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The mechanism of bile duct destruction in Primary Biliary Cirrhosis

Research Project

Project/Area Number 22590739
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionKyushu University

Principal Investigator

SHIMODA Shinji  九州大学, 大学病院, 講師 (30279319)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords原発生胆汁性肝硬変 / 自然免疫 / モデルマウス / 原発性胆汁性肝硬変 / NK細胞 / 胆管破壊 / 肝臓浸潤単核球 / 胆管上皮細胞 / TLR3リガンド / TLR4リガンド / 単球 / IFN-a
Research Abstract

Primary biliary cirrhosis (PBC) is characterized by chronic nonsuppurative destructive cholangitis (CNSDC) associated with destruction of small bile ducts. We examined the role of Toll-like receptors (TLRs), their ligands, and natural killer (NK) cells in modulating cytotoxic activity against biliary epithelial cells (BECs). We demonstrate that Toll-like receptor 4 ligand (TLR4-L)-stimulated NK cells destroy autologous BECs in the presence of interferon alpha (IFN-α) synthesized by TLR 3 ligand (TLR3-L)-stimulated monocytes (Mo). Indeed, IFN-α production by hepatic Mo is significantly increased in patients with PBC compared to disease controls. These data are supported by the immunohistochemical observation of an increased presence of CD56-positive NK cells scattered around destroyed small bile ducts more frequently in liver tissues from PBC patients than controls. To clarify the role of innate immune effector cells, such as natural killer (NK) cells, was studied in the mouse model based on the hypothesis that early events during immunization play an important role in the breakdown of tolerance. Following in-vivo depletion of NK cells, there is a marked suppression of anti-mitochondrial autoantibodies and cytokine production from autoreactive T cells. However, there was no change in the clinical pathology of portal inflammation compared to controls. These data support the hypothesis that there are probably multiple steps in the natural history of PBC, including a role of NK cells in initiating the breakdown of tolerance.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (39 results)

All 2012 2011 2010 Other

All Journal Article (22 results) (of which Peer Reviewed: 22 results) Presentation (16 results) Remarks (1 results)

  • [Journal Article] Vigorous cleaning and adequate ventilation are necessary to control an outbreak in a neonatal intensive care unit2012

    • Author(s)
      Shimono N, Hayashi J, Matsumoto H, Miyake N, Uchida Y, Shimoda S, Furusyo N, Akashi K
    • Journal Title

      J Infect Chemother

      Volume: 18(3) Pages: 303-7

    • NAID

      10030787099

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The role of natural killer (NK) and NK T cells in the loss of tolerance in murine primary biliary cirrhosis.2012

    • Author(s)
      Shimoda Shinji
    • Journal Title

      Clin Exp Immunol.

      Volume: Jun;168(3) Pages: 279-84

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Significance of immunoglobulin G4 (IgG4)-positive cells in extrahepatic cholangiocarcinoma: molecular mechanism of IgG4 reaction in cancer tissue.2012

    • Author(s)
      Harada Kenichi
    • Journal Title

      Hepatology

      Volume: Jul;56(1) Pages: 157-64

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genome-wide association study identifies TNFSF15 and POU2AF1 as susceptibility loci for primary biliary cirrhosis in the Japanese population.2012

    • Author(s)
      Nakamura Minoru
    • Journal Title

      Am J Hum Genet.

      Volume: Oct 5;91(4) Pages: 721-8

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fractalkine and other chemokines in primary biliary cirrhosis.2012

    • Author(s)
      Shimoda Shinji
    • Journal Title

      Int J Hepatol.

      Volume: 102839 Pages: 1-5

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic investigation of variably X chromosome inactivated genes in monozygotic female twins discordant for primary biliary cirrhosis2011

    • Author(s)
      Mitchell MM, Lleo A, Zammataro L, Mayo MJ, Invernizzi P, Bach N, Shimoda S, Gordon S, Podda M, Gershwin ME, Selmi C, LaSalle JM
    • Journal Title

      Epigenetics

      Volume: 6(1) Pages: 95-102

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Significance of periductal Langerhans cells and biliary epithelial cell-derived macrophage inflammatory protein-3a in the pathogenesis of primary biliary cirrhosis2011

    • Author(s)
      Harada K, Shimoda S, Ikeda H, Chiba M, Hsu M, Sato Y, Kobayashi M, Ren XS, Ohta H, Kasashima S, Kawashima A, Nakanuma Y
    • Journal Title

      Liver Int

      Volume: 31(2) Pages: 245-53

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intracellular B7-H4 suppresses bile duct epithelial cell apoptosis in human primary biliary cirrhosis2011

    • Author(s)
      Chen Y, Guo G, Guo S, Shimoda S, Shroyer KR, Tang Y, Wu Y
    • Journal Title

      Inflammation

      Volume: 34(6) Pages: 688-97

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Interaction between Toll-like receptors and natural killer cells in the destruction of bile ducts in primary biliary cirrhosis2011

    • Author(s)
      Shimoda S, Harada K, Niiro H, Shirabe K, Taketomi A, Maehara Y, Tsuneyama K, Nakanuma Y, Leung P, Ansari AA, Gershwin ME, Akashi K
    • Journal Title

      Hepatology

      Volume: 53(4) Pages: 1270-81

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Modulation of CD4 T cell responses following splenectomy in hepatitis C virus-related liver cirrhosis2011

    • Author(s)
      Hashimoto N, Shimoda S, Kawanaka H, Tsuneyama K, Uehara H, Akahoshi T, Kinjo N, Taketomi A, Shirabe K, Akashi K, Lleo A, Ansari AA, Gershwin ME, Maehara Y
    • Journal Title

      Clin Exp Immunol

      Volume: 165(2) Pages: 243-50

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fine phenotypic and functional characterization of effector cluster of differentiation 8 positive T cells in human patients with primary biliary cirrhosis2011

    • Author(s)
      Tsuda M, Ambrosini YM, Zhang W, Yang GX, Ando Y, Rong G, Tsuneyama K, Sumida K, Shimoda S, Bowlus CL, Leung PS, He XS, Coppel RL, Ansari AA, Lian ZX, Gershwin ME
    • Journal Title

      Hepatology

      Volume: 54(4) Pages: 1293-302

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Significance of periductal Langerhans cells and biliary epithelial cell-derived macrophage inflammatory protein-3α in the pathogenesis of primary biliary cirrhosis2011

    • Author(s)
      Harada K, Shimoda S, Ikeda H, Chiba M, Hsu M, Sato Y, Kobayashi M, Ren XS, Ohta H, Kasashima S, Kawashima A, Nakanuma Y
    • Journal Title

      Liver Int

      Volume: Feb;31(2) Pages: 245-53

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Modulation of CD4^+ T cell responses following splenectomy in hepatitis C virus-related liver cirrhosis2011

    • Author(s)
      Hashimoto N, Shimoda S, Kawanaka H, Tsuneyama K, Uehara H, Akahoshi T, Kinjo N, Taketomi A, Shirabe K, Akashi K, Lleo A, Ansari AA, Gershwin ME, Maehara Y
    • Journal Title

      Clin Exp Immunol

      Volume: Aug;165(2) Pages: 243-50

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epigenetic investigation of variably X chromosome inactivated genes in monozygotic female twins discordant for primary biliary cirrhosis2011

    • Author(s)
      Mitchell MM, Lleo A, Zammataro L, Mayo MJ, Invemizzi P, Bach N, Shimoda S, Gordon S, Podda M, Gershwin ME, Selmi C, LaSalle JM
    • Journal Title

      Epigenetics

      Volume: Jan;6(1) Pages: 95-102

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Significance of periductal Langerhans cells and biliary epithelial cell-derived macrophage inflammatory protein-3α in the pathogenesis of primary biliary cirrhosis.2011

    • Author(s)
      Harada K, Shimoda S, Ikeda H, Chiba M, Hsu M, Sato Y, Kobayashi M, Ren XS, Ohta H, Kasashima S, Kawashima A, Nakanuma Y.
    • Journal Title

      Liver Int.

      Volume: 31(2) Pages: 245-53

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Serum BAFF and APRIL levels in patients with PBC2010

    • Author(s)
      Migita K, Ilyassova B, Kovzel EF, Nersesov A, Abiru S, Maeda Y, Komori A, Ito M, Yano K, Yatsuhashi H, Shimoda S, Ishibashi H, Nakamura M
    • Journal Title

      Clin Immunol.Feb

      Volume: 134(2) Pages: 217-25

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] a signpost for biliary inflammation in primary biliary cirrhosis2010

    • Author(s)
      Shimoda S, Harada K, Niiro H, Taketomi A, Maehara Y, Tsuneyama K, Kikuchi K, Nakanuma Y, Mackay IR, Gershwin ME, Akashi K CX3CL1 (fractalkine)
    • Journal Title

      Hepatology. Feb

      Volume: 51(2) Pages: 567-75

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of HLA-DRB1 polymorphisms in Japanese patients with primary biliary cirrhosis (PBC) The HLA-DRB1polymorphism determines the relative risk of antinuclear antibodies for disease progression in PBC2010

    • Author(s)
      Nakamura M, Yasunami M, Kondo H, Horie H, Aiba Y, Komori A, Migita K, Yatsuhashi H, Ito M, Shimoda S, Ishibashi H; PBC Study Group in NHOSLJ
    • Journal Title

      Hepatol Res. May

      Volume: 40(5) Pages: 494-504

    • NAID

      10026392072

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Kyushu University Liver Disease Study Group.Pegylated interferon a-2b plus ribavirin for older patients with chronic hepatitis2010

    • Author(s)
      Kainuma M, Furusyo N, Kajiwara E, Takahashi K, Nomura H, Tanabe Y, Satoh T, Maruyama T, Nakamuta M, Kotoh K, Azuma K, Shimono J, Shimoda S, Hayashi J
    • Journal Title

      C. World J Gastroenterol

      Volume: 16(35) Pages: 4400-9

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] CX3CL1(fractalkine) : a signpost for biliary inflammation in primary biliary cirrhosis.2010

    • Author(s)
      Shimoda S, Harada K, Niiro H, Taketomi A, Maehara Y, Tsuneyama K, Kikuchi K, Nakanuma Y, Mackay IR, Gershwin ME, Akashi K.
    • Journal Title

      Hepatology

      Volume: Feb ; 51(2) Pages: 567-75

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Serum BAFF and APRIL levels in patients with PBC.2010

    • Author(s)
      Migita K, Ilyassova B, Kovzel EF, Nersesov A, Abiru S, Maeda Y, Komori A, Ito M, Yano K, Yatsuhashi H, Shimoda S, Ishibashi H, Nakamura M
    • Journal Title

      Clin Immunol

      Volume: Feb ; 134(2) Pages: 217-25

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of HLA-DRB1 polymorphisms in Japanese patients with primary biliary cirrhosis (PBC) : The HLA-DRB1 polymorphism determines the relative risk of antinuclear antibodies for disease progression in PBC.2010

    • Author(s)
      Nakamura M, Yasunami M, Kondo H, Horie H, Aiba Y, Komori A, Migita K, Yatsuhashi H, Ito M, Shimoda S, Ishibashi H ; PBC Study Group in NHOSLJ
    • Journal Title

      Hepatol Res

      Volume: May ; 40(5) Pages: 494-504

    • NAID

      10026392072

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 原発性胆汁性肝硬変における病因病態の解析2012

    • Organizer
      日本肝臓学会大会
    • Year and Date
      2012-10-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 原発性胆汁性肝硬変における胆管上皮の疾患特異的自己抗原発現条件の探索2012

    • Organizer
      日本肝臓学会総会
    • Year and Date
      2012-06-07
    • Related Report
      2012 Final Research Report
  • [Presentation] 原発性胆汁性肝硬変モデルマウスにおけるNK/NKT細胞の役割2012

    • Organizer
      日本消化器病学会
    • Year and Date
      2012-04-19
    • Related Report
      2012 Final Research Report
  • [Presentation] 原発性胆汁性肝硬変における胆管上皮の疾患特異的自己抗原発現の探索2012

    • Author(s)
      下田慎治
    • Organizer
      日本肝臓学会
    • Place of Presentation
      金沢
    • Related Report
      2012 Annual Research Report
  • [Presentation] 原発性胆汁性肝硬変モデルマウスにおけるNK/NKT細胞の役割2012

    • Author(s)
      下田慎治
    • Organizer
      日本消化器病学会総会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] 原発性胆汁性肝硬変における病因病態の解析2011

    • Author(s)
      下田慎治
    • Organizer
      日本肝臓学会大会
    • Place of Presentation
      福岡
    • Year and Date
      2011-10-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] 原発性胆汁性肝硬変における胆管破壊に関する新規知見2011

    • Organizer
      日本肝臓学会総会
    • Year and Date
      2011-06-02
    • Related Report
      2012 Final Research Report
  • [Presentation] 原発性胆汁性肝硬変における胆管破壊に関する新規知見2011

    • Author(s)
      下田慎治
    • Organizer
      日本肝臓学会総会
    • Place of Presentation
      東京
    • Year and Date
      2011-06-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] 原発性胆汁性肝硬変の病態解明と新規治療の可能性2011

    • Organizer
      日本消化器病学会
    • Year and Date
      2011-05-13
    • Related Report
      2012 Final Research Report
  • [Presentation] 原発性胆汁性肝硬変の病態解明と新規治療の可能性2011

    • Author(s)
      下田慎治
    • Organizer
      日本消化器病学会
    • Place of Presentation
      東京
    • Year and Date
      2011-05-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] 原発性胆汁性肝硬変(PBC)における免疫異常の基礎とそれに基づく予後予測について2010

    • Organizer
      日本肝臓学会大会
    • Year and Date
      2010-10-13
    • Related Report
      2012 Final Research Report
  • [Presentation] 原発性胆汁性肝硬変(PBC)における免疫異常の基礎とそれに基づく予後予測について2010

    • Author(s)
      下田慎治
    • Organizer
      JDDW 2010
    • Place of Presentation
      横浜
    • Year and Date
      2010-10-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] 原発性胆汁性肝硬変における胆管CX3CL1発現に必要なTNFaは、主にTLR4リガンドで単球から産生される2010

    • Organizer
      日本肝臓学会総会
    • Year and Date
      2010-05-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 原発性胆汁性肝硬変における胆管CX3CL1発現に必要なTNFαは、主にTLR4リガンドで単球から産生される2010

    • Author(s)
      下田慎治
    • Organizer
      第46回日本肝臓学会総会
    • Place of Presentation
      山形
    • Year and Date
      2010-05-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] 原発性胆汁性肝硬変における CX3CL1 産生の機序2010

    • Organizer
      日本消化器病学会総会
    • Year and Date
      2010-04-23
    • Related Report
      2012 Final Research Report
  • [Presentation] 原発性胆汁性肝硬変におけるCX3CL1産生の機序2010

    • Author(s)
      下田慎治
    • Organizer
      第96回日本消化器病学会総会
    • Place of Presentation
      新潟
    • Year and Date
      2010-04-23
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.1nai.med.kyushu-u.ac.jp/

    • Related Report
      2012 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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