Experimental verification for utilization of expression profiles of human metallothionein isoforms as a biomarker for X-ray irradiation

Research Project

Project/Area Number	22590571
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Hygiene
Research Institution	National Institute of Occupational Safety and Health, Japan
Principal Investigator	MIURA Nobuhiko 独立行政法人労働安全衛生総合研究所, 健康障害予防研究グループ, 主任研究員 (20229644)
Co-Investigator(Kenkyū-buntansha)	木村真三独立行政法人労働安全衛生総合研究所, 人間工学リスク管理(II)研究グループ, 研究員 (50321849)
Project Period (FY)	2010 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	放射線 / メタロチオネイン / バイオマーカー
Research Abstract	To investigate the expression profile(s) of human metallothionein isoform genes after X-ray irradiation, human cultured cells irradiated by X-ray (1 Gy to 16 Gy) were collected at 24 h after the irradiation. The expression levels of each gene were estimated by real-time PCR. Most of isoform genes including the main isoform genes such as MT-2A and MT-1X were inhibited of their expression, while induced of them for MT-1H and MT-4. This expression pattern was different from the induction pattern by heavy metals, suggesting be a biomarker for X-ray irradiation.