New diagnostic method and personalized biomarker using circulating nucleic acids in patients with digestive system cancer

• Project/Area Number: 22590533
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: ICHIKAWA Daisuke 京都府立医科大学, 医学研究科, 講師 (20347446)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: バイオマーカー / 腫瘍マーカー / microRNA / 消化器癌 / 血漿 / 循環核酸 / 術後マーカー / 個別化

Research Abstract

This study found that cell-free DNA and microRNA stably exist in the peripheral blood of patients with digestive tract cancers, and detections of tumor-specific abnormalities in the circulating nucleic acids reflect tumor dynamics of primary lesions to some de gree. This study also indicated that detection of tumor-specific amplification and microRNA in the peripheral blood could be useful as not only complementary tumor markers but also prognostic markers for digestive tract cancers.

Research Products

• [Journal Article] Hirajima S, Ichikawa D, (他 14 名,3 番目) (2013)
  • Author(s): Hirajima S, Ichikawa D
  • Journal Title: Br J Cancer Volume: 108 Issue: 9 Pages: 1822-1829
  • DOI: 10.1038/bjc.2013.148
  • Peer Reviewed
• [Journal Article] Clinical impact of circulating miR-221 in plasma of patients with pancreatic cance (2013)
  • Author(s): Kawaguchi T, Ichikawa D
  • Journal Title: Br J Cancer Volume: 108 Issue: 2 Pages: 361-369
  • DOI: 10.1038/bjc.2012.546
  • Peer Reviewed
• [Journal Article] Prognostic impact of circulating miR-21 in the plasma of patients with gastric carcinoma (2013)
  • Author(s): Komatsu S, Ichikawa D
  • Journal Title: Anticancer Res Volume: 33 Pages: 271-276
  • Peer Reviewed
• [Journal Article] Circulating microRNA in digestive tract cancers (2012)
  • Author(s): Ichikawa D, Komatsu S
  • Journal Title: Gastroenterology Volume: 142 Issue: 5 Pages: 1074-1078
  • DOI: 10.1053/j.gastro.2012.03.008
  • Peer Reviewed
• [Journal Article] Detection of gastric cancerassociated microRNAs on microRNA microarray comparing pre- and post-operative plasma (2012)
  • Author(s): Konishi H, Ichikawa D
  • Journal Title: Br J Cancer Volume: 106 Issue: 4 Pages: 740-747
  • DOI: 10.1038/bjc.2011.588
  • Peer Reviewed
• [Journal Article] Prognostic impact of circulating miR-21 and miR-375 in plasma of patients with esophageal squamous cell carcinoma (2012)
  • Author(s): Komatsu S, Ichikawa D
  • Journal Title: Expert Opin Biol Ther Volume: 12 Issue: sup1 Pages: S53-S59
  • DOI: 10.1517/14712598.2012.681373
  • Peer Reviewed
• [Journal Article] Circulating microRNAs in plasma of patients with oesophageal squamous cell carcinoma (2011)
  • Author(s): Komatsu S, Ichikawa D
  • Journal Title: Br J Cancer Volume: 105 Issue: 1 Pages: 104-111
  • DOI: 10.1038/bjc.2011.198
  • Peer Reviewed
• [Journal Article] Novel diagnostic value of circulatingmiR-18a in plasma of patients with pancreatic cancer (2011)
  • Author(s): Morimura R, Ichikawa D
  • Journal Title: Br J Cancer Volume: 105 Issue: 11 Pages: 1733-1740
  • DOI: 10.1038/bjc.2011.453
  • Peer Reviewed
• [Journal Article] Circulating microRNAs in plasma of patients with gastric cancers (2010)
  • Author(s): Tsujiura M, Ichikawa D
  • Journal Title: Br J Cancer Volume: 102 Issue: 7 Pages: 1174-1179
  • DOI: 10.1038/sj.bjc.6605608
  • Peer Reviewed
• [Journal Article] Prediction of CCND1 amplification using plasma DNA as a prognostic marker in oesophageal squamous cell carcinoma (2010)
  • Author(s): Takeshita H, Ichikawa D
  • Journal Title: Br J Cancer Volume: 102 Issue: 9 Pages: 1378-1383
  • DOI: 10.1038/sj.bjc.6605657
  • Peer Reviewed
• [Journal Article] Circulating microRNAs in plasma of patients with gastric cancers. (2010)
  • Author(s): Tsujiura M, Ichikawa D, et al.
  • Journal Title: British Journal of Cancer Volume: 102 Pages: 1174-1179
  • Peer Reviewed
• [Journal Article] Prediction of CCND1 amplification using plasma DNA as a prognostic marker in oesophageal squamous cell carcinoma. (2010)
  • Author(s): Takeshita H, Ichikawa D, et al.
  • Journal Title: British Journal of Cancer Volume: 102 Pages: 1378-1383
  • Peer Reviewed
• [Presentation] 基礎研究知見の臨床応用への翻訳‐循環遊離 microRNA のバイオマーカーとして有用性‐ (2012)
  • Author(s): 市川大輔 他
  • Organizer: 第67回日本消化器外科学会総会
  • Place of Presentation: 富山
• [Presentation] 胃癌における基礎研究知見の臨床応用‐循環遊離 microRNA のバイオマーカーとしての有用性と問題点‐ (2012)
  • Author(s): 市川大輔 他
  • Organizer: 第44回胃病態機能研究会
  • Place of Presentation: 熊本
• [Presentation] 基礎研究知見の臨床応用への翻訳‐循環遊離microRNAのバイオマーカーとして有用性‐ (2012)
  • Author(s): 市川大輔
  • Organizer: 日本消化器外科学会
  • Place of Presentation: 富山
• [Presentation] 胃癌における基礎研究知見の臨床応用 －循環遊離microRNAのバイオマーカーとしての有用性と問題点－ (2012)
  • Author(s): 市川大輔
  • Organizer: 胃病態機能研究会
  • Place of Presentation: 熊本
• [Presentation] 膵癌患者における血漿中microRNA-18a測定の意義についての検討 (2011)
  • Author(s): 市川大輔, 他
  • Organizer: 第70回日本癌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-03
• [Presentation] 膵癌患者における血漿中microRNA-18a 測定の意義についての検討 (2011)
  • Author(s): 市川大輔 他
  • Organizer: 第70回日本癌学会総会
  • Place of Presentation: 名古屋
• [Presentation] Circulating microRNAs in plasma of patients with gastric cancers (2010)
  • Author(s): 市川大輔 他
  • Organizer: 第69回日本癌学会総会
  • Place of Presentation: 大阪
• [Presentation] Circulating microRNAs in plasma of patients with gastric cancers (2010)
  • Author(s): 市川大輔, 他
  • Organizer: 第69回日本癌学会
  • Place of Presentation: 大阪