Phenotypic analyses for differentiationof human iPS cells into cardiomyocytes

• Project/Area Number: 22590813
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Keio University
• Principal Investigator: MURATA Mitsushige 慶應義塾大学, 医学部, 講師 (30317135)
• Co-Investigator(Kenkyū-buntansha): MAKINO Shinji 慶應義塾大学, 医学部, 特任准教授 (20306707)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: iPS 細胞 / 心筋細胞 / イオンチャネル / 心筋再生 / iPS細胞

Research Abstract

Human iPS-derived cardiomyocytes showed the nodal phenotypic features in molecular biological and electrophysiological analyses at the early period of differentiation, whereas they showed ventricular and atrial phenotypes at the late period. These results

Research Products

• [Journal Article] Distinct metabokic flow enables large-scale purification of pluripotent stem cell-derived cardiomyocytes. (2013)
  • Author(s): Tohyama S, Hattori F, Sano M, Hishiki T, Nagahata Y, Matsuura T, Yamashita H, Satoh Y, Hashimoto H, Egashira T, Seki T, Muraoka N, Yamakawa H, Ohgino Y, Yuasa S, Murata M, Suematsu M, Fukuda K.
  • Journal Title: Cell Stem Cells Volume: 12 Pages: 127-137
  • Peer Reviewed
• [Journal Article] Distinct metabolic flow enables large-scale purification of mouse and human pluripotent stem cell-derived cardiomyocytes. (2013)
  • Author(s): Shugo Tohyama
  • Journal Title: Cell Stem Cell Volume: 12 Pages: 127-137
  • Peer Reviewed
• [Journal Article] Effect of lesional differences in prolapsed leaflets on clinical outcomes in patients with mitral valve prolapse (2013)
  • Author(s): Keiko Suzuki
  • Journal Title: Am J Cardiovasc Dis Volume: 3 Pages: 152-159
  • Peer Reviewed
• [Journal Article] Disease characterization using LQTS-specific induced pluripotent stem cells. (2012)
  • Author(s): Egashira T, Yuasa S, Suzuki T, Aizawa Y, Yamakawa H, Matsuhashi T, Ohno Y, Tohyama S, Okata S, Seki T, Kuroda Y, Yae K, Hashimoto H, Tanaka T, Hattori F, Sato T, Miyoshi S, Takatsuki S, Murata M, Kurokawa J, Furukawa T, Makita N, Aiba T, Shimizu W, Horie
  • Journal Title: Cardiovasc Res Volume: 95 Pages: 419-429
  • Peer Reviewed
• [Journal Article] Effect of lesional differences in prolapsed leaflets on clinical outcomes in patients with mitral valve prolapse. (2012)
  • Author(s): Suzuki K, Murata M, Yasuda R, Tsuruta H, Tomotsugu N, Abe T, Iwanaga S, Akaishi M, Fukuda K.
  • Journal Title: Am J Cardiovasc Disease. Volume: 2 Pages: 152-159
  • Peer Reviewed
• [Journal Article] Frequent long-distance flyer's undersirable mileage: an organized giant thrombus stuck in PFO. (2012)
  • Author(s): Katsumata Y, Kimura K, Sano M, Kudo M, Arai T, Ohno Y, Tamura Y, Tsuruta H, Murata M, Yozu R, Fukuda K.
  • Journal Title: J Thromb Thrombolysis. Volume: 33 Pages: 296-298
  • Peer Reviewed
• [Journal Article] Significance of electrocardiographic right ventricular hypertrophy in patients with pulmonary hypertension or without right ventricular systolic dysfunction (2012)
  • Author(s): Nagai T
  • Journal Title: Intern Med Volume: 51 Pages: 2277-2283
  • Peer Reviewed
• [Journal Article] Disease characterization using LQTS-specific induced pluripotent stem cells. (2012)
  • Author(s): Egashira T, Yuasa S, Suzuki T, Tohyama S, et al.
  • Journal Title: Cardiovasc. Res. Volume: 95 Issue: 4 Pages: 419-29
  • DOI: 10.1093/cvr/cvs206
  • Peer Reviewed
• [Journal Article] Frequent long-distance flyer's undersirable mileage : an organized giant thrombus stuck in PFO (2012)
  • Author(s): Katsumata Y, et al
  • Journal Title: J Thromb Thrombolysis Volume: 33 Pages: 296-298
  • Peer Reviewed
• [Journal Article] Effect of lesional differences in prolapsed leaflets on clinical outcomes in patients with mitral valve prolapse (2012)
  • Author(s): Suzuki K, et al
  • Journal Title: Am J Cardiovasc Dis Volume: (In press)
  • Peer Reviewed
• [Journal Article] Significance of electrocardiographic right ventricular hypertrophy in patients with pulmonary hypertension or without right ventricular dysfunction. (2011)
  • Author(s): Nagai T, Kohsaka S, Murata M, Okuda S, Anzai T, Sato T, Fukuda K.
  • Journal Title: J Am Coll Cardiol Volume: 57
  • Peer Reviewed
• [Journal Article] Three-dimensional echocardiography findings of biventricular thrombi complicated by cerebral embolism. (2011)
  • Author(s): Egashira T, Murata M, Yasuda R, Suzuki K, Tsuruta H, Akaiashi M, Fukuda K.
  • Journal Title: J Echocardiography Volume: 9 Pages: 163-164
  • NAID: 10030334428
  • Peer Reviewed
• [Journal Article] Quantitative analysis of right ventricular function in patients with pulmonary hypertension using three-dimensional echocardiography and a two-dimensional summation method. (2011)
  • Author(s): Murata M, Morikawa T, Okuda S, Matsushita K, Iwanaga S, Murata M, Satoh T, Ogawa S, Fukuda K.
  • Journal Title: Am J Cardiol. Volume: 107 Pages: 484-489
  • Peer Reviewed
• [Journal Article] Impact of caloric restriction on cardiac senescence: Caloric restriction ameliorates cardiac diastolic dysfunction associated with aging. (2011)
  • Author(s): Shinmura K, Tamaki K, Sano M, Murata M, Yamakawa H, Fukuda K.
  • Journal Title: J Mol Cell Cardiol. Volume: 50 Pages: 117-127
  • Peer Reviewed
• [Journal Article] イオンチャネル蛋白の膜への輸送とその調節 (2011)
  • Author(s): 村田光繁, ほか
  • Journal Title: Japanese J.Electrocardiology Volume: 31 Pages: 3-12
• [Journal Article] Three-dimensional echocardiography findings of biventricular thrombi complicated by cerebral embolism (2011)
  • Author(s): Egashira T, et al
  • Journal Title: J Thromb Thrombolysis Volume: 9 Pages: 163-164
  • Peer Reviewed
• [Journal Article] Significance of electrocardiographic right ventricular hypertrophy in patients with pulmonary hypertension or without right ventricular dysfunction (2011)
  • Author(s): Nagai T, et al
  • Journal Title: J Am Coll Cardiol Volume: 57
  • Peer Reviewed
• [Journal Article] Quantitative analysis of right ventricular function in patients with pulmonary hypertension using three-dimensional echocardiography and a two-dimensional summation method (2011)
  • Author(s): Mitsushige Murata
  • Journal Title: Am J Cardiol Volume: 107 Pages: 484-489
  • Peer Reviewed
• [Journal Article] Zac1 is an essential transcription factor for cardiac morphogenesis. (2010)
  • Author(s): Yuasa S, Onizuka T, Shimoji K, Ohno Y, Kageyama T, Yoon SH, Egashira T, Seki T, Hasimoto H, Nishiyama T, Kaneda R, Murata M, Hattori F, Makino S, Sano M, Ogawa S, WJ Prall O, Harvey RP, Fukuda K.
  • Journal Title: Circ Res. Volume: 106 Pages: 1083-1091
  • Peer Reviewed
• [Journal Article] The impact of recent advances in cardiovascular regenerative medicine on clinical therapeutics and drug discovery. (2010)
  • Author(s): Murata M, Tohyama S, Fukuda K.
  • Journal Title: Pharmacology & Therapeutics Volume: 126 Pages: 109-118
  • Peer Reviewed
• [Journal Article] A novel in vivoDrosophia RNAi screen identifies NOT3 as a conserved regulator of heart function. (2010)
  • Author(s): Neely GG, Kuba K,Cammarato A, Isobe K, Amann S, Zhang L, Murata M, Elmen L, Gupta V, Arora S, Sarangi R, Dan D, Fujisawa S, Usami T, Xia CP, Keene AC, Alayari KN, Yamakawa H, Elling U, Berger C, Novatchkova M, Koglgruber R, Fukuda K, Nishina H, Isobe M, P
  • Journal Title: Cell Volume: 106 Pages: 142-153
  • Peer Reviewed
• [Journal Article] A non-genetic method for purifying stem cell-derived cardiomyocytes. (2010)
  • Author(s): Hattori F, Chen H, Yamashita H, Tohyama S, Satoh Y, Yuasa S, Li W, Yamakawa H, Tanaka T, Onitsuka T, Shimoji K, Ohno Y, Egashira T, Kaneda R, Murata M, Hidaka K, Morisaki T, Sasaki E, Suzuki T, Sano M, Makino S, Oikawa S, Fukuda K.
  • Journal Title: Nature Methods Volume: 7 Pages: 61-66
  • Peer Reviewed
• [Journal Article] The impact of recent advances in cardiovascular regenerative medicine on clinical therapeutics and drug discovery (2010)
  • Author(s): Mitsushige Murata
  • Journal Title: Pharmacology & Therapeutics Volume: 126 Pages: 109-118
  • Peer Reviewed
• [Journal Article] A novel in vivo Drosophia RNAi screen identifies NOT3 as a conserved regulator of heart function (2010)
  • Author(s): Neely GG, et al.
  • Journal Title: Cell Volume: 106 Pages: 142-153
  • Peer Reviewed
• [Presentation] Application of human iPS cell-technologies to arrhythmia researches (2011)
  • Author(s): Murata M
  • Organizer: the Asia Pacific Heart Rhythm Society Annual Meeting
  • Place of Presentation: 福岡福岡シーホーク
  • Year and Date: 2011-09-22
• [Presentation] Application of human iPS cell-technologies to arrhythmia researches. (2011)
  • Author(s): Murata M, Tohyama S, Kurokawa J, Egashira T, Yuasa S, Furukawa T, Fukuda K.
  • Organizer: The 4th the Asia Pacific Heart Rhythm Society Annual Meeting (Symposium)
  • Place of Presentation: Fukuoka
• [Presentation] Human peripheral T cell derived induced pluripotent stem cells can differentiate into functional cardiomyocyte (2011)
  • Author(s): Seki T, Yuasa S, Oda M, Egashira T, Yae K, Kusumoto D, Nakata H, Tohyama S, Hashimoto H, Kodaira M, Kuroda Y, Tanaka A, Ohkata S, Ohno Y, Murata M, Okada Y, Seimiya H, Fudaki N, Hasegawa M, Fukuda K.
  • Organizer: 第75回日本循環器学会学術集会
  • Place of Presentation: 横浜
• [Presentation] Suppression of Rad activity regulates L-type Ca^<2+> channels and causes triggered activities in mouse hearts (2010)
  • Author(s): Suzuki T, et al
  • Organizer: American Heart Association scientific session
  • Place of Presentation: 米国シカゴ
  • Year and Date: 2010-11-14
• [Presentation] 心筋イオンチャネルの膜への輸送とその調節 (2010)
  • Author(s): 村田光繁
  • Organizer: 第25回犬山不整脈カンファレンス(招待講演)
  • Place of Presentation: 名古屋
  • Year and Date: 2010-08-21
• [Presentation] 低分子量G蛋白質Radの抑制は、マウス心筋L型カルシウム電流を増加させることでtriggered activityを引き起こす (2010)
  • Author(s): 鈴木智之,村田 光繁,山川裕之、水澤美香、遠山周吾、扇野泰行、湯浅慎介、牧野伸司、佐野元昭,神谷香一郎、福田 恵一
  • Organizer: 平成22年度生理学研究所研究会「イオンチャネル・トランスポーターと心血管機能:細胞機能の分子機序とその統合的理解」
  • Place of Presentation: 岡崎
• [Presentation] ゲノムワイドRNAi心不全スクリーニングによる心機能調節因子CCR4-NOT複合体の同定 (2010)
  • Author(s): 久場敬司,鈴木亨、森田正行、村田光繁,山川裕之、Neely G、Penninger J、福田恵一、磯部光章、木村彰方、今井由美子
  • Organizer: 平成22年度生理学研究所研究会「イオンチャネル・トランスポーターと心血管機能:細胞機能の分子機序とその統合的理解」
  • Place of Presentation: 岡崎
• [Presentation] ヒトiPS由来心筋細胞の電気生理学的特性について (2010)
  • Author(s): 遠山周吾、村田光繁、黒川洵子、Lopez-Redondo F、服部文幸、水澤美加、山川裕之、橋本寿之、江頭徹、関朋久、扇野泰行、八戸宏二郎、湯浅慎介、福田恵一
  • Organizer: 第25回日本心電学会学術集会
  • Place of Presentation: 大分