Proteomic screening of possible marker proteins for drug-induced cellular injuries
| Project/Area Number |
22590629
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
AKI Toshihiko 東京医科歯科大学, 大学院・医歯学総合研究科, 講師 (60304474)
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| Co-Investigator(Kenkyū-buntansha) |
UEMURA Koichi 東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (30244586)
船越 丈司 東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (40444715)
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| Co-Investigator(Renkei-kenkyūsha) |
FUNAKOSHI Takeshi 東京医科歯科大学, 大学院・医歯学総合研究科, 助教 (40444715)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 法医学 / プロテオーム / プロテオミクス / 覚せい剤 / マクロピノサイトース / オートファジー / 細胞障害 / ミトコンドリア / コカイン / アルコール |
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| Research Abstract |
Using human nueroblastoma cells, rat cardiomyocyte-derived cells, and rat liver as well as heart, molecular mechanisms of cytotoxicity of several toxicants including metamphetamine, cocaine, and LPS were examined. Major-responsible proteins were determined by the method of proteomics. Involvement of macropinocytosis, myosin gene expression, and active extrusion of mitochondrial proteins were suggested during methamphetamine, cocaine, and LPS exposure, respectively. These results have proved usefulness of the method of proteomics in the field of forensic toxicology.
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Report
(4 results)
Research Products
(34 results)
