Connexin-43 based hemi-channel contributes to the propagation of μ-calpain mediated neuronal death in rat brain cortex

• Project/Area Number: 22590628
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Legal medicine
• Research Institution: National Defense Medical College (2011-2012); The University of Tokyo (2010)
• Principal Investigator: HARADA Kazuki 防衛医科大学校, 医学教育部医学科専門課程, 准教授 (00253146)
• Co-Investigator(Kenkyū-buntansha): OSHIDA Ken-ichi 東京大学, 医学(系)研究科(研究院), 教授 (40166947)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2010: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
• Keywords: 神経細胞死 / 皮質層状壊死 / ヘミチャンネル / アストログリア / カルパイン / コネクシン43 / 医療・福祉 / 脳・神経 / 病理学 / コネクシン / 脳損傷 / ネクローシス / ギャップ結合 / 神経細胞

Research Abstract

We examined the contribution of GJ or HC on astrocytes or neurons to TBI spreading in a stab wound model in the rat cortex, and we demonstrated the importance of Cx43-based HC in the spread of μ-calpain-dependent neuronal death.

Research Products

• [Journal Article] Restraint Stress Induces Connexin-43 Translocation via a-Adrenoceptors in Rat Heart. (2010)
  • Author(s): Unuma K, Shintani-Ishida K, Yahagi N, Tsushima K, Shimosawa T, Ueyama T, Yoshida K
  • Journal Title: Circulation Journal Volume: 74(6) Pages: 1087-1095
  • Peer Reviewed
• [Journal Article] Localization and mechanism of nephrocalcinosis in pseudo-Bartter's syndrome. (2010)
  • Author(s): Tojo A, Unuma K, Harada K, Yoshida K, Fujita T
  • Journal Title: Kidney International Volume: 77(9) Pages: 831-831
  • Peer Reviewed
• [Journal Article] Brief exposure to carbon monoxide preconditions cardiomyogenic cells against apoptosis in ischemia-reperfusion. (2010)
  • Author(s): Kondo-Nakamura M, Shintani-Ishida K, Uemura K, Yoshida K.
  • Journal Title: Biochemistry Biophysics Research Communication Volume: 393(3) Pages: 449-454
  • Peer Reviewed
• [Journal Article] Yoshida K.Connexin-43 redistribution and gap junction activation during forced restraint protects against sudden arrhythmic death in rats. (2010)
  • Author(s): Unuma K, Shintani-Ishida K, Tsushima K, Shimosawa T, Ueyama T, Kuwahara M
  • Journal Title: Circulation Journal Volume: 74(6) Pages: 1087-1095
  • Peer Reviewed
• [Journal Article] Legal Medicine (2010)
  • Author(s): Unuma K, Harada K, Nakajima M, Ito T, Okutsu K, Yoshida K
  • Journal Title: Unexpected death of a patient with rheumatoid arthritis complicated by acervical deformity Volume: 12(5) Pages: 242-245
  • Peer Reviewed
• [Presentation] Gap Junction blocker reduces spreading of traumatic brain injury in rat (2011)
  • Author(s): 石井康博、新谷香、原田一樹、吉田謙一
  • Organizer: 第95次日本法医学会学術全国集会
  • Place of Presentation: 福島県福島市
  • Year and Date: 2011-06-17
• [Presentation] Gap Junction blocker reduces spreading of traumatic brain injury in rat (2011)
  • Author(s): 石井康博, 新谷香, 原田一樹, 吉田謙一
  • Organizer: 第95次日本法医学会学術全国集会
  • Place of Presentation: 福島県福島市コラッセ福島
  • Year and Date: 2011-06-17
• [Book] 事例に学ぶ法医学・医事法 (2010)
  • Author(s): 吉田謙一
  • Total Pages: 411
  • Publisher: 有斐閣