| Project/Area Number |
22590570
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | National Institute of Public Health |
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Principal Investigator |
USHIYAMA Akira 国立保健医療科学院, 生活環境研究部, 上席主任研究官 (60291118)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 衛生 / 環境生理 / 微小循環 / 紫外線 / バイオイメージング / 抗酸化物質 / 紫外線影響 / 動物 / 抗酸化作用 |
|---|
| Research Abstract |
OBJECTIVE: We previously demonstrated that ultraviolet B (UVB: wave length 280 . 315 nm) irradiation to mouse skin induced acute microcirculatory effects, i.e., vascular dilation, increase in vascular permeability and leukocyte-endothelial interaction, which was caused by reactive oxygen due to UVB. The objective of the present study was to evaluate effects of anti-oxidants such as vitamin E (VE) and green tea catechins (GTC) on leukocyte-endothelial interactions by UVB irradiation (UVBIR) using the mouse-dorsal skin chamber (DSC) as an experimental model.
METHODS: DSCs were installed to female hairless HR-1 mice by surgical procedure at least 72 hours prior to the experiment. We examined 4 experimental conditions; UVBIR only, anti P-selectin antibody treatment+UVBIR, VE administration+UVBIR and GTC administration+UVBIR. To visualize the leukocytes behaviors in vivo, a bolus of rhodamine 6G solution was injected into tail vein prior observation. Before and after UVBIR (240 mJ/cm2) to th
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e skin within the chamber, fluorescent images at the same microvasculature were recorded by using a fluorescence microscopy under conscious conditions. Off-line analysis of the leukocyte-endothelial interaction was done by counting numbers adhering and rolling leukocytes within venules for 30 sec per unit area. VE was administrated twice a day (25 μl/each) for 3 days and 1 hour before the UVBIR via feeding tube. Dissolved GTC in water (10mg/each) was administrated 1 hour before the UVBIR via feeding tube. RESULTS: The mean value of rolling leukocytes number was significantly higher in the venules at 6 hours after UVBIR than that in pre-UVBIR. This increase was suppressed when anti-mouse P-selectin antibody was injected into tail vein prior to UVBIR. When VE or GTC was administrated prior to UVBIR, increase of rolling leukocytes number was also suppressed.
CONCLUSIONS: Our findings suggest that the administrations of anti-oxidants show the preventive effect on UVB induced leukocyte-endothelial interaction which is mainly mediated by P-selectin. Less
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