The regulation mechanism of adipose tissue in survival, proliferation, differentiation and migration of hepatocellular carcinoma-derived cancer stem cells

• Project/Area Number: 22590740
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Saga University
• Principal Investigator: NISHIJIMA Aki (MATSUNOBU Aki) 佐賀大学, 医学部, 医員 (40566105)
• Co-Investigator(Kenkyū-buntansha): TODA Shuji 佐賀大学, 医学部, 教授 (80188755)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 肝癌幹細胞 / 脂肪組織 / 癌幹細胞-間質細胞相互作用 / 癌幹細胞ー間質細胞相互作用

Research Abstract

Adipose tissue inhibited the proliferation of hepatocellular carcinoma-derived cancer stem cells (CSCs) and did not affect apoptosis. The effect between subcutaneous and visceral adipose tissue about the migration is controversialimmunohistochemically, but there is no significant difference in the expression ofmigration-related molecules. Adipokine production levels differ between subcutaneous and visceral adipose tissue, but the production level is not different with or without CSCs.

Research Products

• [Journal Article] Three-dimensional culture model for analyzing crosstalk between adipose tissue and hepatocytes (2013)
  • Author(s): Nishijima-Matsunobu A, Uchihashi K, Aoki S, Ootani A, Node K, Toda S
  • Journal Title: Cell Tissue Res Volume: 352 Pages: 611-612
  • Peer Reviewed
• [Journal Article] Osteoblast migration into type I collagen gel and differentiation osteocyte-like cells within a self-produced mineralized matrix: A novel system for analyzing differentiation from osteoblast to osteocyte (2013)
  • Author(s): Uchihashi K, Aoki S, Matsunobu A, Ootani A, Node K, Toda S
  • Journal Title: Bone Volume: 52 (1) Pages: 102-110
  • Peer Reviewed
• [Journal Article] Adipose tissue behavior is distinctly regulated by neighboring cells and fluid flow stress: a possible role of adipose tissue in peritoneal fibrosis (2013)
  • Author(s): Aoki S, Udo K, Morimoto H, Ikeda S, Takezawa T, Uchihashi K,, Nishijima-Matsunobu A, Noguchi M, Toda S
  • Journal Title: Journal of Artifical Organs
  • Peer Reviewed
• [Journal Article] Three-Dimensional Culture Model for Analyzing Adipose Tissue–Hepatocyte Crosstalk (2013)
  • Author(s): Aki Nishijima-Matsunobu, Shigehisa Aoki, Kazuyoshi Uchihashi, Kazuma Fujimoto, and Shuji Toda
  • Journal Title: Cell and Tissue Research Volume: in press
  • Peer Reviewed
• [Journal Article] Interaction between adipose tissue stromal cells and gastric cancer cells in vitro (2011)
  • Author(s): Nomoto-Kojima N, Aoki S, Uchihashi K, Matsunobu A, Koike E, et. al.
  • Journal Title: Cell Tissue Res Volume: 344 Pages: 287-298
  • Peer Reviewed
• [Journal Article] A promising culture model for analying the interaction between adipose tissue and cardiomyocytes (2011)
  • Author(s): Anan M, Uchihashi K, Aoki S, Matsunobu A, Ootani A, Node K, Toda S
  • Journal Title: Endocrinology Volume: 152 (4) Pages: 1599-1605
  • Peer Reviewed
• [Journal Article] 脂肪組織と脂肪細胞の基礎形態学 (2011)
  • Author(s): 戸田修二、松延亜紀、内橋和芳、山本美保子、薬師寺舞、他4名
  • Journal Title: 臨床検査 Volume: 55 (6) Pages: 581-586
• [Journal Article] 脂肪組織を基盤とした生体恒常性・メタボリックシンドローム病態解析モデル (2011)
  • Author(s): 松延亜紀、藤本一眞、戸田修二
  • Journal Title: 臨床検査 Volume: 55 (6) Pages: 533-538
• [Journal Article] 脂肪組織を基盤とした生体恒常性・メタボリックシンドローム病態解析モデル (2011)
  • Author(s): 松延亜紀, 藤本一眞, 戸田修二
  • Journal Title: 臨床検査 Volume: 55 Pages: 581-586
• [Journal Article] 脂肪組織と脂肪細胞の基礎形態学 (2011)
  • Author(s): 戸田修二, 松延亜紀, 内橋和芳, 山本美保子, 薬師寺舞, 山崎文朗, 小池英介, 青木茂久, 杉原甫
  • Journal Title: 臨床検査 Volume: 55 Pages: 533-538
• [Journal Article] Adipose tissue explants and MDCK cells reciprocally regulate their morphogenesis in coculture (2010)
  • Author(s): Udo K, Aoki S, Uchihashi K, Kawasaki M, Matsunobu A, Tokuda Y, Ootani A, Toda S, Uozumi J.
  • Journal Title: Kidney Int Volume: 78 Pages: 60-68
  • Peer Reviewed
• [Journal Article] The air liquid-interface, a skin microenvironment, promotes growth of melanoma cells, but not their apoptosis and invasion, through activation of mitogen-activated protein kinase (2010)
  • Author(s): Yee CH, Aoki S, Uchihashi K, Matsunobu A,Yamasaki F, et.al.
  • Journal Title: Acta Histochem Cytochem Volume: 43 Pages: 1-7
  • NAID: 130000158695
  • Peer Reviewed
• [Journal Article] メタボリック症候群における脂肪組織の病理形態と病態解析培養モデル (2010)
  • Author(s): 戸田修二、青木茂久、内橋和芳、松延亜紀, 他5名
  • Journal Title: 病理と臨床 Volume: 28 (9) Pages: 959-965
• [Journal Article] Epidermal regeneration by keratinocyte-alien mesenchymal cell interactions (2010)
  • Author(s): Aoki S, Kitajima Y, Takezawa T, UchihashiK, Matsunobu A, et. al.
  • Journal Title: Inflammation and Regeneration Volume: 30 (5) Pages: 428-433
  • NAID: 10027651753
• [Presentation] 脂肪組織‐肝細胞相互作用解析モデルの確立 (2012)
  • Author(s): 松延 亜紀
  • Organizer: 日本内分泌病理学会学術総会
  • Place of Presentation: 仙台艮陵会館
  • Year and Date: 2012-10-12
• [Presentation] 内臓脂肪組織は肝細胞に脂肪毒性を誘導する (2012)
  • Author(s): 松延 亜紀
  • Organizer: 内臓脂肪組織は肝細胞に脂肪毒性を誘導する
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2012-04-27
• [Presentation] 脂肪組織が、肝細胞の生存・増殖・分化に及ぼす影響とその制御機構の解明 (2012)
  • Author(s): 松延 亜紀
  • Organizer: 日本内分泌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2012-04-21
• [Presentation] 脂肪組織由来間質細胞は胃癌の直接促進因子であり、分子標的療法の阻害因子である (2012)
  • Author(s): 松延 亜紀
  • Organizer: 日本消化管学会総会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2012-02-10
• [Presentation] 脂肪組織が、肝細胞の生存・増殖・分化に及ぼす影響とその制御機構 (2012)
  • Author(s): 松延亜紀、薬師寺舞、山本美保子、内橋和芳、青木茂久、米満伸久、戸田修二
  • Organizer: 日本内分泌学会学術総会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] 内臓脂肪組織は肝細胞に脂肪毒性を誘導する (2012)
  • Author(s): 松延亜紀、内橋和芳、青木茂久、山本美保子、薬師寺舞、山﨑文朗、米満伸久、戸田修二
  • Organizer: 日本病理学会総会
  • Place of Presentation: 京王プラザホテル
• [Presentation] 脂肪組織ー肝細胞相互作用解析モデルの確立 (2012)
  • Author(s): 松延亜紀、薬師寺舞、山本美保子、内橋和芳、青木茂久、米満伸久、戸田修二
  • Organizer: 日本内分泌病理学会学術総会
  • Place of Presentation: 艮陵会館(宮城県仙台市)
• [Presentation] A novel model for analyzing the interaction between adipose tissue and hepatocytes (2011)
  • Author(s): Matsunobu A, Uchihashi K, Aoki S, Toda S.
  • Organizer: 4thInternational Congress on PREDIABATES and the METABOLIC SYNDROME
  • Place of Presentation: Spain, Madrid
  • Year and Date: 2011-04-09
• [Presentation] A Novel Model for Analyzing the Interaction between Adipose Tissue and Hepatocyte (2011)
  • Author(s): 松延亜紀
  • Organizer: 4^<th> International Congress on Prediabetes and the Metabolic Syndrome
  • Place of Presentation: スペイン、マドリッド
  • Year and Date: 2011-04-09