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Membrane proteomic analysis of cancer tissue for identification of biomarkers for early diagnosis

Research Project

Project/Area Number 22590545
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research Institution独立行政法人医薬基盤研究所

Principal Investigator

KUME Hideaki  独立行政法人医薬基盤研究所, 創薬基盤研究部, 研究員 (50322714)

Co-Investigator(Kenkyū-buntansha) TOMONAGA Takeshi  独立行政法人医薬基盤研究所, 創薬基盤研究部, プロジェクトリーダー (80227644)
MATSUBARA Hisahiro  千葉大学, 医学(系)・研究科(研究院), 教授 (20282486)
ISHIHAMA Yasushi  京都大学, 薬学研究科(研究院), 教授 (30439244)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords腫瘍検査学 / 癌 / プロテオーム
Research Abstract

We performed quantitative proteomic analysis of membrane fractions from colorectal cancer tissue for biomarker discovery and extensively validated biomarker candidate proteins. A total of 5287 of 5566 identified proteins were annotated by GO cellular components analysis and 3087 were predicted to be membrane proteins. Also, 1567 proteins were predicted to have a transmembrane domain by TMHMM algorithm. Over 100 of proteins were differentially expressed between polyps and cancer or between cancer with and without metastasis. Among these biomarker candidates, 105 proteins were further quantitated by selected reaction monitoring (SRM) using synthetic stable isotope-labeled peptides as internal control and 78 were verified. The expression of two candidate proteins, protein-X and -Y, were also confirmed by Western blotting and immunostaining. Furthermore, analysis of multi-cancer tissue microarray consisting of 1150 cores from 14 cancer tissues showed that these proteins were highly expressed not only in colorectal cancer but also in several cancer tissues, suggesting that these proteins have the potential to be biomarker for stomach, breast and prostate cancer as well as colon cancer.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (22 results)

All 2012 2011 2010 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (16 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] In-depth Membrane Proteomic Study of Breast Cancer Tissues for the Generation of a Chromosome-based Protein List2012

    • Author(s)
      Muraoka S, Kume H, Adachi J, Shiromizu T, Watanabe S, Masuda T, Ishihama Y, Tomonaga T
    • Journal Title

      J Proteome Res

      Volume: 12(1) Issue: 1 Pages: 208-13

    • DOI

      10.1021/pr300824m

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A strategy for large-scale phosphoproteomics and SRM-based validation of human breast cancer tissue samples.2012

    • Author(s)
      Narumi R, Murakami T, Kuga T, Adachi J, Shiromizu T, Muraoka S, Kume H, Kodera Y, Matsumoto M, Nakayama K, Miyamoto Y, Ishitobi M, Inaji H, Kato K, Tomonaga T.
    • Journal Title

      J Proteome Res.

      Volume: 11 Issue: 11 Pages: 5311-5322

    • DOI

      10.1021/pr3005474

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Strategy for SRM-based verification of biomarker candidates discovered by iTRAQ method in limited breast cancer tissue samples.2012

    • Author(s)
      Muraoka S, Kume H, Watanabe S, Adachi J, Kuwano M, Sato M, Kawasaki N, Kodera Y, Ishitobi M, Inaji H, Miyamoto Y, Kato K, Tomonaga T.
    • Journal Title

      J Proteome Res.

      Volume: 11 Issue: 8 Pages: 4201-4210

    • DOI

      10.1021/pr300322q

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Presentation] 大規模プロテオーム解析による大腸癌バイオマーカーの探索とその検証2012

    • Author(s)
      久米秀明、村岡賢、小寺義男、松下一之、松原久裕、朝長毅
    • Organizer
      第71回日本癌学会
    • Place of Presentation
      札幌
    • Year and Date
      2012-09-21
    • Related Report
      2012 Final Research Report
  • [Presentation] 大腸癌組織膜タンパク質の大規模プロテオーム解析によるバイオマーカー探索とその検証2012

    • Author(s)
      久米秀明、渡邊史生、村岡賢、石濱泰、小寺義男、松下一之、松原久裕、朝長毅
    • Organizer
      第10回日本プロテオーム学会
    • Place of Presentation
      東京
    • Year and Date
      2012-07-26
    • Related Report
      2012 Final Research Report
  • [Presentation] 大腸癌組織膜タンパク質の大規模プロテオーム解析によるバイオマーカー探索とその検証2012

    • Author(s)
      久米秀明,渡邊史生,村岡賢,石濱泰,小寺義男,松下一之,松原久裕,朝長毅
    • Organizer
      日本プロテオーム学会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] 大規模プロテオーム解析による大腸癌バイオマーカーの探索とその検証2012

    • Author(s)
      久米秀明,村岡賢,小寺義男,松下一之,松原久裕,朝長毅
    • Organizer
      日本癌学会
    • Place of Presentation
      札幌
    • Related Report
      2012 Annual Research Report
  • [Presentation] 大腸癌組織膜タンパク質の大規模プロテオーム解析によるバイオマーカー探索とSRM/MRM法を用いた定量法の確立および診断への応用2011

    • Author(s)
      久米秀明, 鳴海良平, 渡邉史生,石濱泰, 松原久裕, 小寺義男, 福岡順也, 朝長毅
    • Organizer
      第34回分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-14
    • Related Report
      2012 Final Research Report
  • [Presentation] 大腸癌組織膜タンパク質の大規模プロテオーム解析によるバイオマーカー探索とSRM/MRM法を用いた定量法の確立および診断への応用2011

    • Author(s)
      久米秀明
    • Organizer
      日本分子生物学会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 膜タンパク質の大規模プロテオーム解析による大腸癌バイオマーカーの探索とSRM法を用いた検証2011

    • Author(s)
      久米秀明, 松原久裕, 小寺義男, 朝長毅
    • Organizer
      第70回日本癌学会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-10-04
    • Related Report
      2012 Final Research Report
  • [Presentation] 膜タンパク質の大規模プロテオーム解析による大腸癌バイオマーカーの探索とSRM法を用いた検証2011

    • Author(s)
      久米秀明
    • Organizer
      日本癌学会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-10-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] 大腸癌バイオマーカーとなる膜タンパク質の大規模プロテオーム解析とSRMによる検証2011

    • Author(s)
      久米秀明, 鳴海良平, 渡邉史生,石濱泰, 松原久裕, 小寺義男, 朝長毅
    • Organizer
      第9回日本プロテオーム学会
    • Place of Presentation
      新潟
    • Year and Date
      2011-07-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 大腸癌バイオマーカーとなる膜タンパク質の大規模プロテオーム解析とSRMによる検証2011

    • Author(s)
      久米秀明
    • Organizer
      日本プロテオーム学会
    • Place of Presentation
      新潟
    • Year and Date
      2011-07-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 大腸癌の新たなバイオマーカーとなる膜タンパク質の探2010

    • Author(s)
      久米秀明, 鳴海良平, 石濱泰, 松原久裕, 松下一之, 野村文夫,朝長毅
    • Organizer
      第33回分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-04
    • Related Report
      2012 Final Research Report
  • [Presentation] 大腸癌の新たなバイオマーカーとなる膜タンパク質の探索2010

    • Author(s)
      久米秀明
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] 大腸癌組織膜タンパク質のプロテオーム解析2010

    • Author(s)
      久米秀明, 松原久裕, 松下一之,野村文夫,朝長毅
    • Organizer
      第69回日本癌学会
    • Place of Presentation
      大阪
    • Year and Date
      2010-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] 大腸癌組織膜タンパク質のプロテオーム解析2010

    • Author(s)
      久米秀明
    • Organizer
      日本癌学会
    • Place of Presentation
      大阪
    • Year and Date
      2010-09-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] 大腸癌組織膜タンパク質のプロテオーム解析2010

    • Author(s)
      久米秀明、鳴海良平、石濱泰、松原久裕、松下一之、野村文夫、朝長毅
    • Organizer
      第8回日本プロテオーム学会
    • Place of Presentation
      舞浜
    • Year and Date
      2010-07-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 大腸癌組織膜タンパク質のプロテオーム解析2010

    • Author(s)
      久米秀明
    • Organizer
      日本プロテオーム学会
    • Place of Presentation
      舞浜
    • Year and Date
      2010-07-27
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.nibio.go.jp/proteome/index.html

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 大腸がんの判定方法2012

    • Inventor(s)
      朝長毅、久米秀明
    • Industrial Property Rights Holder
      独立行政法人医薬基盤研究所
    • Filing Date
      2012-12-17
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 大腸がんの判定方法2012

    • Inventor(s)
      朝長毅、久米秀明
    • Industrial Property Rights Holder
      独立行政法人医薬基盤研究所
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-12-27
    • Related Report
      2012 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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