hotel
    • Year and Date
      2010-10-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] GANP controls AID recruitment to immunoglobulin variable region DNA2010

    • Author(s)
      Maeda K, et al.
    • Organizer
      The 14th International Congress of Immunology
    • Place of Presentation
      The Kobe Convention Center Complex
    • Year and Date
      2010-08-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] A role of chromatin modification at immunoglobulin locus by GANP2010

    • Author(s)
      Singh SK, et al.
    • Organizer
      The 14th International Congress of Immunology
    • Place of Presentation
      The Kobe Convention Center Complex
    • Year and Date
      2010-08-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] Molecular mechanism of immunoglobulin V-region diversification and its application in medical sciences2010

    • Author(s)
      阪口薫雄, 他
    • Organizer
      日本ヒトプロテオーム機構第8回大会(日本プロテオーム学会2010年会)・第6回日本臨床プロテオーム研究会・連合大会
    • Place of Presentation
      東京ベイホテル東急
    • Year and Date
      2010-07-26
    • Related Report
      2010 Annual Research Report
  • [Book] 臨床免疫・アレルギー科2012

    • Author(s)
      前田和彦、阪口薫雄
    • Publisher
      科学評論社
    • Related Report
      2012 Final Research Report
  • [Book] B細胞の生存維持とmiRNA2012

    • Author(s)
      前田和彦
    • Publisher
      科学評論社
    • Related Report
      2012 Annual Research Report
  • [Book] プロテインホスファターゼ2Aの調節と自己免疫2012

    • Author(s)
      前田和彦, 他
    • Publisher
      科学評論社
    • Related Report
      2011 Annual Research Report
  • [Book] 実験医学、免疫記憶の制御と疾患/B 細胞免疫記憶 : 免疫記憶研究のフロンティア2011

    • Author(s)
      前田和彦、阪口薫雄
    • Publisher
      羊土社
    • Related Report
      2012 Final Research Report
  • [Book] 臨床免疫・アレルギー科2011

    • Author(s)
      前田和彦、阪口薫雄
    • Publisher
      科学評論社
    • Related Report
      2012 Final Research Report
  • [Book] 高親和性抗体産生のメカニズム2011

    • Author(s)
      前田和彦, 他
    • Publisher
      羊土社
    • Related Report
      2011 Annual Research Report
  • [Book] 胚中心B細胞の分化・増殖とGANP2011

    • Author(s)
      前田和彦, 他
    • Publisher
      科学評論社
    • Related Report
      2011 Annual Research Report
  • [Book] 臨床免疫・アレルギー科2010

    • Author(s)
      前田和彦、阪口薫雄
    • Publisher
      科学評論社
    • Related Report
      2012 Final Research Report
  • [Book] 抗体の抗原親和性成熟とGANPの役割2010

    • Author(s)
      前田和彦, 他
    • Publisher
      科学評論社
    • Related Report
      2010 Annual Research Report
  • [Remarks] 2013年5月15日、熊本日々新聞、「遺伝子変異させ強力な抗体」 ホームページ

    • URL

      http://www.k-immu.jp/ja/

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.k-immu.jp/ja/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.k-immu.jp/ja/index.html

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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