Detection of body fluid type and infection disease through DNA chipanalysis

• Project/Area Number: 22590641
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: IKEGAYA Hiroshi 京都府立医科大学, 医学研究科, 教授 (30292874)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 法医鑑定学 / DNAチップ / 感染症 / 体液識別法 / 血液型判定法 / 血液型

Research Abstract

In addition to the blood type, HBV and HCV were detected through DNA chip analysis during a single process. These viruses were detectable and infectious in postmortem cases. Additionally, we also succeeded in detecting body fluid specific mRNA.

Research Products

• [Journal Article] Use of the Genome Profiling Method for the Identification of Saliva and Sweat Samples (2013)
  • Author(s): Hirata R, Takasaka T, Miyamori D, Ahmed S, Sakudara K, Nishigaki K, Ikegaya H
  • Journal Title: the Identification of Saliva and Sweat Samples Volume: 18 Pages: 79-83
  • NAID: 130004485383
• [Journal Article] Forensic validation of the modified ABO genotyping method using a DNA chip. (2013)
  • Author(s): Watanabe K
  • Journal Title: Leg Med Volume: 未定 Issue: 4 Pages: 222-225
  • DOI: 10.1016/j.legalmed.2013.01.004
  • Peer Reviewed
• [Journal Article] Use of the Genome Profiling Method for the Identification of Saliva and Sweat Samples (2013)
  • Author(s): Hirata R
  • Journal Title: Jp J Forensic Sci Tech Volume: 18 Pages: 79-83
  • Peer Reviewed
• [Journal Article] etection of hepatitis C virus and antibodies in postmortem blood and bloodstains (2011)
  • Author(s): Takasaka T, Itoh Y, Kaneko H, Ikegaya H
  • Journal Title: J Clin Microbiol Volume: 49 Pages: 1122-1123
• [Journal Article] Takasaka T, Sakurada K, Akutsu T, Nishigaki K, Ikegaya H (2011)
  • Author(s): Takasaka T, Sakurada K, Akutsu T, Nishigaki K, Ikegaya H
  • Journal Title: Leg Med Volume: 13 Pages: 265-267
• [Journal Article] A novel method for ABO genotyping using a DNA chip (2011)
  • Author(s): Watanabe K, Ikegaya H, Hirayama K, Motani H, Iwase H, Kaneko H, Fukushima H, Akutsu T, Sakurada K
  • Journal Title: J Forensic Sci Volume: 56S1 Pages: 183-187
• [Journal Article] Detection of hepatitis C virus and antibodies in postmortem blood and bloodstains (2011)
  • Author(s): Takasaka T, Itoh Y, Kaneko H, Ikegaya H
  • Journal Title: J Clin Microbiol Volume: 49 Issue: 3 Pages: 1122-1123
  • DOI: 10.1128/jcm.01887-10
  • Peer Reviewed
• [Journal Article] Trials of the detection of semen and vaginal fluid RNA using the genome profiling method (2011)
  • Author(s): Takasaka T, Sakurada K, Akutsu T, Nishigaki K, Ikegaya H
  • Journal Title: Leg Med Volume: 13(5) Issue: 5 Pages: 265-267
  • DOI: 10.1016/j.legalmed.2011.05.001
  • Peer Reviewed
• [Journal Article] Detection of hepatitis C virus and antibodies in postmortem blood and bloodstains. (2011)
  • Author(s): Takasaka T, Itoh Y, Kaneko H, Ikegaya H
  • Journal Title: J Clin Microbiol. Volume: 49 Pages: 1122-1123
  • Peer Reviewed
• [Journal Article] Forensic validation of the modified ABO genotyping method using a DNA chip
  • Author(s): Watanabe K, Hosoya M, Hirayama K, Saitoh H, Iwase H, Ikegaya H, Akutsu T, Sekiguchi K, Sakurada K
  • Journal Title: Leg Med
• [Presentation] DNAチップを用いたABO遺伝子型判定法の検討 (2012)
  • Author(s): 渡邊 賢
  • Organizer: 日本法科学技術学会
  • Place of Presentation: 東京
• [Presentation] DNAチップを用いた ABO 遺伝子型判定法の検討 (2011)
  • Author(s): 渡邊 賢,細谷 真悠子,平山 幸一,斉藤 久子,岩瀬 博太郎,池谷 博,阿久津 智子,関口 和正,櫻田 宏一
  • Organizer: 日本法科学技術学会
  • Place of Presentation: 東京
  • Year and Date: 2011-11-16
• [Presentation] 血痕からの肝炎ウイルス感染の危険性 (2011)
  • Author(s): 原隼平,高坂友和,石川 昂,小松亜由美,吉本寛司,池谷 博
  • Organizer: 第94 次日本法医学会学術全国集会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-24
• [Presentation] A new method of identifying humans using genome profiling (2011)
  • Author(s): 諏訪渚、高坂友和、石川昴、吉本寛司、池谷博
  • Organizer: 第95次日本法医学会総会
  • Place of Presentation: 福島
  • Year and Date: 2011-06-17
• [Presentation] 血痕からの肝炎ウイルス感染の危険性 (2010)
  • Author(s): 原隼平, 高坂友和, 石川昂, 小松亜由美, 吉本寛司, 池谷博
  • Organizer: 第94次日本法医学会学術全国集会
  • Place of Presentation: 東京
  • Year and Date: 2010-06-24