| Project/Area Number |
22590514
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YANO Hisakazu 東北大学, 大学院・医学系研究科, 講師 (20374944)
ARAI Kazuaki 東北大学, 大学院・医学系研究科, 研究補佐 (30547386)
HIRAKATA Yoichi 東北大学, 大学院・医学系研究科, 教授 (50238341)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | A(H1N1)pdm09 / community-acquired MRSA / invasion / 新型インフルエンザ / 市中感染型MRSA / 細胞侵入性 / MRSA / 細胞接着能 |
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| Research Abstract |
In this study, we investigated whether prior infection with pandemic A(H1N1)2009 virus (A(H1N1)pdm09) led to increased invasion of airway epithelial cells by CA-MRSA. We performed the gentamicin killing assay using Madin-Darby canine kidney (MDCK) cells and various bacteria to investigate whether prior A(H1N1)pdm09 infection led to increased invasion of these epithelial cells by CA-MRSA. We found that the invasion rate of two MRSA strains (ATCC BAA-1680 (USA 300) and ATCC BAA-1699 (USA 100)) into intact MDCK cell monolayers was 0.29±0.15% and 0.007±0.002%, respectively (p=3). In addition, the relative invasion rate of both ATCC BAA-1680 and ATCC BAA-1699 was significantly increased by prior A(H1N1)pdm09 infection of MDCK monolayers from 1±0.28 to 1.38±0.02 and from 1±0.24 to 1.73±0.29, respectively (p
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