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Development of rapid test for biomarkers in exhaled breath condensate in patients with asthma and its use for the management of asthmatics

Research Project

Project/Area Number 22590526
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionOkayama University

Principal Investigator

KATAOKA Mikio  岡山大学, 大学医保健学研究科, 教授 (50177391)

Co-Investigator(Kenkyū-buntansha) SHIBAKURA Misako  岡山大学, 大学医保健学研究科, 准教授 (30314694)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords呼気凝縮液 / 気管支喘息 / 免疫クロマト法
Research Abstract

To evaluate airway inflammation in asthmatic patients, we developed a rapid test as point of care testing detecting neutrophilic and eosinophilic biomarkers in exhaled breath condensate (EBC). Major basic protein (MBP) for eosinophilic marker and Myeloperoxidase (MPO) for neutrophilic marker were detected in EBC by ELISA methods in patients with asthma. We established immunochromatography for semi-quantification of MPO and MBP in EBC. The findings of immunochromatography indicate that asthmatic patients were divided into two groups including eosinophilic marker predominant group and neutrophilic marker predominant group. This method could be applied to the management of asthma.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (15 results)

All 2013 2011 2010

All Journal Article (13 results) (of which Peer Reviewed: 12 results) Presentation (2 results)

  • [Journal Article] Inhibition of neutrophil elastase attenuates airway hyperresponsiveness and inflammation in a mouse model of secondary allergen challenge:neutrophil elastase inhibition attenuates allergic airway response.2013

    • Author(s)
      Hikari Koga, Nobuaki Miyahara, Yasuko Fuchimoto, Mikio Kataoka(他7名)
    • Journal Title

      Respiratory Research

      Volume: 14 Pages: 8-8

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] IL-17A is essential to the development of elastase-induced pulmonary inflammation and emphysema in mice.2013

    • Author(s)
      Etsuko Kurimoto, Nobuaki Miyahara, Arihiko Kanehiro, Mikio Kataoka(他8名)
    • Journal Title

      Respiratory Research

      Volume: 14 Pages: 5-5

    • NAID

      120005425640

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibition of neutrophil elastase attenuates airway hyperresponsiveness and inflammation in a mouse model of secondary allergen challenge: neutrophil elastase inhibition attenuates allergic airway response2013

    • Author(s)
      Hikari Koga
    • Journal Title

      Respiratory Research

      Volume: 14 Issue: 1 Pages: 8-8

    • DOI

      10.1186/1465-9921-14-8

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] IL-17A is essential to the development of elastase-induced pulmonary inflammation and emphysema in mice2013

    • Author(s)
      Etsuko Kurimoto
    • Journal Title

      Respiratory Research

      Volume: 14 Issue: 1 Pages: 5-5

    • DOI

      10.1186/1465-9921-14-5

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Blocking the leukotriene B4 receptor 1 inhibits late-phase airway responses in established disease.2011

    • Author(s)
      Waseda Koichi, Miyahara Nobuaki, Kanehiro Arihiko, Kataoka Mikio
    • Journal Title

      Am J Respir Cell Mol Biol

      Volume: 45 Pages: 851-857

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Requirement for chemokine receptor 5 in the development of allergen-induced airway hyperresponsiveness and inflammation.2011

    • Author(s)
      Yasuko Fuchimoto, Arihiko Kanehiro, Nobuaki Miyahara, Mikio Kataoka
    • Journal Title

      Am J Respir Cell Mol Biol

      Volume: 45 Pages: 1248-1255

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Requirement for Chemokine Receptor 5 in the Development of Allergen-Induced Airwavy Hyperresponsiveness and Inflammation2011

    • Author(s)
      Yasuko Fuchimoto
    • Journal Title

      Am.J.Respir.Cell Mol.Biol.

      Volume: 45 Pages: 1248-1255

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Blocking the Leukotriene B4 Receptor 1 Inhibits Late-Phase Airway Responses in Established Disease2011

    • Author(s)
      Koichi Waseda
    • Journal Title

      Am.J.Respir.Cell Mol.Biol.

      Volume: 45 Pages: 851-857

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 原発性胆汁性肝硬変を合併したサルコイドーシスの3例2010

    • Author(s)
      能島大輔、谷本 安、栗本悦子、片岡幹男(他7名)
    • Journal Title

      日本サルコイドーシス/肉芽腫性疾患学会誌

      Volume: 30 Pages: 27-32

    • NAID

      130001930665

    • Related Report
      2012 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 呼吸器疾患 ~肺炎を中心に~2010

    • Author(s)
      片岡幹男
    • Journal Title

      感染防止

      Volume: 20 Pages: 56-68

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Experimental pulmonary granuloma mimicking sarcoidosis induced by Propionibacterium acnes in mice.2010

    • Author(s)
      Kouji Iio, Tomoe Ueno Iio, Mikio Kataoka(他6名目)
    • Journal Title

      Acta Med Okayama

      Volume: 64 Pages: 75-83

    • NAID

      120002311479

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Experimental pulmonary granuloma mimicking sarcoidosis induced by Propionibacterium acnes in mice2010

    • Author(s)
      Kouji Iio, Tomoe Ueno Iio, Mikio Kataoka, et al
    • Journal Title

      Acta Med Okayama

      Volume: 64 Pages: 75-83

    • NAID

      120002311479

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 呼吸器疾患~肺炎を中心に~2010

    • Author(s)
      片岡幹男
    • Journal Title

      感染防止

      Volume: 20 Pages: 56-68

    • Related Report
      2010 Annual Research Report
  • [Presentation] 呼気凝縮液(EBC)による気管支喘息患者の気道炎症の診断2011

    • Author(s)
      兵田朋子
    • Organizer
      日本臨床検査医学会
    • Place of Presentation
      岡山市
    • Year and Date
      2011-11-19
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] ステロイド薬減量中に再燃を繰り返す慢性好酸球性肺炎にシクロフォスファミドの併用が有効であった1例2010

    • Author(s)
      能島大輔
    • Organizer
      第22回日本アレルギー学会春季臨床大会
    • Place of Presentation
      京都
    • Year and Date
      2010-05-08
    • Related Report
      2012 Final Research Report 2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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