Development of rapid test for biomarkers in exhaled breath condensate in patients with asthma and its use for the management of asthmatics
| Project/Area Number |
22590526
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Okayama University |
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Principal Investigator |
KATAOKA Mikio 岡山大学, 大学医保健学研究科, 教授 (50177391)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBAKURA Misako 岡山大学, 大学医保健学研究科, 准教授 (30314694)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 呼気凝縮液 / 気管支喘息 / 免疫クロマト法 |
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| Research Abstract |
To evaluate airway inflammation in asthmatic patients, we developed a rapid test as point of care testing detecting neutrophilic and eosinophilic biomarkers in exhaled breath condensate (EBC). Major basic protein (MBP) for eosinophilic marker and Myeloperoxidase (MPO) for neutrophilic marker were detected in EBC by ELISA methods in patients with asthma. We established immunochromatography for semi-quantification of MPO and MBP in EBC. The findings of immunochromatography indicate that asthmatic patients were divided into two groups including eosinophilic marker predominant group and neutrophilic marker predominant group. This method could be applied to the management of asthma.
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Report
(4 results)
Research Products
(15 results)
