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Phenotypic analyses for differentiationof human iPS cells into cardiomyocytes

Research Project

Project/Area Number 22590813
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionKeio University

Principal Investigator

MURATA Mitsushige  慶應義塾大学, 医学部, 講師 (30317135)

Co-Investigator(Kenkyū-buntansha) MAKINO Shinji  慶應義塾大学, 医学部, 特任准教授 (20306707)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
KeywordsiPS 細胞 / 心筋細胞 / イオンチャネル / 心筋再生 / iPS細胞
Research Abstract

Human iPS-derived cardiomyocytes showed the nodal phenotypic features in molecular biological and electrophysiological analyses at the early period of differentiation, whereas they showed ventricular and atrial phenotypes at the late period. These results

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (32 results)

All 2013 2012 2011 2010

All Journal Article (24 results) (of which Peer Reviewed: 23 results) Presentation (8 results)

  • [Journal Article] Distinct metabokic flow enables large-scale purification of pluripotent stem cell-derived cardiomyocytes.2013

    • Author(s)
      Tohyama S, Hattori F, Sano M, Hishiki T, Nagahata Y, Matsuura T, Yamashita H, Satoh Y, Hashimoto H, Egashira T, Seki T, Muraoka N, Yamakawa H, Ohgino Y, Yuasa S, Murata M, Suematsu M, Fukuda K.
    • Journal Title

      Cell Stem Cells

      Volume: 12 Pages: 127-137

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Distinct metabolic flow enables large-scale purification of mouse and human pluripotent stem cell-derived cardiomyocytes.2013

    • Author(s)
      Shugo Tohyama
    • Journal Title

      Cell Stem Cell

      Volume: 12 Pages: 127-137

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of lesional differences in prolapsed leaflets on clinical outcomes in patients with mitral valve prolapse2013

    • Author(s)
      Keiko Suzuki
    • Journal Title

      Am J Cardiovasc Dis

      Volume: 3 Pages: 152-159

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Disease characterization using LQTS-specific induced pluripotent stem cells.2012

    • Author(s)
      Egashira T, Yuasa S, Suzuki T, Aizawa Y, Yamakawa H, Matsuhashi T, Ohno Y, Tohyama S, Okata S, Seki T, Kuroda Y, Yae K, Hashimoto H, Tanaka T, Hattori F, Sato T, Miyoshi S, Takatsuki S, Murata M, Kurokawa J, Furukawa T, Makita N, Aiba T, Shimizu W, Horie
    • Journal Title

      Cardiovasc Res

      Volume: 95 Pages: 419-429

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of lesional differences in prolapsed leaflets on clinical outcomes in patients with mitral valve prolapse.2012

    • Author(s)
      Suzuki K, Murata M, Yasuda R, Tsuruta H, Tomotsugu N, Abe T, Iwanaga S, Akaishi M, Fukuda K.
    • Journal Title

      Am J Cardiovasc Disease.

      Volume: 2 Pages: 152-159

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Frequent long-distance flyer's undersirable mileage: an organized giant thrombus stuck in PFO.2012

    • Author(s)
      Katsumata Y, Kimura K, Sano M, Kudo M, Arai T, Ohno Y, Tamura Y, Tsuruta H, Murata M, Yozu R, Fukuda K.
    • Journal Title

      J Thromb Thrombolysis.

      Volume: 33 Pages: 296-298

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Significance of electrocardiographic right ventricular hypertrophy in patients with pulmonary hypertension or without right ventricular systolic dysfunction2012

    • Author(s)
      Nagai T
    • Journal Title

      Intern Med

      Volume: 51 Pages: 2277-2283

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Disease characterization using LQTS-specific induced pluripotent stem cells.2012

    • Author(s)
      Egashira T, Yuasa S, Suzuki T, Tohyama S, et al.
    • Journal Title

      Cardiovasc. Res.

      Volume: 95 Issue: 4 Pages: 419-29

    • DOI

      10.1093/cvr/cvs206

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Frequent long-distance flyer's undersirable mileage : an organized giant thrombus stuck in PFO2012

    • Author(s)
      Katsumata Y, et al
    • Journal Title

      J Thromb Thrombolysis

      Volume: 33 Pages: 296-298

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of lesional differences in prolapsed leaflets on clinical outcomes in patients with mitral valve prolapse2012

    • Author(s)
      Suzuki K, et al
    • Journal Title

      Am J Cardiovasc Dis

      Volume: (In press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Significance of electrocardiographic right ventricular hypertrophy in patients with pulmonary hypertension or without right ventricular dysfunction.2011

    • Author(s)
      Nagai T, Kohsaka S, Murata M, Okuda S, Anzai T, Sato T, Fukuda K.
    • Journal Title

      J Am Coll Cardiol

      Volume: 57

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional echocardiography findings of biventricular thrombi complicated by cerebral embolism.2011

    • Author(s)
      Egashira T, Murata M, Yasuda R, Suzuki K, Tsuruta H, Akaiashi M, Fukuda K.
    • Journal Title

      J Echocardiography

      Volume: 9 Pages: 163-164

    • NAID

      10030334428

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative analysis of right ventricular function in patients with pulmonary hypertension using three-dimensional echocardiography and a two-dimensional summation method.2011

    • Author(s)
      Murata M, Morikawa T, Okuda S, Matsushita K, Iwanaga S, Murata M, Satoh T, Ogawa S, Fukuda K.
    • Journal Title

      Am J Cardiol.

      Volume: 107 Pages: 484-489

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Impact of caloric restriction on cardiac senescence: Caloric restriction ameliorates cardiac diastolic dysfunction associated with aging.2011

    • Author(s)
      Shinmura K, Tamaki K, Sano M, Murata M, Yamakawa H, Fukuda K.
    • Journal Title

      J Mol Cell Cardiol.

      Volume: 50 Pages: 117-127

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] イオンチャネル蛋白の膜への輸送とその調節2011

    • Author(s)
      村田光繁, ほか
    • Journal Title

      Japanese J.Electrocardiology

      Volume: 31 Pages: 3-12

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Three-dimensional echocardiography findings of biventricular thrombi complicated by cerebral embolism2011

    • Author(s)
      Egashira T, et al
    • Journal Title

      J Thromb Thrombolysis

      Volume: 9 Pages: 163-164

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Significance of electrocardiographic right ventricular hypertrophy in patients with pulmonary hypertension or without right ventricular dysfunction2011

    • Author(s)
      Nagai T, et al
    • Journal Title

      J Am Coll Cardiol

      Volume: 57

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative analysis of right ventricular function in patients with pulmonary hypertension using three-dimensional echocardiography and a two-dimensional summation method2011

    • Author(s)
      Mitsushige Murata
    • Journal Title

      Am J Cardiol

      Volume: 107 Pages: 484-489

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Zac1 is an essential transcription factor for cardiac morphogenesis.2010

    • Author(s)
      Yuasa S, Onizuka T, Shimoji K, Ohno Y, Kageyama T, Yoon SH, Egashira T, Seki T, Hasimoto H, Nishiyama T, Kaneda R, Murata M, Hattori F, Makino S, Sano M, Ogawa S, WJ Prall O, Harvey RP, Fukuda K.
    • Journal Title

      Circ Res.

      Volume: 106 Pages: 1083-1091

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The impact of recent advances in cardiovascular regenerative medicine on clinical therapeutics and drug discovery.2010

    • Author(s)
      Murata M, Tohyama S, Fukuda K.
    • Journal Title

      Pharmacology & Therapeutics

      Volume: 126 Pages: 109-118

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel in vivoDrosophia RNAi screen identifies NOT3 as a conserved regulator of heart function.2010

    • Author(s)
      Neely GG, Kuba K,Cammarato A, Isobe K, Amann S, Zhang L, Murata M, Elmen L, Gupta V, Arora S, Sarangi R, Dan D, Fujisawa S, Usami T, Xia CP, Keene AC, Alayari KN, Yamakawa H, Elling U, Berger C, Novatchkova M, Koglgruber R, Fukuda K, Nishina H, Isobe M, P
    • Journal Title

      Cell

      Volume: 106 Pages: 142-153

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A non-genetic method for purifying stem cell-derived cardiomyocytes.2010

    • Author(s)
      Hattori F, Chen H, Yamashita H, Tohyama S, Satoh Y, Yuasa S, Li W, Yamakawa H, Tanaka T, Onitsuka T, Shimoji K, Ohno Y, Egashira T, Kaneda R, Murata M, Hidaka K, Morisaki T, Sasaki E, Suzuki T, Sano M, Makino S, Oikawa S, Fukuda K.
    • Journal Title

      Nature Methods

      Volume: 7 Pages: 61-66

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The impact of recent advances in cardiovascular regenerative medicine on clinical therapeutics and drug discovery2010

    • Author(s)
      Mitsushige Murata
    • Journal Title

      Pharmacology & Therapeutics

      Volume: 126 Pages: 109-118

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel in vivo Drosophia RNAi screen identifies NOT3 as a conserved regulator of heart function2010

    • Author(s)
      Neely GG, et al.
    • Journal Title

      Cell

      Volume: 106 Pages: 142-153

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Application of human iPS cell-technologies to arrhythmia researches2011

    • Author(s)
      Murata M
    • Organizer
      the Asia Pacific Heart Rhythm Society Annual Meeting
    • Place of Presentation
      福岡福岡シーホーク
    • Year and Date
      2011-09-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] Application of human iPS cell-technologies to arrhythmia researches.2011

    • Author(s)
      Murata M, Tohyama S, Kurokawa J, Egashira T, Yuasa S, Furukawa T, Fukuda K.
    • Organizer
      The 4th the Asia Pacific Heart Rhythm Society Annual Meeting (Symposium)
    • Place of Presentation
      Fukuoka
    • Related Report
      2012 Final Research Report
  • [Presentation] Human peripheral T cell derived induced pluripotent stem cells can differentiate into functional cardiomyocyte2011

    • Author(s)
      Seki T, Yuasa S, Oda M, Egashira T, Yae K, Kusumoto D, Nakata H, Tohyama S, Hashimoto H, Kodaira M, Kuroda Y, Tanaka A, Ohkata S, Ohno Y, Murata M, Okada Y, Seimiya H, Fudaki N, Hasegawa M, Fukuda K.
    • Organizer
      第75回日本循環器学会学術集会
    • Place of Presentation
      横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] Suppression of Rad activity regulates L-type Ca^<2+> channels and causes triggered activities in mouse hearts2010

    • Author(s)
      Suzuki T, et al
    • Organizer
      American Heart Association scientific session
    • Place of Presentation
      米国シカゴ
    • Year and Date
      2010-11-14
    • Related Report
      2010 Annual Research Report
  • [Presentation] 心筋イオンチャネルの膜への輸送とその調節2010

    • Author(s)
      村田光繁
    • Organizer
      第25回犬山不整脈カンファレンス(招待講演)
    • Place of Presentation
      名古屋
    • Year and Date
      2010-08-21
    • Related Report
      2012 Final Research Report
  • [Presentation] 低分子量G蛋白質Radの抑制は、マウス心筋L型カルシウム電流を増加させることでtriggered activityを引き起こす2010

    • Author(s)
      鈴木智之,村田 光繁,山川裕之、水澤美香、遠山周吾、扇野泰行、湯浅慎介、牧野伸司、佐野元昭,神谷香一郎、福田 恵一
    • Organizer
      平成22年度生理学研究所研究会「イオンチャネル・トランスポーターと心血管機能:細胞機能の分子機序とその統合的理解」
    • Place of Presentation
      岡崎
    • Related Report
      2012 Final Research Report
  • [Presentation] ゲノムワイドRNAi心不全スクリーニングによる心機能調節因子CCR4-NOT複合体の同定2010

    • Author(s)
      久場敬司,鈴木亨、森田正行、村田光繁,山川裕之、Neely G、Penninger J、福田恵一、磯部光章、木村彰方、今井由美子
    • Organizer
      平成22年度生理学研究所研究会「イオンチャネル・トランスポーターと心血管機能:細胞機能の分子機序とその統合的理解」
    • Place of Presentation
      岡崎
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒトiPS由来心筋細胞の電気生理学的特性について2010

    • Author(s)
      遠山周吾、村田光繁、黒川洵子、Lopez-Redondo F、服部文幸、水澤美加、山川裕之、橋本寿之、江頭徹、関朋久、扇野泰行、八戸宏二郎、湯浅慎介、福田恵一
    • Organizer
      第25回日本心電学会学術集会
    • Place of Presentation
      大分
    • Related Report
      2012 Final Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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