construction of the rapid screening method for the poisonous substances which can be judged during autopsy

• Project/Area Number: 22590645
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Legal medicine
• Research Institution: National Research Institute of Police Science
• Principal Investigator: INOUE Hiroyuki 科学警察研究所, 法科学研修所, 主任教授 (40159992)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 社会医学 / 分析科学 / 法中毒学 / 質量分析 / 薬毒物 / スクリーニング / 血液 / 違法薬物

Research Abstract

In the present study, construction of the rapid screening method for the poisonous substances is described. Target substances were chosen from the list of drugs selected as the most important compounds for toxicological analysis in Japan. Human plasma extracts with solid-phase extraction cartridges were determined by an ion-trap mass spectrometer. Collision-induced dissociation of protonated molecules gave characteristic product ion spectra and provided identification of the analytes. Time required for one sample was 5 min or less. The method seems to be useful for rapid screening of the poisonous substances in the practice of forensic science

Research Products

• [Journal Article] Differentiation of regioisomeric fluoroamphetamine analogs by gas chromatography.mass spectrometry and liquid chromatography.tandem mass spectrometry (2013)
  • Author(s): Nakazono, Y., Tsujikawa, K., Kuwayama, K., Kanamori, T., Iwata, Y.T., Miyamoto, K., Kasuya, F., Inoue, H
  • Journal Title: Forensic Toxicology Volume: (in press) Issue: 2 Pages: 241-250
  • DOI: 10.1007/s11419-013-0184-7
  • Peer Reviewed
• [Presentation] Drug screening in human plasma using solid phase extraction and direct mass spectrometry (2013)
  • Author(s): Inoue, H., Nakazono, Y., Hashimoto, H., Kuwayama, K., Tsujikawa, K., Kanamori, T.,Iwata, Y.T
  • Organizer: 米国分析機器展示会(PITTCON)
  • Place of Presentation: 米国,フィラデルフィア
  • Year and Date: 2013-03-18
• [Presentation] Direct analysis of amphetamines in urine by headspace atmospheric pressure chemical ionization/ion trap mass spectrometry (2012)
  • Author(s): Inoue, H., Nakazono, Y., Tsujikawa, K., Hashimoto, M., Umeda, M., Yamada, M., Miyamoto, K., Kasuya, F
  • Organizer: Direct analysis of amphetamines in urine by headspace atmospheric pressure chemical ionization/ion trap mass spectrometry
  • Place of Presentation: 豪州,ホバート
  • Year and Date: 2012-09-24
• [Presentation] Analysis of amphetamines and ephedrines in urine using solid phase extraction and direct mass spectrometry (2012)
  • Author(s): Inoue, H., Hashimoto, H., Nakazono, Y., Miyaguchi, H., Kuwayama, K., Tsujikawa, K., Kanamori, T., Iwata, Y.T., Fukushima, H
  • Organizer: 米国法中毒学会-国際法中毒学会 合同会議
  • Place of Presentation: 米国,サンフランシスコ
  • Year and Date: 2012-08-06
• [Presentation] Differentiation of regioisomeric methoxyamphetamines and fluoroamphetamines by GC/MS and LC/MS (2012)
  • Author(s): Nakazono, Y., Tsujikawa, K., Kanamori, T., Kuwayama, K., Iwata, Y. T., Miyaguchi, H., Yamada, M., Miyamoto, K., Kasuya, F., Inoue, H
  • Organizer: 国際法中毒学会(TIAFT)
  • Place of Presentation: 浜松
  • Year and Date: 2012-06-07
• [Presentation] Forensic Toxicology . Detection and Profiling of Drugs and PoisonPITTCON (2011)
  • Author(s): Inoue, H
  • Organizer: 国際犯罪学会第16回世界大会
  • Place of Presentation: 神戸
  • Year and Date: 2011-09-28
• [Presentation] Analysis of amphetamines and ephedrines in urine using solid phase extraction and direct mass spectrometry (2011)
  • Author(s): Inoue, H., et al
  • Organizer: 2011 SOFT-TIAFT(米国法中毒学会-国際法中毒学会合同会議)
  • Place of Presentation: サンフランシスコマリオットマルキスホテル
  • Year and Date: 2011-09-28
• [Presentation] Forensic toxicology-Detection and profiling of drugs and poison- (2011)
  • Author(s): Inoue, H
  • Organizer: 国際犯罪学会第16回世界大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2011-08-06
• [Presentation] Direct analysis of illicit drugs by thermal desorption counter-flow introduction atmospheric pressure chemical ionization mass spectrometry (2011)
  • Author(s): Inoue, H., Hashimoto, H., Iwata, Y.T., Tsujikawa, K., Hashimoto, M., Yokokura, T., Yamada, M
  • Organizer: IUPAC2011国際分析科学会議(ICAS2011)
  • Place of Presentation: 京都
  • Year and Date: 2011-05-25
• [Presentation] Direct analysis of illicit drugs by thermal desorption counter-flow introduction atmospheric pressure chemical ionization mass spectrometry (2011)
  • Author(s): Inoue, H., et al
  • Organizer: IUPAC2011国際分析科学会議(ICAS2011)
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2011-05-25
• [Presentation] Rapid urinalysis of amphetamines by thermal desorption counter flow introduction atmospheric pressure chemical ionization mass spectrometry (2010)
  • Author(s): Inoue, H., Hashimoto, H., Watanabe, S., Iwata, Y.T., Kanamori, T., Miyaguchi, H., Tsujikawa, K., Kuwayama, K., Mikuma, T., Yokokura, T., Yamada, M
  • Organizer: PACIFICHEM2010
  • Place of Presentation: 米国,ホノルル
  • Year and Date: 2010-12-18
• [Presentation] Rapid urinalysis of amphetamines by thermal desorption counter flow introduction atmospheric pressure chemical ionization mass spectrometry. (2010)
  • Author(s): Inoue, H, et al.
  • Organizer: 2010環太平洋国際化学会議(Pacifichem2010)
  • Place of Presentation: コンベンションセンター(米国、ホノルル)
  • Year and Date: 2010-12-18
• [Presentation] Differentiation of regioisomeric methoxyamphetamines and fluoroamphetamines by GC/MS and LC/MS
  • Author(s): Yukiko Nakazono
  • Organizer: 国際法中毒学会（TIAFT2012）
  • Place of Presentation: 浜松
• [Presentation] Drug screening in human plasma using solid phase extraction and direct mass spectrometry
  • Author(s): Hiroyuki Inoue
  • Organizer: 米国分析機器展示会（Pittcon2013）
  • Place of Presentation: 米国フィラデルフィア