| Project/Area Number |
22590694
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
ARAKI Akihiro 東京医科歯科大学, 医学部附属病院, 講師 (80361690)
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| Co-Investigator(Kenkyū-buntansha) |
TSUCHIYA Kichiro 東京医科歯科大学, 医歯学総合研究科, 講師 (40376786)
OKADA Eriko 東京医科歯科大学, 医学部附属病院, 助教 (20376784)
WATANABE Mamoru 東京医科歯科大学, 医歯学総合研究科, 教授 (10175127)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ダブルバルーン内視鏡 / DBE / 小腸生検 / Hath1 / Atoh1 / マッピング生検 / 小腸培養 / 杯細胞 / クローン病 / 小腸内視鏡 / 生検検体 |
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| Research Abstract |
The balloon endoscopy enables the collection of the biopsy specimen which reflected the entire small intestine environment in live. Therefore, we develop the differentiation mechanism of the intestinal epithelial cells to establish the assessment of whole small intestinal function in human. Then we finally aimed to consolidate a target of the treatment for small intestinal disease. In addition, I aimed to establish ex vivo culture of small intestinal epithelial cells to assess the function of the stem cells as a screening method.In this study, we compared the pathological findings between the patients with Crohn’s disease and healthy control. Interestingly, no change was shown in jejunum between the patients with Crohn’s disease and healthy control. However, comprehensive gene expression analysis with microarray showed the significant difference between the patients with Crohn’s disease and healthy control. Finally we identified specific genes in Japanese patients with Crohn’s disease.
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