Analysis of the mechanism of gastric tumorigenesis using mice with stomach-specific gene recombination

• Project/Area Number: 22590679
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: The University of Tokyo
• Principal Investigator: WATABE Hirotsugu 東京大学, 医学部附属病院, 臨床登録医 (50384764)
• Co-Investigator(Kenkyū-buntansha): HIRATA Yoshihiro 東京大学, 医学部附属病院, 助教 (10529192)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 癌 / シグナル伝達 / 胃癌 / マウスモデル / 細胞内シグナル

Research Abstract

The aim of this study was to establish stomach-specific gene recombination mice for the research of gastric tumorigenesis. Using this model, we established highly reproducible gastric tumor model and analyzed the mechanism of carcinogenesis. We found several cytokines and intracellular kinases were involved in the gastric carcinogenesis. Role of each molecule was clarified using knockout mice and gastric cancer cell lines treated with siRNA and inhibitors.

Research Products

• [Journal Article] Interleukin-6 mediates epithelial-stromal interactions and promotes gastric tumongenesis. (2013)
  • Author(s): Kinoshita H, et al.
  • Journal Title: PLoS One Volume: 12 Issue: 4 Pages: 1-10
  • DOI: 10.1371/journal.pone.0060914
  • Peer Reviewed
• [Journal Article] Apoptosis signal-regulating kinase-1 inhibitor as a potent therapeutic drug for the treatment of gastric cancer. (2012)
  • Author(s): Hayakawa, Y., Hirata, Y., Sakitani, K., Nakagawa, H., Nakata, W., Kinoshita, H., Takahashi, R., Takeda, K., Ichijo, H., Maeda1, S. and Koike, K.
  • Journal Title: Cancer Sci. Volume: 103 Issue: 12 Pages: 2181-2185
  • DOI: 10.1111/cas.12024
  • Peer Reviewed
• [Journal Article] Role of interleukin-32 in Helicobacter pylori-induced gastric inflammation. (2012)
  • Author(s): Kousuke Sakitani, et al.
  • Journal Title: Infection and Immunity Volume: 80 Issue: 11 Pages: 3795-3803
  • DOI: 10.1128/iai.00637-12
  • Peer Reviewed
• [Presentation] K19 positive cells are important progenitor of squamo-columnar junction tumor in mouse (2013)
  • Author(s): 平田喜裕
  • Organizer: 米国消化器病学会週間
  • Place of Presentation: Orlando, USA
  • Year and Date: 2013-05-19
• [Presentation] Increased interleukin-32 expression in gastric inflammation induced by Helicobacter pylori (2013)
  • Author(s): 崎谷康佑
  • Organizer: 第二回 日本消化器病学会国際カンファレンス
  • Place of Presentation: 鹿児島
  • Year and Date: 2013-03-23
• [Presentation] pylori感染によるROSを介したアポトーシス制御機構の解析 (2012)
  • Author(s): 平田喜裕、H.
  • Organizer: 日本消化器関連学会週間
  • Place of Presentation: 神戸
  • Year and Date: 2012-10-10
• [Presentation] Role of IL-32 in H. pylori induced gastric inflammation (2012)
  • Author(s): 崎谷康佑
  • Organizer: 米国消化器病学会週間
  • Place of Presentation: San Diego USA
  • Year and Date: 2012-05-20
• [Presentation] ASK1 plays a critical role in H.pylori induced gastric inflammation and metaplasia (2012)
  • Author(s): 早河翼
  • Organizer: 米国消化器病学会週間
  • Place of Presentation: San Diego USA
  • Year and Date: 2012-05-20
• [Presentation] 胃発癌におけるASK1の役割マウスモデルを用いた解析 (2011)
  • Author(s): 早河翼
  • Organizer: 日本消化器関連学会週間
  • Place of Presentation: 福岡
  • Year and Date: 2011-10-21
• [Presentation] 胃発癌におけるASK1の役割-マウスモデルを用いた解析 (2011)
  • Author(s): 早河翼
  • Organizer: 日本消化器関連学会週間
  • Place of Presentation: 福岡
  • Year and Date: 2011-10-21
• [Presentation] 胃癌におけるApoptosis Signal-regulating kinase1の重要性 (2010)
  • Author(s): 早河翼
  • Organizer: 日本癌学会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-22
• [Presentation] 胃癌におけるApoptosis Signal-regulating kinaselの重要性 (2010)
  • Author(s): 早河翼
  • Organizer: 日本癌学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-09-22
• [Presentation] H. pylori感染によるROSを介したアポトーシス制御機構の解析
  • Author(s): 平田 喜裕
  • Organizer: 日本消化器関連学会週間
  • Place of Presentation: 神戸
• [Presentation] ASK1 plays a critical role in H. pylori induced gastric inflammation and metaplasia.
  • Author(s): Hayakawa Y
  • Organizer: 米国消化器病学会週間
  • Place of Presentation: San Diego, U.S.A.
• [Presentation] Role of IL-32 in H. pylori induced gastric inflammation
  • Author(s): Sakitani K
  • Organizer: 米国消化器病学会週間
  • Place of Presentation: San Diego, U.S.A.
• [Presentation] Increased Interleukin-32 expression in gastric inflammation induced by Helicobacter pylori
  • Author(s): Sakitani K
  • Organizer: The 2nd JSGE international topic conference
  • Place of Presentation: 鹿児島
• [Presentation] K19 positive cells are important progenitor of squamo-olumnar junction tumor in mouse
  • Author(s): Hirata Y
  • Organizer: 米国消化器病学会週間
  • Place of Presentation: Orlando, U.S.A.