Clarification of mechanism underlying Th2-dominat immune response based on cells migrated from sensitized skin

• Project/Area Number: 22590442
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: Kyoto University (2011-2012); The University of Tokyo (2010)
• Principal Investigator: TOMURA Michio 京都大学, 医学研究科, 准教授 (30314321)
• Co-Investigator(Renkei-kenkyūsha): KABASHIMA Kenji 京都大学, 医学部, 准教授 (00362484) ; KANAGAWA Osam 明石市民病院 (70391919)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: アレルギー / 免疫関連疾患 / 免疫学 / 皮膚炎 / カエデマウス

Research Abstract

In order to understand the mechanism underlying establishment of Th2-dominat immune response in allergic skin dermatitis, we attempted to determine spatiotemporal regulation of movement and antigen-carry of dendritic cell subsets migrated from skin to the draining lymph node. We found that particularly CD103-dermal dendritic cells replaced rapidly in skin and lymph node, and were responsible for enhanced continuous migration for more than 3 days and exogenous protein antigen carry after mechanical stress of skin.

Research Products

• [Presentation] Visualization of skin-derived DC dynamics by photoconvertible protein "KikGR" mice. (2013)
  • Author(s): Michio Tomura, Akihiro Hata, Kenji Kabashima, Osami Kanagawa
  • Organizer: 23th 日本樹状細胞研究会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2013-05-17
• [Presentation] 新規機能可視化蛍光タンパク質を用いた免疫応答の可視化 (2013)
  • Author(s): 戸村 道夫
  • Organizer: 千里ライフサイエンスセミナー D4 細胞の"こころ"を生きた個体で観察する----- 蛍光生体イメージングの最前線
  • Place of Presentation: 千里ライフサイエンスセンター、大阪
  • Year and Date: 2013-01-23
• [Presentation] Approach to understand immune system based on spatiotemporal regulation of immune cells in the entire body (2013)
  • Author(s): 戸村 道夫
  • Organizer: 東京慈恵会医科大学, 第38回日本微小循環学会総会
• [Presentation] 新規機能可視化蛍光タンパク質を用いた免疫応答の可視化 (2013)
  • Author(s): (戸村道夫)
  • Organizer: 千里ライフサイエンスセミナー D4 細胞の“こころ”を生きた個体で観 察する- 蛍光生体イメージングの最前線
  • Place of Presentation: 大阪
  • Invited
• [Presentation] Approach to understand immune system based on spatiotemporal regulation of immune cells in the entire body (2013)
  • Author(s): (Michio Tomura), Kenji Kabashima, Osami Kanagawa
  • Organizer: 第38回日本微小循環学会総会
  • Place of Presentation: 東京
  • Invited
• [Presentation] 色変換蛍光タンパク質「カエデ」発現マウスを用いた全身レベルの免疫細胞動態解明による免疫系の理解 (2012)
  • Author(s): 戸村 道夫
  • Organizer: 第36回 日本リンパ学会総会, シンポジウム
  • Place of Presentation: 日本医科大学 橘桜会館、東京
  • Year and Date: 2012-06-29
• [Presentation] 免疫応答と分子イメージング (2012)
  • Author(s): 戸村 道夫
  • Organizer: 日本薬学会第132年会特別シンポジウム
  • Place of Presentation: 北海道大学、札幌
  • Year and Date: 2012-03-30
• [Presentation] 新規機能性蛍光タンパク質による免疫応答の4Dイメージング (2012)
  • Author(s): 戸村 道夫
  • Organizer: 日本薬学会第132年会 日本103-真皮樹状細胞!リンパ節!103+真皮樹状細胞!Slow! Rapid! 定常時の移行 & 細胞死! Slow!表皮!真皮!ランゲルハンス細胞!Accelerated!Accelerated!A647標識卵白アルブミン!薬科機器協会ワークショップ
  • Place of Presentation: 北海道大学、札幌
  • Year and Date: 2012-03-30
• [Presentation] スペクトル型セルアナライザーを用いた次世代免疫研究最前線 (2012)
  • Author(s): 戸村 道夫
  • Organizer: 第41回日本免疫学会、ランチョンセミナー
  • Place of Presentation: 神戸国際会議場、神戸
• [Presentation] Rapid replacement by apoptosis in the draining lymph nodes sustains dynamics of skin-migratory dendritic cells in the steady state (2012)
  • Author(s): Michio Tomura, Kenji Kabashima, Osami Kanagawa
  • Organizer: 99th Annual meeting of the American association of immunologist.
  • Place of Presentation: Hynes Convension Center, Boston, USA
• [Presentation] Rapid replacement by apoptosis in the draining LN sustains dynamics of skin-migratory dendritic cells in steady state. (2012)
  • Author(s): Michio Tomura, Kenji Kabashima, Osami Kanagawa.
  • Organizer: 3rd Synthetic Immunology
  • Place of Presentation: 京都大学芝蘭会館
• [Presentation] Rapid replacement by apoptosis in the draining lymph nodes sustains dynamics of skin-migratory dendritic cells in the steady state. (2012)
  • Author(s): (Michio Tomura), Kenji Kabashima, Osami Kanagawa
  • Organizer: 99th Annual meeting of the American association of immunologist
  • Place of Presentation: 米国、ボストン
• [Presentation] Drastic increase of DC movement from immunizesd site and rapid replacement of antigen-carrying DC in the draining lymph node sustain initiation of T cell proliferation (2012)
  • Author(s): (Michio Tomura), Asako Sawano, Atsushi Miyawaki, Osami Kanagawa
  • Organizer: 12th International symposium on dendritic cells
  • Place of Presentation: 韓国、テグ
• [Presentation] Rapid replacement by apoptosis in the draining LN sustains dynamics of skin-migratory dendritic cells in steady state. (2012)
  • Author(s): (Michio Tomura), Kenji Kabashima, Osami Kanagawa
  • Organizer: 3rd Synthetic Immunology
  • Place of Presentation: 京都
• [Presentation] 色変換蛍光タンパク質「カエデ」発現マウスを用いた全身レベルの免疫細胞動態解明による免疫系の理解 (2012)
  • Author(s): (戸村道夫)
  • Organizer: 第36回 日本リンパ学会総会
  • Place of Presentation: 東京
  • Invited
• [Presentation] スペクトル型セルアナライザーを用いた次世代免疫研究最前線 (2012)
  • Author(s): (戸村道夫)
  • Organizer: 第41回日本免疫学会
  • Place of Presentation: 神戸
  • Invited
• [Presentation] Drastic increase of DC movement from immunizesd site and rapid replacement of antigen-carrying DC in the draining lymph node sustain initiation of T cell proliferation (2012)
  • Author(s): (Michio Tomura), Asako Sawano, Atsushi Miyawaki, Osami Kanagawa
  • Organizer: 第41回日本免疫学会
  • Place of Presentation: 神戸
• [Presentation] Monitoring cell migration in skin in vivo, using mice expressing "Kaede" Protein : Implications for barrier research. (2011)
  • Author(s): Michio Tomura
  • Organizer: Gordon Research Conferences : Barrier Function of Mammalian Skin
  • Place of Presentation: Waterville Valley Resort, New Hampshire, USA
• [Presentation] 免疫応答の可視化に向けて:4Dイメージングの試み (2011)
  • Author(s): 戸村 道夫
  • Organizer: 日光シンポジウム
  • Place of Presentation: 日光東照宮晃陽苑
• [Presentation] Dynamic movement ofdendritic cells under physiological condition. (2011)
  • Author(s): Michio Tomura, Kenji Kabashima, Osami Kanagawa
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ、千葉
• [Remarks]
  • URL: http://www.ak.med.kyoto-u.ac.jp/group_research/tomuraG.html