Sudden coronary death, pokkuri disease, and lipoprotein-associated sphingosine 1-phosphate

• Project/Area Number: 22590517
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Gunma University
• Principal Investigator: NAKAJIMA Katsuyuki 群馬大学, 大学院・保健学研究科, 客員教授 (10444051)
• Co-Investigator(Kenkyū-buntansha): FUJITA Masaki 慶應義塾大学, 医学部, 教授 (00211524) ; OKAJIMA Fumikazu 群馬大学, 生体調節研究所, 教授 (30142748)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: スフィンゴシン 1-リン酸 / ポックリ病 / レムナント / 心臓性突然死 / スフィンゴシン1-リン酸 / リポタンパク二次元マップ / apoM / apoA-I / スフィンゴシン1-リン酸(S-1-P) / レムナントリポ蛋白質 / 2次元電気泳動マップ / S1P受容体

Research Abstract

We have proposed that triglyceride-rich lipoprotein remnants are associated with the risk of sudden coronary death in younger cases without coronary atherosclerosis. However, it remains unclear how remnants are involved in coronary sudden death. In the present study, we tried to relate sphingosine1-phosphate (S1P), which is concentrated in lipoproteins, and apolipoprotein M, an S1P binding apolipoprotein, to sudden death. Our new assay method to detect S1P allowed to measure a low concentration of S1P in serum; however, there are any significant difference in S1P levels between normal and sudden death patients. We are now focusing on the difference in two dimensionalelectrophoresis pattern measured by anti-apoA-I and anti-apoM antidodies.

Research Products

• [Journal Article] Comparative reactivity of remnant-like lipoprotein particles (RLP) and low-density lipoprotein (LDL) to LDL receptor and VLDL receptor: effect of a high-dose statin on VLDL receptor expression (2012)
  • Author(s): Imagawa M, Takahashi S, Zenimaru Y, Kimura T, Suzuki J, Miyamori I, Iwasaki T, Hattori H, Yamamoto TT, Nakano T, Nakajima K.
  • Journal Title: Clin Chim Acta Volume: 413 Pages: 441-7
  • Peer Reviewed
• [Journal Article] The characteristics of remnant lipoproteins in the fasting and postprandial plasma (2012)
  • Author(s): Nakajima K, Nakano T, Tokita Y, Nagamine T, Yatsuzuka S, Shimomura Y, Tanaka A, Sumino H, Nara M, Machida T, Murakami M.
  • Journal Title: Clin Chim Acta Volume: 413 Pages: 1077-86
  • Peer Reviewed
• [Journal Article] Remnant lipoproteins are a stronger risk factor for cardiovascular events than LDL-C from the studies of autopsies in sudden cardiac death cases.Recent Advances in Cardiovascular Risk Factors (2012)
  • Author(s): Nakajima K and Fujita MQ.
  • Journal Title: Ed by Mehnaz Atiq. (Intech) Pages: 15-36
• [Journal Article] Post-prandial remnant lipoprotein metabolism in autosomal recessive hypercholesterolaemia. (2012)
  • Author(s): Tada H, Kawashiri MA, Tanaka A, Nakano T, Nakajima K, Inoue T, Noguchi T, Nakanishi C, Konno T, Hayashi K, Nohara A, Inazu A, Kobayashi J, Mabuchi H, Yamagishi M.
  • Journal Title: Eur JClin Invest. Volume: 42 Pages: 1094-9
  • Peer Reviewed
• [Journal Article] ELISA system for human endothelial lipase. (2012)
  • Author(s): Ishida T, Miyashita K, Shimizu M, Kinoshita N, Mori K, Sun L, Yasuda T, Imamura S, Nakajima K, Stanhope KL, Havel PJ, Hirata K.
  • Journal Title: ClinChem Volume: 58 Pages: 1656-64
  • Peer Reviewed
• [Journal Article] Advances in Clinical Chemistry (Review) (2012)
  • Author(s): Nakajima K, Nagamine T, Fijita MQ, Tanaka A. ApoB48
  • Journal Title: submitted
  • Peer Reviewed
• [Journal Article] Comparative reactivity of remnant-like lipoprotein particles (RLP) and low-density lipoprotein (LDL) to LDL receptor and VLDL receptor: effect of a high-dose statin on VLDL receptor expression (2012)
  • Author(s): Imagawa M
  • Journal Title: Clin Chim Acta Volume: 413 Issue: 3-4 Pages: 441-447
  • DOI: 10.1016/j.cca.2011.10.033
  • Peer Reviewed
• [Journal Article] The characteristics of remnant lipoproteins in the fasting and postprandial plasma (2012)
  • Author(s): Nakajima K
  • Journal Title: Clin Chim Acta Volume: 413 Issue: 13-14 Pages: 1077-1086
  • DOI: 10.1016/j.cca.2012.02.026
  • Peer Reviewed
• [Journal Article] Post-prandial remnant lipoprotein metabolism in autosomal recessive hypercholesterolaemia (2012)
  • Author(s): Tada H
  • Journal Title: Eur J Clin Invest Volume: 42 Issue: 10 Pages: 1094-1099
  • DOI: 10.1111/j.1365-2362.2012.02700.x
  • Peer Reviewed
• [Journal Article] ELISA system for human endothelial lipase. (2012)
  • Author(s): Ishida T
  • Journal Title: Clin Chem Volume: 58 Issue: 12 Pages: 1656-1664
  • DOI: 10.1373/clinchem.2012.187914
  • Peer Reviewed
• [Journal Article] Effects of maximal atorvastatin and rosuvastatin treatment on markers of glucose homeostasis and inflammation. (2011)
  • Author(s): Thongtang N, Ai M, Otokozawa S, Himbergen TV, Asztalos BF, Nakajima K, Stein E, Jones PH, Schaefer EJ.
  • Journal Title: Am J Cardiol Volume: 107 Pages: 387-92
  • Peer Reviewed
• [Journal Article] Adiponectin: an independent risk factor for coronary heart disease in men in the Framingham offspring Study (2011)
  • Author(s): Ai M, Otokozawa S, Asztalos BF, White CC, Cupples LA, Nakajima K, Lamon-Fava S, Wilson PW, Matsuzawa Y, Schaefer EJ
  • Journal Title: Atherosclerosis Volume: 217 Pages: 543-8
  • Peer Reviewed
• [Journal Article] Measurement of serum remnant-like lipoprotein particle-triglyceride (RLP-TG) and RLP-TG/ total TG ratio using highly sensitive triglyceride assayreagent (2011)
  • Author(s): Nakano T, Tokita Y, Nagamine T, Tanaka A, Okazaki M, Yatsuzuka S, Tarui N, Shimomura Y, Ando Y, Akamatsu S, Fujita MQ, Nakajima K
  • Journal Title: Clin Chim Acta Volume: 12 Pages: 71-78
  • Peer Reviewed
• [Journal Article] Particle size of apoB-48 carrying lipoproteins in remnant lipoproteins isolated from postprandial plasma (2011)
  • Author(s): Nakano T, Tanaka A, Okazaki M, Tokita Y, Nagamine T, Nakajima K.
  • Journal Title: Ann Clin Biochem Volume: 48 Pages: 57-64
  • Peer Reviewed
• [Journal Article] Postprandial lipoprotein metabolism: VLDL vs chylomicrons (2011)
  • Author(s): Nakajima K, Nakano T, Tokita Y, Nagamine T, Inazu A, Kobayashi J, Mabuchi H, Stanhope KL, Havel PJ, Okazaki M, Ai M, Tanaka A.
  • Journal Title: Clin Chim Acta Volume: 412 Pages: 1306-1318
  • Peer Reviewed
• [Journal Article] Consumption of fructose and high fructose corn syrup increase postprandial triglycerides, LDL-cholesterol, and apolipoprotein-B in young men and women. (2011)
  • Author(s): Stanhope KL, Bremer AA, Medici V, Nakajima K, Ito Y, Nakano T, Chen G, Fong TH, Lee V, Menorca RI, Keim NL, Havel PJ.
  • Journal Title: J Clin Endocrinol Metab. Volume: 96 Pages: 3783-91
  • Peer Reviewed
• [Journal Article] Metabolic responses to prolonged consumption of glucose- and fructose-sweetened beverages are not associated with postprandial or 24-h glucose and insulin excursions (2011)
  • Author(s): Stanhope KL, Griffen SC, Bremer AA, Vink RG, Schaefer EJ, Nakajima K, Schwarz JM, Beysen C, Berglund L, Keim NL, Havel PJ.
  • Journal Title: Am J Clin Nutr Volume: 94 Pages: 112-9
  • Peer Reviewed
• [Journal Article] Non-HDL cholesterol shows improved accuracy for cardiovascular risk score classification compared to direct or calculated LDL cholesterol in a dyslipidemic population (2011)
  • Author(s): van Deventer HE, Miller WG, Myers GL, Sakurabayashi I, Bachmann LM, Caudill SP,Dziekonski A, Edwards S, Kimberly MM, Korzun WJ, Leary ET, Nakajima K, Nakamura M, Shamburek RD, Vetrovec GW, Warnick GR, Remaley AT
  • Journal Title: Clin Chem Volume: 57 Pages: 490-501
  • Peer Reviewed
• [Journal Article] Measurement of serum remnant-like lipoprotein particle-triglyceride (RLP-TG) and RLP-TG/total TG ratio using highly sensitive triglyceride assay reagent (2011)
  • Author(s): Nakajima K, Fujita MQ
  • Journal Title: Clinica Chimca Acta Volume: 412 Pages: 71-78
  • Peer Reviewed
• [Journal Article] Postprandial lipoprotein metabolism : VLDL vs chylomicrons (2011)
  • Author(s): Nakajima K
  • Journal Title: Clinica Chimca Acta Volume: 412 Pages: 1306-1318
• [Journal Article] Unmasking of LPA(1) receptor-mediated migration response to lysophosphatidic acid by interleukin-1β-induced attenuation of Rho signaling pathways in rat astrocytes (2011)
  • Author(s): Sato K, Okajima F
  • Journal Title: J Neurochem Volume: 117 Pages: 164-174
  • Peer Reviewed
• [Journal Article] Pokkuri Death Syndrome ; Sudden Cardiac Death Cases without Coronary Atherosclerosis in South Asian Young Males. (2011)
  • Author(s): Nakajima K, Takeichi S, Nakajima Y, Fujita MQ.
  • Journal Title: Forensic Science International Volume: 207 Pages: 6-13
  • Peer Reviewed
• [Journal Article] Association of angiopoietin-like protein 3 with hepatic triglyceride lipase and lipoprotein lipase activities in human plasma (2010)
  • Author(s): Nakajima K, Kobayashi J, Mabuchi H, Nakano T, Tokita Y, Nagamine T, Imamura S, Ai M, Otokozawa S, Schaefer EF
  • Journal Title: Ann Clin Biochem Volume: 47 Pages: 423-31
  • NAID: 120002519245
  • Peer Reviewed
• [Journal Article] Direct assessment of plasma low density lipoprotein and high density lipoprotein cholesterol levels and coronary heart disease: Results from the Framingham Offspring Study (2010)
  • Author(s): Otokozawa S, Ai M, Asztalos BF, White CC, Demissie-Banjaw S, Cupples LA, Nakajima K, Wilson PW, Schaefer EJ.
  • Journal Title: Atherosclerosis Volume: 213 Pages: 251-5
  • Peer Reviewed
• [Journal Article] Remaley ATSeven direct methods for measuring HDL and LDL cholesterol compared with ultracentrifugation reference measurement procedures (2010)
  • Author(s): Miller WG, Myers GL, Sakurabayashi I, Bachmann LM, Caudill SP, Dziekonski A, Edwards S, Kimberly MM, Korzun WJ, Leary ET, Nakajima K, Nakamura M, Nilsson G, Shamburek RD, Vetrovec GW, Warnick GR
  • Journal Title: Clin Chem. Volume: 56(6) Pages: 977-86
  • Peer Reviewed
• [Journal Article] Small dense LDL cholesterol and coronary heart disease: results from the Framingham Offspring Study (2010)
  • Author(s): Ai M, Otokozawa S, Asztalos BF, Ito Y,Nakajima K, White CC, Cupples LA, Wilson PW, Schaefer EJ.
  • Journal Title: Clin Chem Volume: 56 Pages: 967-76
  • Peer Reviewed
• [Journal Article] Pokkuri Death Syndrome; Sudden Cardiac Death Cases without Coronary Atherosclerosis in South Asian Young Males. (2010)
  • Author(s): Nakajima K, Takeichi S, Nakajima Y, Fujita MQ.
  • Journal Title: Forensic Sci Int Volume: 207 Pages: 6-13
  • Peer Reviewed
• [Journal Article] 心臓性突然死"タイのりLai-Tai"と"日本のポックリ病"の比較研究 (2010)
  • Author(s): 藤田眞幸、中嶋克行
  • Journal Title: 医学のあゆみ Volume: Vol.235 No.7(依頼原稿) Pages: 797-799
• [Journal Article] Role of sphingosine 1-phosphate in anti-atherogenic actions of high-density lipoprotein (2010)
  • Author(s): Sato K, Okajima F.1 : (2010) (Invited review)
  • Journal Title: World J Biol Chem Volume: 1(依頼原稿) Pages: 327-337
• [Presentation] Panuwat Chutivongse, Nantana Sirisup, Sanae Takeichi, Masaki Q Fujita: The relationship between the severity of coronary artery atherosclerosis and heart weight in Asian sudden death cases (2012)
  • Author(s): Katsuyuki Nakajima Yasuhiro Nakajima
  • Organizer: The XVI International Symposium on Atherosclerosis (ISA2012)
  • Place of Presentation: Sydney Australia
• [Presentation] Ernst Schaefer Katsuyuki Nakajima: Plasma diagnostic markers of fatty liver in subjects with or without metabolic syndrome (2012)
  • Author(s): Shinichi Yatsuzuka, Masako Akuzawa, Masaki Takahashi, Younosuke Shimomura, Yoshitaka Ando, Isao Kobayashi, Takamitsu Nakano, Yoshiharu Tokita, Takeaki Nagamine, Hiromi Ono, Akira Tanaka
  • Organizer: The XVI International Symposium on Atherosclerosis (ISA2012)
  • Place of Presentation: Sydney Australia
• [Presentation] Effectof very-low-density lipoprotein (VLDL) Receptor on plasma triglyceride and the increased VLDL receptor expression by high dose statin, low fat and low glucose (2012)
  • Author(s): Michiko Imagawa, Sadao Takahashi1, Yasuo Zenimaru, Mai Ichikawa, Tomoko Kimura, Satsuki Sato, Jinya Suzuki, Isamu Miyamori, Takamitsu Nakano, Katsuyuki Nakajima
  • Organizer: The XVI International Symposium on Atherosclerosis (ISA2012)
  • Place of Presentation: Sydney Australia
• [Presentation] The characteristics of remnant lipoproteins in the fasting and postprandial plasma. (2012)
  • Author(s): Nakajima K, Nakano T, Y. Tokita, T. Nagamine, Yatsuzuka S, Shimomura Y, Tanaka A, Sumino H, Nara M, Machida T, Murakami M.
  • Organizer: 3rd International Symposium on Chylomicrons in Disease
  • Place of Presentation: Sydney, Australia
• [Presentation] The relationship between the severity of coronary artery atherosclerosis and heart weight in Asian sudden death cases (2012)
  • Author(s): Nakajima K, Fujita MQ
  • Organizer: The XVI International Symposium on Athero (sclerosis ISA2012
  • Place of Presentation: オーストラリア、シドニー
• [Presentation] Measurement of plasma remnant lipoproteins and its clinical significance (2011)
  • Author(s): Katsuyuki Nakajima
  • Organizer: American Association of Clinical Chemistry, Lipoproteins and Vascular Disease Division
  • Place of Presentation: Atlanta, Georgia, USA
  • Year and Date: 2011-07-25
• [Presentation] The presence of postprandial remnant lipoproteins in the fasting plasma with TG levels below 150 mg/dL (2011)
  • Author(s): Nakajima K, Nakano T, Tokita Y, Nagamne T, Yatsuzuka S, Shimomura Y. Tanaka
  • Organizer: American Association of Clinical Chemistry, Lipoproteins and Vascular Disease Division
  • Place of Presentation: Atlanta, Georgia, USA
• [Presentation] Characteristics of apolB48 and apoB100 carrying remnant lipoproteins (RLP) in postprandial plasma. (2010)
  • Author(s): Katsuyuki Nakajima
  • Organizer: International Symposium on Chylomicrons in Disease-2
  • Place of Presentation: オランダ、ロッテルダム市'
• [Book] Recent Advances in Cardiovascular Risk Factors (2012)
  • Author(s): Nakajima K
  • Total Pages: 22
  • Publisher: Intech
• [Book] Recent Advances in Cardiovascular Risk Factors (2012)
  • Author(s): Nakajima K, Fujita MQ
  • Publisher: Remnant Lipoproteins are a Stronger Risk Factor for Cardiovascular Events than LDL-C-From the Studies of Autopsies in Sudden Cardiac Death Cases