| Project/Area Number |
22590573
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Hygiene
|
|---|
| Research Institution | Osaka Prefectural Institute of Public Health |
|---|
Principal Investigator |
MORI Haruyo 大阪府立公衆衛生研究所, 感染症部, 主任研究員 (20250300)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
小島 洋子 大阪府立公衆衛生研究所, 感染症部, 主任研究員 (70291218)
川畑 拓也 大阪府立公衆衛生研究所, 感染症部, 主任研究員 (80270768)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | HIV-1 / superinfection / 重複感染 / 特異プライマー / HIV |
|---|
| Research Abstract |
In order to detect HIV-1 superinfection (or dual-infection), we constructed three sets of primers that were designed to specifically amplify HIV-1 strains forming large clusters in Osaka. Proviral DNA samples obtained from 63 HIV-1-infected patients were analysed by nested PCR using the cluster-specific primers, and two cases were found to be dually infected with two distinct HIV-1 subtype B.
|
