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Vascular Biological Profile on Endothelial Progenitor Cell Origin and Differentiation

Research Project

Project/Area Number 22590796
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionTokai University

Principal Investigator

MASUDA Haruchika  東海大学, 医学部, 准教授 (50278496)

Co-Investigator(Renkei-kenkyūsha) ASAHARA Takayuki  東海大学, 医学部, 教授 (20246200)
Research Collaborator SHIZUNO Tomoko  東海大学, 医学部, 研究技術員
ITO Rie  東海大学, 医学部, 研究技術員
ITOH Johbu  東海大学, 医学部, 研究技術員
ITOH Yoshiko  東海大学, 医学部, 研究技術員
KAMIGUCHI Hiroshi  東海大学, 医学部, 研究技術員
OKADA Yoshinori  東海大学, 医学部, 研究技術員
IIDA Yumi  東海大学, 医学部, 研究技術員
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords血管内皮前駆細胞 / 血管再生 / 分化 / 造血幹細胞 / 分化動態解析法 / 血液幹細胞 / 血管生物学
Research Abstract

Circulating endothelial progenitor cell (EPC) is known to possess vascular repair/regeneration ability. However, the origin and differentiation cascade has yet to be elucidated. Our established analytical assay system of EPC expansion and differentiation revealed the functional features of EPC origin and differentiation. The findings provide us the potent information to develop diagnostic and therapeutic strategies for cardiovascular diseases.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (31 results)

All 2013 2012 2011 2010

All Journal Article (20 results) (of which Peer Reviewed: 19 results) Presentation (7 results) Book (2 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 2 results)

  • [Journal Article] Identification of mouse colony-forming endothelial progenitor cells for postnatal neovascularization: A novel insight highlighted by new mouse colony-forming assay2013

    • Author(s)
      Tsukada S, Masuda H, Asahara T
    • Journal Title

      Stem Cell Res Ther

      Volume: 4 Issue: 1 Pages: 20-20

    • DOI

      10.1186/scrt168

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Clonogenic assay of endothelial progenitor cells2013

    • Author(s)
      Masuda H, Asahara T
    • Journal Title

      Trends Cardiovasc Med

      Volume: 23 Issue: 4 Pages: 99-103

    • DOI

      10.1016/j.tcm.2012.09.007

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ex-vivo expanded human blood-derived CD133(+) cells promote repair of injured spinal cord2013

    • Author(s)
      Naosuke Kamei, Mitsuo Ochi
    • Journal Title

      Journal of the Neurological Sciences

      Volume: in press Issue: 1-2 Pages: 41-50

    • DOI

      10.1016/j.jns.2013.02.013

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Miyasaka M. Autologous g-csf mobilized peripheral blood cd34(+) cell therapy for diabetic patients with chronicnon-healing ulcer2012

    • Author(s)
      Tanaka R, Masuda H, Asahara T
    • Journal Title

      Cell Transplant

      Volume: 23 Issue: 2 Pages: 167-179

    • DOI

      10.3727/096368912x658007

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The therapeutic potential of ex vivo expanded CD133+ cells derived from human peripheral blood for peripheral nerve injuries2012

    • Author(s)
      Shin Ohtsubo, Mitsuo Ochi
    • Journal Title

      Journal of Neurosurgery

      Volume: 117 Issue: 4 Pages: 787-794

    • DOI

      10.3171/2012.7.jns111503

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fluid shear stress induces differentiation of circulating phenotype endothelial progenitor cells.2012

    • Author(s)
      S. Obi, H. Masuda, T. Shizuno, A. Sato, K. Yamamoto, J. Ando, Y. Abe and T. Asahara
    • Journal Title

      Am. J. Physiol. Cell Physiol.

      Volume: 303 Issue: 6 Pages: 595-606

    • DOI

      10.1152/ajpcell.00133.2012

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of serum-free quality and quantity control culture of colony-forming endothelial progenitor cell for vasculogenesis2012

    • Author(s)
      Masuda H, Asahara T
    • Journal Title

      Stem Cells Transl Med

      Volume: 1 Issue: 2 Pages: 160-171

    • DOI

      10.5966/sctm.2011-0023

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Local transplantation of ex vivo expanded bone marrow-derived cd34-positive cells accelerates fracture healing2012

    • Author(s)
      Kawakami Y, Masuda H, Asahara T
    • Journal Title

      Cell Transplant

      Volume: 21 Issue: 12 Pages: 2689-2709

    • DOI

      10.3727/096368912x654920

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Decursin inhibits vasculogenesis in early tumor progression by suppression of endothelial progenitor cell differentiation and function2012

    • Author(s)
      Jung SY, Masuda H, Asahara T
    • Journal Title

      J Cell Biochem

      Volume: 113 Issue: 5 Pages: 1478-1487

    • DOI

      10.1002/jcb.24085

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Up-regulation of mir-210 by vascular endothelial growth factor in ex vivo expanded cd34+ cells enhances cell-mediated angiogenesis2012

    • Author(s)
      Alaiti MA, Ishikawa M, Masuda H, Asahara T
    • Journal Title

      J Cell Mol Med.

      Volume: 16 Issue: 10 Pages: 2413-2421

    • DOI

      10.1111/j.1582-4934.2012.01557.x

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cd34+ cells represent highly functional endothelial progenitor cells in murine bone marrow2011

    • Author(s)
      Yang J, Masuda H, Asahara T
    • Journal Title

      PLoS One

      Volume: 6 Issue: 5 Pages: e20219-e20219

    • DOI

      10.1371/journal.pone.0020219

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impaired function of bone marrow-derived endothelial progenitor cells in murine liver fibrosis2011

    • Author(s)
      Shirakura K, Masuda H, Asahara T
    • Journal Title

      Biosci Trends

      Volume: 5 Pages: 77-82

    • NAID

      130005054590

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/21572251

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Aberrant kinetics of bone marrow-derived endothelial progenitor cells in the murine oxygen-induced retinopathy model2011

    • Author(s)
      Nakagawa Y, Masuda H, Asahara T
    • Journal Title

      Invest Ophthalmol Vis Sci

      Volume: 52 Issue: 11 Pages: 7835-7841

    • DOI

      10.1167/iovs.10-5880

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Methodological development of a clonogenic assay to determine endothelial progenitor cell potential2011

    • Author(s)
      Masuda H, Asahara T
    • Journal Title

      Circ Res

      Volume: 109 Issue: 1 Pages: 20-37

    • DOI

      10.1161/circresaha.110.231837

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential activity of bone marrow hematopoietic stem cell subpopulations for epc development and ischemic neovascularization2011

    • Author(s)
      Kwon SM, Masuda H, Asahara T
    • Journal Title

      J Mol Cell Cardiol

      Volume: 51 Issue: 3 Pages: 308-317

    • DOI

      10.1016/j.yjmcc.2011.04.007

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Concise review: Circulating endothelial progenitor cells for vascular medicine.2011

    • Author(s)
      Asahara T, Masuda H
    • Journal Title

      Stem Cells

      Volume: 29 Issue: 11 Pages: 1650-1655

    • DOI

      10.1002/stem.745

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of Serum-Free Quality and Quantity Control Culture of Colony Forming Endothelial Progenitor Cell for Vasculogenesis2011

    • Author(s)
      増田治史, 13人中1番目
    • Journal Title

      Stem Cells Translational Medicine

      Volume: 1巻 Pages: 160-171

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Concise review : Circulating endothelial progenitor cells for vascular medicine2011

    • Author(s)
      浅原孝之、川本篤彦、増田治史, 3人中3番目
    • Journal Title

      Stem Cells

      Volume: 29巻 Pages: 1650-1655

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impaired function of bone marrow-derived endothelial progenitor cells in murine liver fibrosis2011

    • Author(s)
      白倉克也, 等、増田治史, 9人中2番目
    • Journal Title

      Bioscience trends

      Volume: 5巻 Pages: 77-82

    • NAID

      130005054590

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 心血管再生の基礎研究2010

    • Author(s)
      増田治史、浅原孝之
    • Journal Title

      心臓(日本心臓財団)

      Volume: 42巻 Pages: 1564-1568

    • NAID

      130002147238

    • Related Report
      2010 Annual Research Report
  • [Presentation] Development of Serum-Free Suspension Culture System of Peripheral Blood Mononuclear Cells to Potentiate Vascular Regeneration2012

    • Author(s)
      増田治史
    • Organizer
      アメリカ心臓病学会2012
    • Place of Presentation
      ロスアンジェルスConvention Center、米国
    • Year and Date
      2012-11-07
    • Related Report
      2012 Final Research Report
  • [Presentation] Single cellによる造血幹細胞からの内皮系細胞系列決定、分化における細胞内情報伝達機構の解析2012

    • Author(s)
      増田治史
    • Organizer
      第10回日本再生医療学会総会
    • Place of Presentation
      京王プラザホテル
    • Year and Date
      2012-03-01
    • Related Report
      2012 Final Research Report
  • [Presentation] Development of Serum-Free Suspension Culture System of Peripheral Blood Mononuclear Cells to Potentiate Vascular Regeneration2012

    • Author(s)
      増田治史
    • Organizer
      American Heart Association Scientific Session
    • Place of Presentation
      Los Angels Convention Center, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Methodological Development of a Clonogenic Assay to Determine Endothelial Progenitor Cell Potential2011

    • Author(s)
      増田治史
    • Organizer
      第23回国際血栓止血学会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2011-07-26
    • Related Report
      2012 Final Research Report
  • [Presentation] Methodological Development of a Clonogenic Assay to Determine Endothelial Progenitor Cell Potential2011

    • Author(s)
      増田治史
    • Organizer
      第23回国際血栓止血学会
    • Place of Presentation
      京都国際会議場(京都府)
    • Year and Date
      2011-07-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Single cellによる造血幹細胞からの内皮系細胞系列決定、分化における細胞内情報伝達機構の解析2011

    • Author(s)
      増田治史
    • Organizer
      第10回日本再生医療学会
    • Place of Presentation
      京王プラザホテル
    • Related Report
      2010 Annual Research Report
  • [Presentation] Development of in vitro single cell based culture system to assess stem cell fate into endothelial lineage2010

    • Author(s)
      増田治史
    • Organizer
      第8回International Society of Stem Cell Research Conference
    • Place of Presentation
      サンフランシスコ
    • Related Report
      2010 Annual Research Report
  • [Book] 血管再生治療2012

    • Author(s)
      増田治史
    • Total Pages
      15
    • Publisher
      診断と治療社
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Book] 血管再生治療-現状から未来を展望する-2012

    • Author(s)
      増田治史
    • Total Pages
      15
    • Publisher
      診断と治療社
    • Related Report
      2011 Annual Research Report
  • [Patent(Industrial Property Rights)] 血管内皮前駆細胞を含む細胞群の生体外増幅方法2012

    • Inventor(s)
      増田治史
    • Industrial Property Rights Holder
      東海大学
    • Industrial Property Number
      2012-218206
    • Filing Date
      2012-09-28
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 血管内皮前駆細胞の生体外増幅法2012

    • Inventor(s)
      増田治史
    • Industrial Property Rights Holder
      東海大学
    • Acquisition Date
      2012-04-06
    • Related Report
      2012 Final Research Report
    • Overseas

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Published: 2010-08-23   Modified: 2019-07-29  

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