Development of rapid test for biomarkers in exhaled breath condensate in patients with asthma and its use for the management of asthmatics

• Project/Area Number: 22590526
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Okayama University
• Principal Investigator: KATAOKA Mikio 岡山大学, 大学医保健学研究科, 教授 (50177391)
• Co-Investigator(Kenkyū-buntansha): SHIBAKURA Misako 岡山大学, 大学医保健学研究科, 准教授 (30314694)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 呼気凝縮液 / 気管支喘息 / 免疫クロマト法

Research Abstract

To evaluate airway inflammation in asthmatic patients, we developed a rapid test as point of care testing detecting neutrophilic and eosinophilic biomarkers in exhaled breath condensate (EBC). Major basic protein (MBP) for eosinophilic marker and Myeloperoxidase (MPO) for neutrophilic marker were detected in EBC by ELISA methods in patients with asthma. We established immunochromatography for semi-quantification of MPO and MBP in EBC. The findings of immunochromatography indicate that asthmatic patients were divided into two groups including eosinophilic marker predominant group and neutrophilic marker predominant group. This method could be applied to the management of asthma.

Research Products

• [Journal Article] Inhibition of neutrophil elastase attenuates airway hyperresponsiveness and inflammation in a mouse model of secondary allergen challenge:neutrophil elastase inhibition attenuates allergic airway response. (2013)
  • Author(s): Hikari Koga, Nobuaki Miyahara, Yasuko Fuchimoto, Mikio Kataoka(他7名)
  • Journal Title: Respiratory Research Volume: 14 Pages: 8-8
  • Peer Reviewed
• [Journal Article] IL-17A is essential to the development of elastase-induced pulmonary inflammation and emphysema in mice. (2013)
  • Author(s): Etsuko Kurimoto, Nobuaki Miyahara, Arihiko Kanehiro, Mikio Kataoka(他8名)
  • Journal Title: Respiratory Research Volume: 14 Pages: 5-5
  • NAID: 120005425640
  • Peer Reviewed
• [Journal Article] Inhibition of neutrophil elastase attenuates airway hyperresponsiveness and inflammation in a mouse model of secondary allergen challenge: neutrophil elastase inhibition attenuates allergic airway response (2013)
  • Author(s): Hikari Koga
  • Journal Title: Respiratory Research Volume: 14 Issue: 1 Pages: 8-8
  • DOI: 10.1186/1465-9921-14-8
  • Peer Reviewed
• [Journal Article] IL-17A is essential to the development of elastase-induced pulmonary inflammation and emphysema in mice (2013)
  • Author(s): Etsuko Kurimoto
  • Journal Title: Respiratory Research Volume: 14 Issue: 1 Pages: 5-5
  • DOI: 10.1186/1465-9921-14-5
  • Peer Reviewed
• [Journal Article] Blocking the leukotriene B4 receptor 1 inhibits late-phase airway responses in established disease. (2011)
  • Author(s): Waseda Koichi, Miyahara Nobuaki, Kanehiro Arihiko, Kataoka Mikio
  • Journal Title: Am J Respir Cell Mol Biol Volume: 45 Pages: 851-857
  • Peer Reviewed
• [Journal Article] Requirement for chemokine receptor 5 in the development of allergen-induced airway hyperresponsiveness and inflammation. (2011)
  • Author(s): Yasuko Fuchimoto, Arihiko Kanehiro, Nobuaki Miyahara, Mikio Kataoka
  • Journal Title: Am J Respir Cell Mol Biol Volume: 45 Pages: 1248-1255
  • Peer Reviewed
• [Journal Article] Requirement for Chemokine Receptor 5 in the Development of Allergen-Induced Airwavy Hyperresponsiveness and Inflammation (2011)
  • Author(s): Yasuko Fuchimoto
  • Journal Title: Am.J.Respir.Cell Mol.Biol. Volume: 45 Pages: 1248-1255
  • Peer Reviewed
• [Journal Article] Blocking the Leukotriene B4 Receptor 1 Inhibits Late-Phase Airway Responses in Established Disease (2011)
  • Author(s): Koichi Waseda
  • Journal Title: Am.J.Respir.Cell Mol.Biol. Volume: 45 Pages: 851-857
  • Peer Reviewed
• [Journal Article] 原発性胆汁性肝硬変を合併したサルコイドーシスの3例 (2010)
  • Author(s): 能島大輔、谷本 安、栗本悦子、片岡幹男(他7名)
  • Journal Title: 日本サルコイドーシス/肉芽腫性疾患学会誌 Volume: 30 Pages: 27-32
  • NAID: 130001930665
  • Peer Reviewed
• [Journal Article] 呼吸器疾患 ～肺炎を中心に～ (2010)
  • Author(s): 片岡幹男
  • Journal Title: 感染防止 Volume: 20 Pages: 56-68
  • Peer Reviewed
• [Journal Article] Experimental pulmonary granuloma mimicking sarcoidosis induced by Propionibacterium acnes in mice. (2010)
  • Author(s): Kouji Iio, Tomoe Ueno Iio, Mikio Kataoka(他6名目)
  • Journal Title: Acta Med Okayama Volume: 64 Pages: 75-83
  • NAID: 120002311479
  • Peer Reviewed
• [Journal Article] Experimental pulmonary granuloma mimicking sarcoidosis induced by Propionibacterium acnes in mice (2010)
  • Author(s): Kouji Iio, Tomoe Ueno Iio, Mikio Kataoka, et al
  • Journal Title: Acta Med Okayama Volume: 64 Pages: 75-83
  • NAID: 120002311479
  • Peer Reviewed
• [Journal Article] 呼吸器疾患～肺炎を中心に～ (2010)
  • Author(s): 片岡幹男
  • Journal Title: 感染防止 Volume: 20 Pages: 56-68
• [Presentation] 呼気凝縮液(EBC)による気管支喘息患者の気道炎症の診断 (2011)
  • Author(s): 兵田朋子
  • Organizer: 日本臨床検査医学会
  • Place of Presentation: 岡山市
  • Year and Date: 2011-11-19
• [Presentation] ステロイド薬減量中に再燃を繰り返す慢性好酸球性肺炎にシクロフォスファミドの併用が有効であった1例 (2010)
  • Author(s): 能島大輔
  • Organizer: 第22回日本アレルギー学会春季臨床大会
  • Place of Presentation: 京都
  • Year and Date: 2010-05-08