Development of mucosal vaccine based on mucosal immune system enhancing activity of Kampo medicines.

• Project/Area Number: 22590672
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General internal medicine (including Psychosomatic medicine)
• Research Institution: Sojo University
• Principal Investigator: MIYATA Takeshi 崇城大学, 薬学部, 客員研究員 (90040310)
• Co-Investigator(Kenkyū-buntansha): ISOHAMA Yoichiro 熊本大学, 大学院・生命科学研究部, 准教授 (10240920) ; YOKOMIZO Kazumi 崇城大学, 薬学部, 教授 (80240919)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 漢方薬 / インフルエンザ / 東洋医学 / 粘膜ワクチン / アジュバント

Research Abstract

Intranasal administration of hemagglutinin vaccine with an adjuvant is effective for protection against influenza virus infection. In this study, we found that Kampo medicines, which enhance mucosal immune system, are used for an oral adjuvant. Maoto increased IgA, IgG antibody in bronchoalveolar lavage fluid and serum. Maoto also showed production of virus-specific antibody after intranasal vaccination.

Research Products

• [Journal Article] Endogenous acetylcholine rescues NMDA-induced long-lasting hippocampal cell damage via stimulation of muscarinic M(1) receptors elucidation using organic hippocampal slice cultures. (2013)
  • Author(s): Inada C, Thi Le X, Tsuneyama K, Fujiwara H, Miyata T, Matsumoto K.
  • Journal Title: Eur J Pharmacol Volume: 699 Pages: 150-159
  • Peer Reviewed
• [Journal Article] Chotosan ameliorates cognitive and emotional deficits in an animal model of type 2 diabetes: possible involvement of cholinergic andVEGF/PDGF mechanisms (2012)
  • Author(s): Zhao Q, Niu Y, Matsumoto K, Tsuneyama K, Tanaka K, Miyata T, Yokozawa T.
  • Journal Title: BMC Complement Altern Med Volume: 12 Pages: 188-188
  • Peer Reviewed
• [Journal Article] Caffeic acid phenethyl ester protects nigral dopaminergic neurons via dual mechanisms involving haem oxygenase-1 and brain-derived neurotrophic factor. (2012)
  • Author(s): Kurauchi Y, Hisatsune A, Isohama Y, Mishima S, Katsuki H.
  • Journal Title: Br J Pharmacol. Volume: 166 Pages: 1151-1168
  • Peer Reviewed
• [Journal Article] Molecular symmetry and biological activitiesof new symmetrical tris(2-aminoethyl)amine derivatives. (2012)
  • Author(s): Mibu N, Yokomizo K, Uchida W, Takemura S, Zhou J, Aki H, Miyata T, Sumoto K
  • Journal Title: Chem Pharm Bull Volume: 60 Pages: 408-414
  • Peer Reviewed
• [Journal Article] New spirostanol glycosides from Solanum nigrum and S.jasminoides. (2012)
  • Author(s): Ohno M, Murakami K, El-Aasr M, Zhou JR, Yokomizo K, Ono M, Nohara T.
  • Journal Title: J Nat Med Volume: 66 Pages: 658-663
  • Peer Reviewed
• [Journal Article] Molecular symmetry and biological activities of new symmetrical tris(2-aminoethyl)amine derivatives (2012)
  • Author(s): Mibu N
  • Journal Title: Chem Pharm Bull Volume: 60 Pages: 408-414
  • Peer Reviewed
• [Journal Article] HMGB1inhibitor glycyrrhizin attenuates intracerebral hemorrhage-induced injury in rats. (2011)
  • Author(s): Ohnishi M, Katsuki H, Fukutomi C, Takahashi M, Motomura M, Fukunaga M, Matsuoka Y, Isohama Y, Izumi Y, Kume T, Inoue A, Akaike A.
  • Journal Title: Neuropharmacology. Volume: 61 Pages: 975-980
  • NAID: 120006374513
  • Peer Reviewed
• [Journal Article] Anti-MUC1 antibody inhibits EGF receptor signaling in cancer cells. (2011)
  • Author(s): Hisatsune A, Nakayama H, Kawasaki M, Horie I, Miyata T, Isohama Y, Kim KC, Katsuki H.
  • Journal Title: Biochem Biophys Res Commun. Volume: 405 Pages: 377-381
  • Peer Reviewed
• [Journal Article] Diabetes-induced central cholinergic neuronal loss and cognitivedeficit are attenuated by tacrine and aChinese herbal prescription,kangen-karyu: elucidation in type 2 diabetes db/db mice. (2011)
  • Author(s): Zhao Q, Matsumoto K, Tsuneyama K, Tanaka K, Li F, Shibahara N, Miyata T, Yokozawa T.
  • Journal Title: J Pharmacol Sci Volume: 117 Pages: 230-242
  • Peer Reviewed
• [Journal Article] Synthesis and antiviral activities of some N-acyl-2,6-diaminopyridines and related linker mode identical twin drugs. (2011)
  • Author(s): Mibu N, Yokomizo K, Saisho M, Oishi M, Aki H, Miyata T, Sumoto K.
  • Journal Title: Heterocycles Volume: 83 Pages: 385-393
  • Peer Reviewed
• [Journal Article] Ameliorative effects of yokukansan on learning and memory deficits in olfactory bulbectomized mice. (2011)
  • Author(s): Yamada M, Hayashida M, Zhao Q, Shibahara N, Tanaka K, Miyata T, Matsumoto K.
  • Journal Title: J Ethnopharmacol Volume: 135 Pages: 737-746
  • Peer Reviewed
• [Journal Article] Chotosan (Diaoteng San)-induced improvement of cognitive deficits in senescence-accelerated mouse (SAMP8) involves the amelioration of angiogenic/neurotrophic factors and neuroplasticity systems in the brain. (2011)
  • Author(s): Zhao Q, Yokozawa T, Tsuneyama K, Tanaka K, Miyata T, Shibahara N, Matsumoto K.
  • Journal Title: Chin Med Volume: 6 Pages: 33-33
  • Peer Reviewed
• [Journal Article] Identification and characterization of component organic and glycosidic acids of crude resin glycoside fraction from Calystegia soldanella. (2011)
  • Author(s): Takigawa A, Setoguchi H, Okawa M, Kinjo J, Miyashita H, Yokomizo K, Yoshimitsu H, Nohara T, Ono M.
  • Journal Title: Chem Pharm Bull Volume: 59 Pages: 1163-1168
  • NAID: 130001852319
  • Peer Reviewed
• [Journal Article] Novel approach to curatives of Mibyou (2011)
  • Author(s): Miyata T.
  • Journal Title: Yakugaku Zasshi Volume: 131 Pages: 1289-1298
  • Peer Reviewed
• [Journal Article] Synthesis and antiviral activities of some N-acyl-2,6-diaminopyridines and related linker mode identical twin drugs (2011)
  • Author(s): Mibu N
  • Journal Title: Heterocycles Volume: VOL.83 Issue: 2 Pages: 385-393
  • DOI: 10.3987/com-10-12105
  • Peer Reviewed
• [Journal Article] Anti-MUC1 antibody inhibits EGF receptor signaling in cancer cells (2011)
  • Author(s): Hisatsune A
  • Journal Title: Biochem Biophys Res Commun Volume: VOL.405 Issue: 3 Pages: 377-381
  • DOI: 10.1016/j.bbrc.2011.01.029
  • Peer Reviewed
• [Journal Article] Identification and Characterization of Component Organic and Glycosidic Acids of Crude Resin Glycoside Fraction from Calystegia soldanella (2011)
  • Author(s): Takigawa A
  • Journal Title: Chemical and Pharmaceutical Bulletin Volume: 59 Issue: 9 Pages: 1163-1168
  • DOI: 10.1248/cpb.59.1163
  • NAID: 130001852319
  • ISSN: 0009-2363, 1347-5223
  • Peer Reviewed
• [Journal Article] Glycyrrhizin attenuates mucusproduction by inhibition of MUC5AC mRNA expression in vivo and in vitro. (2010)
  • Author(s): Nishimoto Y, Hisatsune A, Katsuki H, Miyata T, Yokomizo K, Isohama Y.
  • Journal Title: J Pharma Sci Volume: 113 Pages: 76-83
  • Peer Reviewed
• [Journal Article] Low direct cytotoxicity of loxoprof en on gastric mucosal cells. (2010)
  • Author(s): Yamakawa N, Suemasu S, Kimoto A, Arai Y, Ishihara T, Yokomizo K, Okamoto Y, Otsuka M, Tanaka K, Mizushima T.
  • Journal Title: Biol Pharm Bull Volume: 33 Pages: 398-403
  • NAID: 130000248088
  • Peer Reviewed
• [Journal Article] HSP70 inducers fromChinese herbs and their effect on melanin production. (2010)
  • Author(s): Yamashita Y, Hoshino T, Matsuda M, Kobayashi C, Tominaga A, Nakamura Y, Nakashima K, Yokomizo K, Ikeda T, Mineda K, Maji D, Niwano Y, Mizushima T.
  • Journal Title: Exp Dermatol Volume: 19 Pages: 340-342
  • Peer Reviewed
• [Journal Article] Glycyrrhizin attenuates mucus production by inhibition of MUC5AC mRNA expression in vivo and in vitro. (2010)
  • Author(s): Nishimoto Y(第一著者名)
  • Journal Title: J Pharmacol Sci Volume: 113 Pages: 76-83
  • Peer Reviewed
• [Journal Article] HSP70 inducers from Chinese herbs and their effect on melanin production. (2010)
  • Author(s): Yamashita Y(第一著者名)
  • Journal Title: Exp Dermatol Volume: 19 Pages: 340-342
  • Peer Reviewed
• [Journal Article] Synthesis and antiviral activities of some heteroaryl-substituted triarylmethanes. (2010)
  • Author(s): Mibu N(第一著者名)
  • Journal Title: J Heterocyclic Chem Volume: 47 Pages: 1434-1438
  • Peer Reviewed
• [Presentation] 新規対称性三置換1,3,5-トリアジン誘導体の合成と生物活性 (2013)
  • Author(s): 壬生 伸子
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜
• [Presentation] Fattiviracins, novel antiviral antibiotics effective for herpes simplex virus and human (2013)
  • Author(s): Kazumi Yokomizo
  • Organizer: FIP World Congress 2013
  • Place of Presentation: Dublin
• [Presentation] トリポーダル受容体型対称性分子の合成と生物活性 (2012)
  • Author(s): 壬生伸子
  • Organizer: 日本薬学会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-03-30
• [Presentation] トリポーダル受容体型対称性分子の合成と生物活性 (2012)
  • Author(s): 壬生伸子,横溝和美,植木菜美,斎藤愛,竹村知,内田航,周建融,安藤初美,宮田健,須本國弘
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌市
  • Year and Date: 2012-03-28
• [Presentation] Roles of antioxidants and heat shock protein induces as an antifatigue effects induced by health supplements (2010)
  • Author(s): 横溝和美 ,周建融,宮田健
  • Organizer: BIT'8th Annual Congress of InternationalDrug Discovery Sciences and Technology
  • Place of Presentation: 北京(中国).
  • Year and Date: 2010-10-26
• [Presentation] Molecular Pharmacological Evaluation of Maimendongtang, as an Example Strategy toward implementing Kampo into Modem Japon Medicine. (2010)
  • Author(s): 宮田健
  • Organizer: 5th International Congress of the International Society for History of Islamic Medicine
  • Place of Presentation: イスタンブール
  • Year and Date: 2010-10-26