| Project/Area Number |
22590507
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied pharmacology
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAMAOKI Tomoko 兵庫医科大学, 医学部, 教授 (10172868)
MORINAGA Tomonori 兵庫医科大学, 医学部, 助教 (10351818)
KITANO Yukio 兵庫医科大学, 医学部, 名誉教授 (70028538)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | ヒト組織利用研究 / オーダーメイド医療 / アトピー性皮膚炎 / 再生医学 / 細胞内シグナル伝達 |
|---|
| Research Abstract |
We cultured human hair follicle-derived keratinocytes (FDKs) from plucked hairs. FDKs may be an ideal cell source for personalized medicine, since they can be established without invasive biopsies. We undertook a comparative study of gene expression in FDKs from adult donors with atopic dermatitis (AD-FDKs) and non-atopic donors (Non-AD-FDKs). AD-FDKs showed higher expression of NLRP2, which encodes a component of inflammasome, and lower expression of DKK1, which encodes an inhibitor of Wnt signalling, than Non-AD-FDKs. Treatment with IFN-γ for 24 hours induced the enhanced expression of IL32, IL1B, IL8, and CXCL1 in AD-FDKs compared to Non-AD-FDKs, showing increased IFN-γ responses in AD-FDKs.
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