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Development of bio-assay system of HTLV-1 infection using xenograft model mice

Research Project

Project/Area Number 22590532
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionUniversity of Miyazaki

Principal Investigator

OKAYAMA Akihiko  宮崎大学, 医学部, 教授 (70204047)

Co-Investigator(Kenkyū-buntansha) TAKAJO Ichiro  宮崎大学, 医学部, 講師 (20418841)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsHTLV-1 / ATL / NOGマウス / ゼノグラフトモデル / 感染予防 / 発症予防
Research Abstract

HTLV-1 is a causative agent of adult T-cell leukemia. In the present study, we tried to clarify the mechanism of cell to cell infection and clonal evolution of HTLV-1 infected cells using xenograft model of mice. The HTLV-1 infected cells from asymptomatic carriers survived and proliferated in NOG mice. The HTLV-1 proviral loads were higher when the PBMCs from long-term carriers were inoculated to the mice. Moreover, NOG mice,which received the sera from carriers or neutralizing antibodies, had decreased proviral loads and limited clonal proliferation of HTLV-1 infected cells. These data suggested this mouse model provide the novel information of themechanism of HTLV-1 infection and may be applicable for the risk assessment of ATL development from HTLV-1 carriers.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (27 results)

All 2012 2011 2010

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (11 results)

  • [Journal Article] 特集/ストップザ性感染症 性感染症-診断・治療 HTLV-1 感染2012

    • Author(s)
      岡山昭彦
    • Journal Title

      臨床と研究

      Volume: 89(7) Pages: 907-910

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 日本国内の HTLV-1 感染の現状と今後の対策2012

    • Author(s)
      岡山昭彦
    • Journal Title

      アボットニュース

      Pages: 1-4

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Proviral loads of human T-lymphotropic virus type 1 in asymptomatic carriers with different infection routes2012

    • Author(s)
      S. Ueno K. Umeki I. Takajo Y. Nagatomo N. Kusumoto K. Umekita K. Morishita A. Okayama
    • Journal Title

      Int J Cancer

      Volume: 130(10) Issue: 10 Pages: 2318-2326

    • DOI

      10.1002/ijc.26289

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HTLV-1 感染症:母子感染予防対策とその課題.2012

    • Author(s)
      岡山昭彦
    • Journal Title

      臨床とウィルス

      Volume: 40

    • NAID

      10030572896

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Clinical significance of CADM1/TSLC1/IgSF4 expression in adult T-cell leukemia/lymphoma2012

    • Author(s)
      Nakahata S, Saito Y, Marutsuka K, Hidaka T, Maeda K, Hatakeyama K, Shiraga T, Goto A, Takamatsu N, Asada Y, Utsunomiya A, Okayama A, Kubuki Y, Shimoda K, Ukai Y, Kurosawa G, Morishita K
    • Journal Title

      Leukemia

      Volume: Jan;26 (6) Issue: 6 Pages: 1238-46

    • DOI

      10.1038/leu.2011.379

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 特集/ストップ ザ 性感染症 性感染症―診断・治療 HTLV-1 感染.2012

    • Author(s)
      岡山昭彦
    • Journal Title

      臨床と研究

      Volume: 89(7) Pages: 907-910

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 特集(1):HTLV-1 感染の検査と臨床 2.HTLV-1 感染の疫学.2011

    • Author(s)
      高城一郎, 岡山昭彦
    • Journal Title

      医療と検査機器・試薬 別冊 機器・試薬

      Volume: 34(4) Pages: 447-452

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Defective human T-lymphotropic virus type 1 provirus in asymptomatic carriers2011

    • Author(s)
      Takenouchi H, Umeki K, Sasaki D, I. Yamamoto H. Nomura I. Takajo S. Ueno Umekita K, Kamihira S, Morishita K, A. Okayama
    • Journal Title

      Int J Cancer

      Volume: 128(6) Pages: 1335-1343

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 特集(1):HTLV-1感染の検査と臨床2.HTLV-1感染の疫学2011

    • Author(s)
      高城一郎, 岡山昭彦
    • Journal Title

      医療と検査機器・試薬別冊機器・試薬

      Volume: 34(4) Pages: 447-452

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Defective human T-lymphotropic virus type 1 provirus in asymptomatic carriers.2011

    • Author(s)
      Takenouchi H, Umeki K, Sasaki D, Yamamoto I, Nomura H, Takajo I, Ueno S, Umekita K, Kamihira S, Morishita K, Okayama A.
    • Journal Title

      International Journal of Cancer

      Volume: 128(6) Pages: 1335-1343

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intra- and inter-laboratory variability in human T-cell leukemia virus type-1 proviral load quantification using real-time polymerase chain reaction assays: A multi-center study2010

    • Author(s)
      S. Kamihira Y. Yamano M. Iwanaga D. Sasaki M. Satake A. Okayama K. Umeki R. Kubota S. Izumo K. Yamaguchi T. Watanabe
    • Journal Title

      Cancer Science

      Volume: 101(11) Pages: 2361-2367

    • NAID

      10027833409

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human T-cell Leukemia virus type I (HTLV‐1) proviral load and disease progression in asymptomatic HTLV‐ 1 carriers: a nationwibe prospective study in Japan.2010

    • Author(s)
      Iwanaga M, Watanabe T, Utsunomiya A, A. Okayama K. Uchimaru Ki-Koh. Ryang M. Ogata H. Kikuchi Y. Sagara K. Uozumi M. Mochizuki Tsukasaki K, Saburi Y, Yamamura M, J. Tanaka Y. Moriuchi S. Hino S. Kamihira and K. Yamaguchi, for the Joint Study on Predisposing Factors of ATL Development investigators.
    • Journal Title

      BLOOD

      Volume: 116(8) Pages: 1211-1219

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Downregulation of CDKN1A in Adult T-Cell Leukemia/Lymphoma despite Overexpression of CDKN1A in Human T-Lymphotropic Virus 1-Infected Cell Lines.2010

    • Author(s)
      Watanabe M, Nakahata S, Hamasaki M, Y. Saito Y. Kawano T. Hidaka K. Yamashita K. Umeki T. Taki M. Taniwaki A. Okayama K. Morishita
    • Journal Title

      Journal of Virology

      Volume: 84(14) Pages: 6966-6977

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Downregulation of CDKN1A in Adult T-Cell Leukemia/Lymphoma despite Overexpression of CDKN1A in Human T-Lymphotropic Virus 1-Infected Cell Lines.2010

    • Author(s)
      Watanabe M, Nakahata S, Hamasaki M, Saito Y, Kawano Y, Hidaka T, Yamashita K, Umeki K, Taki T, Taniwaki M, Okayama A, Morishita K.
    • Journal Title

      Journal of Virology.

      Volume: 84(14) Pages: 6966-6977

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Human T-cell Leukemia virus type I (HTLV-1) proviral load and disease progression in asymptomatic HTLV-1 carriers : a nationwibe prospective study in Japan.2010

    • Author(s)
      Iwanaga M, Watanabe T, Utsuno miya A, Okayama A, Uchimaru K, Ki-Ryang Koh, Ogata M, Kikuchi H, Sagara Y, Uozumi K, Mochizuki M, Tsukasaki K, Saburi Y, Yamamura M, Tanaka J, Moriuchi Y, Hino S, Kamihira S, Yamaguchi K, for the Joint Study on Predisposing Factors of ATL Development investigators.
    • Journal Title

      BLOOD

      Volume: 116(8) Pages: 1211-1219

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intra- and inter-laboratory variability in human T-cell leukemia virus type-1 proviral load quantification using real-time polymerase chain reaction assays : A multi-center study.2010

    • Author(s)
      Kamihira S, Yamano Y, Iwanaga M, Sasaki D, Satake M, Okayama A, Umeki K, Kubota R, Izumo S, Yamaguchi K, Watanabe T.
    • Journal Title

      Cancer Science

      Volume: 101(11) Pages: 2361-2367

    • NAID

      10027833409

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 本邦における HTLV-1 感染とキャリア指導の留意点2012

    • Author(s)
      岡山昭彦
    • Organizer
      第36回日本血液事業学会総会共催ランチョンセミナー
    • Place of Presentation
      宮城県仙台市, 仙台国際センター
    • Year and Date
      2012-10-17
    • Related Report
      2012 Final Research Report
  • [Presentation] HTLV-1キャリア末梢血単核球を移植した NOG マウスにおけるHTLV-1プロウイルスDNA のメチル化の動態2012

    • Author(s)
      梅木一美, 山本成郎, 橋倉悠輝, 上野史朗, 高城一郎, 森下和弘, 岡山昭彦
    • Organizer
      第5回 HTLV-1 研究会
    • Place of Presentation
      東京都港区, 東京大学医科学研究所講堂
    • Year and Date
      2012-08-26
    • Related Report
      2012 Final Research Report
  • [Presentation] 抗HTLV-1 抗体陽性関節リウマチ患者の臨床像についての検討2012

    • Author(s)
      押領司大助, 松田基弘, 河野徳明, 姫路大輔, 甲斐泰文, 小野伸之, 上田章
    • Organizer
      第43回九州リウマチ学会
    • Place of Presentation
      大分県大分市, iichiko 総合文化センター
    • Related Report
      2012 Final Research Report
  • [Presentation] HTLV-1キャリア末梢血単核球を移植したNOGマウスにおけるHTLV-1プロウイルスDNAのメチル化の動態.2012

    • Author(s)
      梅木一美, 山本成郎, 橋倉悠輝, 上野史朗, 高城一郎, 森下和弘, 岡山昭彦.
    • Organizer
      第5回HTLV-1研究会
    • Place of Presentation
      東京大学医科学研究所講堂
    • Related Report
      2012 Annual Research Report
  • [Presentation] HTLV-1無症候性キャリアにおける2型欠損プロウイルスの解析2011

    • Author(s)
      梅木一美, 山本成郎, 上野史朗, 高城一郎, 岡山昭彦
    • Organizer
      第58回日本臨床検査医学会学術集会
    • Place of Presentation
      岡山コンベンションセンター(岡山市)
    • Year and Date
      2011-11-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] HTLV-1 無症候性キャリアにおける 2 型欠損プロウィルスの解析2011

    • Author(s)
      梅木一美, 山本成郎, 上野史朗, 高城一郎, 岡山昭彦
    • Organizer
      第58回日本臨床検査医学会学術集会
    • Place of Presentation
      岡山県岡山市, 岡山コンベンションセンター
    • Related Report
      2012 Final Research Report
  • [Presentation] HTLV-1無症候性キャリアの異なる領域のプロウイルス量基づいた感染経路の同定2010

    • Author(s)
      上野史朗, 梅不一美, 野村創, 山本成郎, 竹之内博之, 高城一郎, 岡山昭彦
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪インターナショナルコンベンションセンター(大阪府)
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 無症候性キャリアにおける欠損型HTLV-1プロウイルス2010

    • Author(s)
      梅木一美, 竹ノ内博之, 山本成郎, 岡山昭彦, 佐々木大介, 上平憲
    • Organizer
      第57回日本臨床検査医学会学術集会
    • Place of Presentation
      京王プラザホテル(東京都)
    • Year and Date
      2010-09-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] HTLV-1 proviral load and the relation to disease progression in carriers: a nationwide cohort study (OS-3-147)2010

    • Author(s)
      M. Iwanaga T. Watanabe A. Utsunomiya A. Okayama K. Uchimaru K. Koh M. Ogata H. Kikuchi Y. Sagara K. Uozumi M. Mochizuki K. Tsukasaki Y. Saburi M. Yamamura J. Tanaka Y. Moriuchi S. Hino S. Kamihira K. Yamaguchi (JSPFAD investigators)
    • Organizer
      The 72nd Annual Meeting of the Japanese Society of Hematology
    • Place of Presentation
      Japan Kanagawa-ken Yokohama, PACIFICO YOKOHAMA
    • Related Report
      2012 Final Research Report
  • [Presentation] HTLV-1 無症候性キャリアの異なる領域のプロウイルス量に基づいた感染経路の同定2010

    • Author(s)
      上野史朗, 梅木一美, 野村創, 山本成郎, 竹之内博之, 高城一郎, 岡山昭彦
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪府大阪市, 大阪インターナショナルコンベンションセンター
    • Related Report
      2012 Final Research Report
  • [Presentation] 無症候性キャリアにおける欠損型 HTLV-1 プロウイルス2010

    • Author(s)
      梅木一美, 竹ノ内博之, 山本成郎, 岡山昭彦, 佐々木大介, 上平憲
    • Organizer
      第57回日本臨床検査医学会学術集会
    • Place of Presentation
      東京都新宿区, 京王プラザホテル
    • Related Report
      2012 Final Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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