| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
Non-coding and small single-stranded RNA molecules, microRNAs (miRNAs), have been found to regulate a variety of physiological functions. We focused on the behavior of miRNAs at postmortem as a biomarker to quantify the physical stress. In this preliminary study on rat, we determined a number of miRNAs that exist after death and found their possibility as a useful tool to as a biomarker to determine the cause of death. Unfortunately, this study revealed that it was prohibitively difficult to analyze the total RNA including miRNAs using the LC/MS/MS directly. We performed comprehensive microarray analysis of rat miRNAs after their death.
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