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Basic research to enable personalized medicine using follicular bulge-derived stem cells

Research Project

Project/Area Number 22590507
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied pharmacology
Research InstitutionHyogo Medical University

Principal Investigator

YOSHIKAWA Yoshie  兵庫医科大学, 医学部, 助教 (10566673)

Co-Investigator(Kenkyū-buntansha) TAMAOKI Tomoko  兵庫医科大学, 医学部, 教授 (10172868)
MORINAGA Tomonori  兵庫医科大学, 医学部, 助教 (10351818)
KITANO Yukio  兵庫医科大学, 医学部, 名誉教授 (70028538)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsヒト組織利用研究 / オーダーメイド医療 / アトピー性皮膚炎 / 再生医学 / 細胞内シグナル伝達
Research Abstract

We cultured human hair follicle-derived keratinocytes (FDKs) from plucked hairs. FDKs may be an ideal cell source for personalized medicine, since they can be established without invasive biopsies. We undertook a comparative study of gene expression in FDKs from adult donors with atopic dermatitis (AD-FDKs) and non-atopic donors (Non-AD-FDKs). AD-FDKs showed higher expression of NLRP2, which encodes a component of inflammasome, and lower expression of DKK1, which encodes an inhibitor of Wnt signalling, than Non-AD-FDKs. Treatment with IFN-γ for 24 hours induced the enhanced expression of IL32, IL1B, IL8, and CXCL1 in AD-FDKs compared to Non-AD-FDKs, showing increased IFN-γ responses in AD-FDKs.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (4 results)

All 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (2 results)

  • [Journal Article] Transcriptional Analysis of Hair Follicle-Derived Keratinocytes from Donors with AtopicDermatitis Reveals Enhanced Induction of IL32 Gene by IFN-γ.2013

    • Author(s)
      Yoshikawa Y, Sasahara Y, Takeuchi K, Tsujimoto Y, Hashida-Okado T, Kitano Y, Hashimoto-Tamaoki T.
    • Journal Title

      Int J Mol Sci.

      Volume: 14 Issue: 2 Pages: 3215-27

    • DOI

      10.3390/ijms14023215

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transcriptional Analysis of Hair Follicle-Derived Keratinocytes from Donors with Atopic Dermatitis Reveals Enhanced Induction of IL32 Gene by IFN-γ2013

    • Author(s)
      Yoshie Yoshikawa
    • Journal Title

      International Journal of Molecular Science

      Volume: 14 Pages: 3215-3227

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] アトピー性皮膚炎罹患ドナーから培養したバルジ由来ケラチノサイトはIFN-γ刺激によって高いIL32産生能を有する2012

    • Author(s)
      吉川良恵
    • Organizer
      日本分子生物学会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-13
    • Related Report
      2012 Final Research Report
  • [Presentation] アトピー性皮膚炎罹患ドナーから培養したバルジ由来ケラチノサイトはIFN-γ刺激によって高いIL32産生能を有する2012

    • Author(s)
      吉川良恵
    • Organizer
      日本分子生物学会
    • Place of Presentation
      マリンメッセ福岡
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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