Functional analysis of tight junction protein: claudins on the development and progression of gastric cancer

• Project/Area Number: 22590691
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Hyogo Medical University
• Principal Investigator: OSHIMA Tadayuki 兵庫医科大学, 医学部, 講師 (00381814)
• Co-Investigator(Kenkyū-buntansha): WATARI Jiro 兵庫医科大学, 医学部, 教授 (10311531) ; MIWA Hirot 兵庫医科大学, 医学部, 教授 (80190833)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 上部消化管学(食道,胃,十二指腸) / 胃癌 / 細胞間接着装置 / タイト結合 / クローディン

Research Abstract

The morbidity of gastric cancer is still high in Japan, and it is know that Helicobacter pylori infection is involved in the development of gastric cancer. However, the incidence of gastric cancer in Helicobacter pylori infected people is a couple of %. We focused on tight junction proteins as the factor related to the development of gastric cancer and the malignancy. Down-regulation of claudin-3 was associated with proliferative potential of gastric cancer cells. Furthermore, down-regulation of claudin-18 at the invasive front of gastric cancer was associated with the proliferative and invasivepotential, suggesting that they have pivotal roles in gastric cancer progression.

Research Products

• [Journal Article] REG Iα is a biomarker for predicting response to chemotherapy S-1 plus cisplatin in patients with unresectab le stage IV gastric cancer. (2013)
  • Author(s): Sekikawa A, Fukui H, Zhang X, Maruo T, Tsumura T, Okabe Y, Wakasa T, Osaki Y, Chiba T, Tomita T, Oshima T, Watari J, Miwa H.
  • Journal Title: Br J Cancer. Volume: 108(2) Issue: 2 Pages: 395-401
  • DOI: 10.1038/bjc.2012.572
  • Peer Reviewed / Open Access
• [Journal Article] Down-regulation of claudin-3 is associated with proliferative potential in early gastric cancers. (2012)
  • Author(s): Okugawa T, Oshima T, Chen X, Hori K, Tomita T, Fukui H, Watari J, Matsumoto T, Miwa H
  • Journal Title: Dig Dis Sci. Volume: 57(6) Issue: 6 Pages: 1562-7
  • DOI: 10.1007/s10620-012-2043-5
  • Peer Reviewed
• [Journal Article] Biomarkers predicting development of metachronous gastric cancer after endoscopic resection: An analysis of molecular pathology of Helicobacter pylori eradication. (2012)
  • Author(s): Watari J, Moriichi K, Tanabe H, Kashima S, Nomura Y, Fujiya M, Tomita T, Oshima T, Fukui H, Miwa H, Das KM, Kohgo Y.
  • Journal Title: Int J Cancer. Volume: 15;130(10) Issue: 10 Pages: 2349-58
  • DOI: 10.1002/ijc.26275
  • Peer Reviewed
• [Journal Article] Outcomes of Treatment of Argon Plasma Coagulation Therapy in Elderly or High-risk Patients With Early Gastric Cancer: A Comparison of Outcomes Among Experienced and Nonexperienced Endoscopists. (2011)
  • Author(s): Tomita T, Arai E, Kohno T, Kondo T, Kim Y, Oshima T, Hori K, Watari J, Matsumoto T, Miwa H.
  • Journal Title: J Clin Gastroenterol. Volume: 45(6) Issue: 6 Pages: 54-9
  • DOI: 10.1097/mcg.0b013e3181ef3612
  • Peer Reviewed
• [Journal Article] Multiple gastric polypoid lesions with protein-losing enteropathy. (2011)
  • Author(s): Sakurai J, Watari J, Oshima T, Miwa H.
  • Journal Title: Intern Med. 査読有 Volume: 50(14) Pages: 1497-8
  • NAID: 130000969429
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21757837
  • Peer Reviewed
• [Presentation] Watari J, Fujimori T, Miwa H. Groαpromotes invasion of gastric cancer cells. (2013)
  • Author(s): Fukui H, Zhang X, Sun C, Yamasaki T, Kondo T, Ikehara H, Tomita T, Oshima T
  • Organizer: Sa1719 (Digestive Disease Week
  • Place of Presentation: Orlando, Florida, USA)
  • Year and Date: 2013-05-18
• [Presentation] Involvement of IL-22 signaling in gastric cancer cell invasion. (2012)
  • Author(s): Fukui H, Zhang X, Toyoshima F, TomitaT, Oshima T, Watari J, Imura J, Fujimori T, Matsumoto T, Miwa H.
  • Organizer: Su1840 (Digestive Disease Week
  • Place of Presentation: San Diego, California, USA)
  • Year and Date: 2012-05-22
• [Presentation] Down-regulation of submucosal claudin-18 at the invasive front is associated with proliferative potential of submucosal gastric cancer (2011)
  • Author(s): Okugawa T, Oshima T, et al
  • Organizer: DDW2011
  • Place of Presentation: Chicago, USA
  • Year and Date: 2011-05-09
• [Presentation] Down-regulation of submucosal claudin-18 at the invasivefront is associated with proliferative potential of submucosal gastric cancer. (2011)
  • Author(s): Okugawa T, Oshima T, Morita T, SakuraiJ, Tanaka J, Tomita T, Fukui H, Watari J, Matsumoto T, Miwa H.
  • Organizer: Sa1907 (Digestive Disease Week
  • Place of Presentation: Chicago, Illinois, USA)
  • Year and Date: 2011-05-07
• [Presentation] Implicationsin Gastrointestinal Diseases.(招聘講演) (2011)
  • Author(s): Oshima T. Tight Junctions
  • Organizer: The Sino-Japan Symposium aboutDigestive Diseases in Tianjin.
  • Place of Presentation: Tianjin, China
  • Year and Date: 2011-03-06
• [Presentation] 胃粘膜内癌におけるクローディンの発現と増殖能は相関する.奥川卓也,大島忠之 (2010)
  • Author(s): Chen Xin,神谷紀子,豊島史彦,櫻井淳, 田中淳二,森田毅,富田寿彦,堀和敏,渡 二郎,松本譽之,三輪洋人.
  • Organizer: JDDW2010一 般ポスター消P-159
  • Place of Presentation: 横浜,神奈川
  • Year and Date: 2010-10-13
• [Presentation] 胃粘膜内癌におけるクローディンの発現と増殖能は相関する (2010)
  • Author(s): 奥川卓也, 大島忠之, ら
  • Organizer: JDDW2010
  • Place of Presentation: 横浜
  • Year and Date: 2010-10-13
• [Presentation] Down-regulation of claudin-4 and up-regulation of claudin-18 are associated with proliferative potential of intramucosal gastric cancer. (2010)
  • Author(s): Chen X, Oshima T, Sakurai J, Tanaka J, Morita T, Tomita T, Kim Y, Hori K, Watari J, Matsumoto T, Miwa H.
  • Organizer: S1961 (Digestive Disease Week
  • Place of Presentation: New Orleans, Louisiana, USA)
  • Year and Date: 2010-05-02
• [Presentation] Down-regulation of claudin-4 and up-regulation of claudin-18 are associated with proliferative potential of intramucosal gastric cancer (2010)
  • Author(s): Chen X, Oshima T, et. al.
  • Organizer: DDW2010
  • Place of Presentation: New Orleans
  • Year and Date: 2010-05-02
• [Book] H.pylori除菌による早期胃癌内視鏡治療後の異時性発癌の抑制効果(解説/特集)消化器 内科(1884-2895)54 巻 3号Page302-308(2012.03) H.pyloriと胃癌の基礎と臨床
  • Author(s): 渡 二郎,盛一健太郎,田邊裕貴,藤谷幹浩,富田寿彦,大島忠之,福井広一,堀 和敏,松本譽之,三輪洋人,高後 裕.
  • Publisher: 科学評論社