• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Epigenetically regulated tissue repair mechanism in gastrointestinal tract

Research Project

Project/Area Number 22590712
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionResearch Institute, International Medical Center of Japan

Principal Investigator

KAWAMURA I. Yuki  独立行政法人国立国際医療研究センター, 肝炎・免疫研究センター・消化器疾患研究部・消化器病態生理研究室, 室長 (10392391)

Co-Investigator(Renkei-kenkyūsha) TOYOTA Minoru   (70270676)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords下部消化管学(小腸、大腸) / 糖鎖 / エピゲノム / 消化管
Research Abstract

In this study, we searched for the factors which promoted the regeneration of damaged gastrointestinal epithelium in the murine colitis model. We found that a group of genes involving in the differentiation to mucin-producing cells and the synthesis of mucins was DNA methylated and their expression were significantly decreased in regenerative epithelial cells isolated from the inflamed colon. Decreased expression of some human homologues was also observed in the ulcerative colitis. In addition, the DNA methylation level of the L-1 retrotransposon was dramatically decreased while the DNA methylation of genes involving in the transcription and the differentiation was accelerated in ulcerative colitis. These results suggest the importance of epigenetic gene regulation for the regeneration of the injured epithelium.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (35 results)

All 2012 2011 2010 Other

All Journal Article (14 results) (of which Peer Reviewed: 10 results) Presentation (21 results)

  • [Journal Article] Colonic epithelial cells express specific ligands for macrophage immunosuppressive receptors Siglec-7 and -92012

    • Author(s)
      Miyazaki K、Sakuma K、Kawamura YI、Izawa M、Ohmori K、Mitsuki M、Yamaji T、Hashimoto Y、Suzuki A、Saito Y、Dohi T、Kannagi R
    • Journal Title

      J Immunol

      Volume: 188(9) Pages: 4690-4700

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The monoclonal antibody HCM31 specifically recognizes the Sd^a tetrasaccharide in goblet cell mucin2012

    • Author(s)
      Tsubokawa D、Goso Y、Kawashima R、Ota H、Nakamura T、Nakamura K、Sato N、Kurihara M、Dohi T、Kawamura YI、Ichikawa T、Ishihara K
    • Journal Title

      FEBS Open Bio

      Volume: 2 Pages: 223-233

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comprehensive analysis of chemokines and cytokines secreted in the peritoneal cavity during laparotomy2012

    • Author(s)
      Kawashima R、Kawamura YI、Oshio T、Mizutani N、Okada T、Kawamura YJ、Konishi F、Dohi T
    • Journal Title

      Immunoassay Immunochem

      Volume: 33(3) Pages: 291-301

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Incomplete synthesis of carbohydrate determinants in ulcerative colitis2012

    • Author(s)
      Kawamura YI、Dohi T
    • Journal Title

      Nihon Rinsho

      Volume: 70(1) Pages: 646-650

    • NAID

      40019203412

    • Related Report
      2012 Final Research Report
  • [Journal Article] Colonic epithelial cells express specific ligands for mucosal macrophage immunosuppressive receptors, siglec-7 and-92012

    • Author(s)
      Miyazaki, K., Sakuma, K., Kawamura, Y. I., Izawa, M., Ohmori, K., Mitsuki, M., Yamaji, T., Hashimoto, Y., Suzuki, A., Saito, Y., Dohi, T., and Kannagi, R.
    • Journal Title

      J. Immunol

      Volume: 188 Issue: 9 Pages: 4690-4700

    • DOI

      10.4049/jimmunol.1100605

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The monoclonal antibody HCM31 specifically recognizes the Sda tetrasaccharide in goblet cell mucin2012

    • Author(s)
      Tsubokawa D, Goso Y, Kawashima R, Ota H, Nakamura T, Nakamura K, Sato N, Kurihara M, Dohi T, Kawamura YI, Ichikawa T, Ishihara K
    • Journal Title

      FEBS Open Bio

      Volume: 2 Issue: 1 Pages: 223-233

    • DOI

      10.1016/j.fob.2012.07.006

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comprehensive analysis of chemokines and cytokines secreted in the peritoneal cavity during laparotomy2012

    • Author(s)
      Kawashima R, Kawamura YI, Oshio T, Mizutani N, Okada T, Kawamura YJ, Konishi F, Dohi T
    • Journal Title

      J Immunoassay Immunochem

      Volume: 33 Issue: 3 Pages: 291-301

    • DOI

      10.1080/15321819.2011.638409

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Incomplete synthesis of carbohydrate determnants in ulcerative colitis2012

    • Author(s)
      Kawamura YI, Dohi T
    • Journal Title

      Nihon Rinsho

      Volume: 70 Pages: 646-650

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 潰瘍性大腸炎と糖鎖不全現象炎症性腸疾患-病因解明と診断・治療の最新知見-XII.特論2012

    • Author(s)
      河村由紀、土肥多恵子
    • Journal Title

      日本臨床

      Volume: 70 Pages: 646-650

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Interleukin-13 damages intestinal mucosa via TWEAK and Fn14 in mice - A pathway associated with ulcerative colitis2011

    • Author(s)
      Kawashima R、Kawamura YI、Oshio T、Son A、Yamazaki M、Hagiwara T、Okada T、Inagaki-Ohara K、Eu P、Szak S、Kawamura YJ、Konishi F、Miyake O、Yano H、Saito Y、Burkly LC、Dohi T
    • Journal Title

      Gastroenterology

      Volume: 141(6) Pages: 2119-2129

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The epithelia-specific membrane trafficking factor AP-1B secures gut immune homeostasis in mice2011

    • Author(s)
      Takahashi D、Hase K、Kimura S、Nakatsu F、Ohmae M、Mandai Y、Sato T、Date Y、Ebisawa M、Kato T、Obata Y、Kawamura YI、Dohi T、Katsuno T、Yokosuka O、Waguri S、Ohno H
    • Journal Title

      Gastroenterology

      Volume: 141(2) Pages: 621-632

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interleukin-13 damages intestinal mucosa via TWEAK and Fn14 in mice-A pathway associated with ulcerative colitis2011

    • Author(s)
      Kawashina R, Kawamura YI, Oshio T, Son A, Yamazaki M, Hagiwara T, Okada T, Inagaki-Ohara K, Eu P, Szak S, Kawamura YJ, Konishi F, Miyake O, Yano H, Saito Y, Burkly LC, Dohi T
    • Journal Title

      Gastroenterology

      Volume: 141 Pages: 2119-2129

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The epithelia-specific membrane trafficking factor AP-1B secures gut immune homeostasis in mice2011

    • Author(s)
      Takahashi D, Hase K, Kimura S, Nakatsu F, Ohmae M, Mandai Y, Sato T, Date Y, Ebisawa M, Kato T, Obata Y, Kawamura YI, Dohi T, Katsuno T, Yokosuka O, Waguri S, Ohno H
    • Journal Title

      Gastroenterology

      Volume: 141 Pages: 621-632

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 炎症-発癌と糖鎖不全現象2010

    • Author(s)
      河村由紀
    • Journal Title

      大腸癌フロンティア

      Volume: 3(3) Pages: 214-218

    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation] Genome-wide epigenomic analysis of lamina propria mononuclear cells isolated from patients with inflammatory bowel disease2012

    • Author(s)
      Kawamura YI、Otsubo T、Oshima K、Endo TA、Toyoda T、Hagiwara T、Kawamura YJ、Konishi F、Yano H、Saito Y、Hattori M、Dohi T
    • Organizer
      日米消化器病学会議
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] DNA hypermethylation of the Sd^a carbohydrate synthase gene as a possible biomarker for the prognosis of ulcerative colitis2012

    • Author(s)
      Sakurai T、Kawamura YI、Hagiwara T、Otsubo T、Hirano Maeyashiki C、Kojima Y、Gotoda T、Yano H、Saito Y、Kawamura YJ、Konishi F、Dohi T
    • Organizer
      米国消化器病週間
    • Place of Presentation
      サンディエゴ
    • Related Report
      2012 Final Research Report
  • [Presentation] Genome-wide epigenomic analysis of lamina propria mononuclear cells of inflammatory bowel disease2012

    • Author(s)
      TA、Toyoda T、Hagiwara T、Kawamura YJ、Konishi F、Yano H、Saito Y、Hattori M、Dohi T
    • Organizer
      米国消化器病週間
    • Place of Presentation
      サンディエゴ
    • Related Report
      2012 Final Research Report
  • [Presentation] Epigenomic analysis for small cell numbers of lamina propria T cells and macrophage/dendritic cell fraction in ulcerative colitis2012

    • Author(s)
      大坪武史、遠藤高帆、豊田哲郎、大島健志朗、萩原輝記、河村裕、小西文雄、矢野秀朗、斉藤幸夫、服部正平、稲垣匡子、河村由紀、土肥多惠子
    • Organizer
      日本生化学会
    • Place of Presentation
      福岡
    • Related Report
      2012 Final Research Report
  • [Presentation] 消化管で見られる癌性糖鎖不全とエピゲノム異常.ワークショップ2癌のepigeneticsとmicroRNA2011

    • Author(s)
      河村由紀
    • Organizer
      日本病態治療研究会
    • Place of Presentation
      東京(招待講演)
    • Year and Date
      2011-06-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] I.L-6, a Potent Inducer of DNA Hypermethylation and Malignant-Type Glycosylation in Ulcerative Colitis2011

    • Author(s)
      河村由紀
    • Organizer
      米国消化器病週間学会
    • Place of Presentation
      ニューオリンズ(米国)
    • Year and Date
      2011-05-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] IL-6, a potential inducer of DNA hypermethylation and malignant-type glycosylation in ulcerative colitis2011

    • Author(s)
      Kawamura YI、Toyota M、Hagiwara T、Suzuki H、Yamazaki M、Okada T、Kawamura YJ、Konishi F、Yano H、Saito Y、Dohi T
    • Organizer
      米国消化器病週間
    • Place of Presentation
      シカゴ
    • Related Report
      2012 Final Research Report
  • [Presentation] Establishment of a long term culture of self-replicating colon epithelial cells derived from a mouse model of inflammation-related tumor2011

    • Author(s)
      amazaki M、Kawamura YI、Okada T、Dohi T
    • Organizer
      米国消化器病週間
    • Place of Presentation
      シカゴ
    • Related Report
      2012 Final Research Report
  • [Presentation] 消化管でみられる癌性糖鎖不全とエピゲノム異常 ワークショップ2 癌のepigeneticsとmicroRNA2011

    • Author(s)
      河村由紀、豊田実、橋本真一、萩原輝記、山崎元美、河村裕、小西文雄、斉藤幸夫、服部正平、土肥多惠子
    • Organizer
      日本病態治療研究会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] パレット食道癌における粘液形質異常2010

    • Author(s)
      河村由紀
    • Organizer
      第52回日本消化器病学会大会
    • Place of Presentation
      横浜
    • Year and Date
      2010-10-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] IL-6はDNAメチル基転移酵素の異所性発現を介して潰瘍性大腸炎における糖鎖発現異常を誘導する2010

    • Author(s)
      河村由紀
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] IL-6 induces expression of DNA methyltransferases, gene silencing, and malignant-type glycosylation in ulcerative colitis and colitic cancer.2010

    • Author(s)
      河村由紀
    • Organizer
      米国消化器病週間学会
    • Place of Presentation
      ニューオリンズ(米国)
    • Year and Date
      2010-05-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] IL-6 induces expression of DNA methyltransferases, gene silencing, and malignant-type glycosylation in ulcerative colitis and colitic cancer2010

    • Author(s)
      Kawamura YI、Toyota M、Kawashima R、Okada T、Hagiwara T、Kawamura YJ、Konishi F、Matsumoto T、Dohi T
    • Organizer
      米国消化器病週間
    • Place of Presentation
      ニューオリンズ
    • Related Report
      2012 Final Research Report
  • [Presentation] IL-6誘導性DNA高メチル化がもたらす潰瘍性大腸炎およびcolitic cancerの糖鎖異常2010

    • Author(s)
      河村由紀、豊田実、川島麗、萩原輝記、河村裕、小西文雄、斉藤幸夫、松本誉之、神奈木玲児、今井浩三、土肥多惠子
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] バレット食道癌における粘液形質異常 ワークショップ22 Barrett食道のサーベイランス・治療を巡って2010

    • Author(s)
      河村由紀、秋山純一、土肥多惠子
    • Organizer
      日本消化器病学会
    • Place of Presentation
      横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] IL-6はDNAメチル基転移酵素の異所性発現を介して潰瘍性大腸炎における糖鎖異常を誘導する2010

    • Author(s)
      河村由紀、豊田実、Vongsavanh Phongsisay、岡田俊彦、山崎元美、河村裕、小西文雄、斉藤幸夫、鈴木拓、神奈木玲児、松本誉之、今井浩三、土肥多惠子
    • Organizer
      日本癌学会
    • Place of Presentation
      大阪
    • Related Report
      2012 Final Research Report
  • [Presentation] Genome-wide epigenomic analysis of lamina propria mononuclear cells isolated from patients with inflammatory bowel disease

    • Author(s)
      Kawamura YI, Otsubo T, Oshima K, Endo TA, Toyoda T, Hagiwara T, Kawamura YJ, Konishi F, Yano H, Saito Y, Hattori M, Dohi T
    • Organizer
      The 6th Japan & US Collaboration Conference in Gastroenterology
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] DNA hypermethylation of the Sda carbohydrate synthase gene as a possible biomarker for the prognosis of ulcerative colitis

    • Author(s)
      Sakurai T, Kawamura YI, Hagiwara T, Otsubo T, Hirano Maeyasiki C, Kojima Y, Gotoda T, Yano H, Saito Y, Kawamura YJ, Konishi F, Dohi T
    • Organizer
      Digestive Disease Week Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] IL-13 induces TWEAK-dependent activation of TNF-α and intestinal epithelial cell damage –a pathway associated with ulcerative colitis

    • Author(s)
      Dohi T, Kawashima R, Kawamura YI, Oshio T, Yamazaki M, Hagiwara T, Okada T, Wu P, Szak A, Kawamura YJ, Konishi F, Miyake O, Yano H, Saito Y, Burkly LC
    • Organizer
      Digestive Disease Week Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Genome-wide epigenomic analysis of lamina propria mononuclear cells of inflammatory bowel disease

    • Author(s)
      Otsubo T, Kawamura YI, Oshima K, Endo TA, Toyoda T, Hagiwara T, Kawamura YJ, Konishi F, Yano H, Saito Y, Hattori M, Dohi T
    • Organizer
      Digestive Disease Week Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] 末梢血白血球レクチン発現は炎症性腸疾患の病勢を反映するマーカーである

    • Author(s)
      前屋舗 千明、河村 由紀、萩原 輝記、大坪 武史、櫻井 俊之、永田 尚義、小島 康志、秋山 純一、神奈木 玲児、土肥 多恵子
    • Organizer
      第99回 日本消化器病学会総会
    • Place of Presentation
      城山観光ホテル・かごしま県民交流センター(鹿児島県)
    • Related Report
      2012 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi