molecular signature for detection of intractable tumors

• Project/Area Number: 22590858
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Nagoya University
• Principal Investigator: YANAGISAWA Kiyoshi 名古屋大学, 医学系研究科, 講師 (20372112)
• Co-Investigator(Kenkyū-buntansha): ARIMA Chinatsu 名古屋大学, 大学院・医学系研究科, 特任助教 (00534843)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 悪性胸膜中皮腫 / 肺癌 / プロテオミクス / プロテオーム / 非閉塞性肺疾患

Research Abstract

We employed shotgun proteomics together with peptide labeling technology to analyze comprehensive protein expression profiles of malignant pulmonary mesothelioma (MPM) and lung cancer and hundreds of candidate marker protein have been identified. Thus, our findings demonstrate that the combined proteomic technology is an efficient strategy for global proteomic analysis to discover novel biomarkers. We have applied two patents according to our observations.

Research Products

• [Journal Article] MYBPH inhibits NM IIA assembly via direct interaction with NMHC IIA and reduces cell motility (2012)
  • Author(s): Hosono Y, Yanagisawa K, and Takahashi T et al.
  • Journal Title: Biochem Biophys Res Commun Volume: 428 Pages: 173-8
  • Peer Reviewed
• [Journal Article] Seven-Signal Proteomic Signature for Detection of Operable Pancreatic Ductal Adenocarcinoma and Their Discrimination from Autoimmune Pancreatitis (2012)
  • Author(s): Yanagisawa K, Arima C, and Takahashi T et al.
  • Journal Title: International Journal of Proteomics Volume: 2012 Pages: 510397-510397
  • Peer Reviewed
• [Journal Article] NKX2-1/TITF1/TTF-1-induced ROR1 is required to sustain EGFR survival signaling in lung adenocarcinoma (2012)
  • Author(s): Yamaguchi T, Yanagisawa K, Arima C, and Takahashi T et al.
  • Journal Title: Cancer Cell Volume: 21 Pages: 348-61
  • Peer Reviewed
• [Journal Article] MYBPH inhibits NM IIA assembly via direct interaction with NMHC IIA and reduces cell motility. (2012)
  • Author(s): Hosono Y
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 428 Issue: 1 Pages: 173-178
  • DOI: 10.1016/j.bbrc.2012.10.036
  • Peer Reviewed
• [Journal Article] Seven-signal proteomic signature for detection of operable pancreatic cancer and their discrimination from autoimmune pancreatitis (2012)
  • Author(s): Yanagisawa K
  • Journal Title: Int J Proteomics Volume: 2012 Pages: 1-11
  • DOI: 10.1155/2012/510397
  • Peer Reviewed
• [Journal Article] MYBPH, a transcriptional target of TTF-1, inhibits ROCK1, and reduces cell motility and metastasis (2011)
  • Author(s): Hosono Y, Yanagisawa K, Arima C, and Takahashi T et al
  • Journal Title: EMBO journal Volume: 31 Pages: 481-93
  • Peer Reviewed
• [Journal Article] miR-375 is activated by ASH1 and inhibits YAP1 in a lineage-dependent manner in lung cancer. (2011)
  • Author(s): Nishikawa E, Arima C, Yanagisawa K, and Takahashi T et al
  • Journal Title: Cancer Research Volume: 71 Pages: 6165-73
  • Peer Reviewed
• [Journal Article] MYBPH, a transcriptional target of TTF-1, inhibits ROCK1, and reduces cell motility and metastasis (2011)
  • Author(s): Hosono Y., et al
  • Journal Title: EMBO Journal Volume: 31 Pages: 481-93
  • Peer Reviewed
• [Journal Article] miR-375 is activated by ASH1 and inhibits YAP1 in a lineage-dependent manner in lung cancer (2011)
  • Author(s): Nishikawa E., et al
  • Journal Title: Cancer Research Volume: 71 Pages: 6165-73
  • Peer Reviewed
• [Journal Article] Novel metastasis-related gene CIM functions in the regulation of multiple cellular stress-respnse pathways. (2010)
  • Author(s): Yanagisawa K, Arima C, and Takahashi T et al
  • Journal Title: Cancer Research Volume: 70 Pages: 9949-58
  • Peer Reviewed
• [Journal Article] Novel metastasis-related gene CIM functions in the regulation of multiple cellular stress response pathways (2010)
  • Author(s): Yanagisawa K
  • Journal Title: Cancer Research Volume: 709 Pages: 9949-9958
  • Peer Reviewed
• [Journal Article] Regulation of DNA polymerase POLD4 influences genomic instability in lung cacner (2010)
  • Author(s): Huang QM
  • Journal Title: Cancer Research Volume: 70 Pages: 8407-8416
  • Peer Reviewed
• [Journal Article] Clinically relevant characterization of lung adenocarcinoma subtypes based on cellular pathways : an international validation study (2010)
  • Author(s): Bryant CM
  • Journal Title: PLoS One Volume: 50
  • Peer Reviewed
• [Journal Article] Seven-Signal Proteomic Signature for Detection of Operable Pancreatic Ductal Adenocarcinoma and Their Discrimination from Autoimmune Pancreatitis
  • Author(s): Yanagisawa K., et al
  • Journal Title: International Journal of Proteomics Volume: (in press)
  • Peer Reviewed
• [Journal Article] NKX2-1/TITF1/TTF-1-induced ROR1 is required to sustain EGFR survival signaling in lung adenocarcinoma
  • Author(s): Yamaguchi T., et al
  • Journal Title: Cancer Cell Volume: (in press)
  • Peer Reviewed
• [Presentation] Proteomic identification of potential biomarkers of malignant pulmonary mesothelioma (2012)
  • Author(s): Yanagisawa K et a
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌、札幌
  • Year and Date: 2012-09-21
• [Presentation] Quantitative Proteomic Profiling Identifies CKAP4 as a MalignantPulmonary Mesothelioma-Associated Molecule that Regulates Metabolic Stress (2012)
  • Author(s): Yanagisawa K et al
  • Organizer: ECHANISMS & MODELS OF CANCER
  • Place of Presentation: Cold Spring Harbor Laboratory, USA
  • Year and Date: 2012-08-18
• [Presentation] Proteomic identification of potential biomarkers of malignant pulmonary mesothelioma (2012)
  • Author(s): Yanagisawa K., et al.
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Invited
• [Presentation] Quantitative Proteomic Profiling Identifies CKAP4 as a MalignantPulmonary Mesothelioma-Associated Molecule that Regulates Metabolic Stress (2012)
  • Author(s): Yanagisawa K., et al.
  • Organizer: MECHANISMS & MODELS OF CANCER
  • Place of Presentation: Cold Spring Harbor Laboratory, USA
• [Presentation] Proteomic analyses for biomarker discovery in human pancreatic cancer (2011)
  • Author(s): Kawahara T. and Yanagisawa K et al.
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場、名古屋
  • Year and Date: 2011-10-03
• [Presentation] CKAP4, a novel malignant pulmonary mesothelioma-related gene regulates eIF2αand a cellular stress-response (2011)
  • Author(s): Yanagisawa K et al
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場、名古屋
  • Year and Date: 2011-10-03
• [Presentation] CKAP4, a novel malignant pulmonary mesothelioma-related gene, regulates eIF2α and a cellular stress-response (2011)
  • Author(s): Yanagisawa K., et al
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-03
• [Presentation] Proteomic analyses for biomarker discovery in human pancreatic cancer (2011)
  • Author(s): Kawahara T., et al
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-03
• [Presentation] 新規肺癌転移関連遺伝子CIMの機能解析 (2010)
  • Author(s): 柳澤聖
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-23
• [Presentation] Proteomic analyses for biomarker discovery in human malignant pleural mesothelioma (2010)
  • Author(s): Kato S and Yanagisawa K et al
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場、大阪
  • Year and Date: 2010-09-22
• [Presentation] CIM, novel lung cancer metastasis gene, regulates multiple cellular stress-response pathways (2010)
  • Author(s): Yanagisawa K et al
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場、大阪
  • Year and Date: 2010-09-22
• [Remarks] 特記事項なし
• [Patent(Industrial Property Rights)] 胸膜中皮腫患者の早期発見のための分子マーカーの組合せとその発現解析方法 (2013)
  • Inventor(s): 柳澤 聖、高橋 隆、横井香平、長谷川好規、小野健一郎、八木香澄、上原理恵、藤井慶宏、岸 義朗、竹内俊幸
  • Industrial Property Rights Holder: 柳澤 聖、高橋 隆、横井香平、長谷川好規、小野健一郎、八木香澄、上原理恵、藤井慶宏、岸 義朗、竹内俊幸
  • Filing Date: 2013-02-28
• [Patent(Industrial Property Rights)] 胸膜中皮腫患者の早期発見のための分子マーカーの組合せとその発現解析方法 (2013)
  • Inventor(s): 柳澤 聖 他９名
  • Industrial Property Rights Holder: 柳澤 聖 他９名
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-02-28
• [Patent(Industrial Property Rights)] 胸膜中皮腫患者の早期発見のための分子マーカー及びその発現解析方法 (2011)
  • Inventor(s): 柳澤 聖、高橋 隆、横井香平、長谷川好規、小野健一郎、八木香澄、増田 瞳、竹内俊幸柳澤 聖、高橋 隆、横井香平、長谷川好規、小野健一郎、八木香澄、増田 瞳、竹内俊幸
  • Industrial Property Rights Holder: 柳澤 聖、高橋 隆、横井香平、長谷川好規、小野健一郎、八木香澄、増田 瞳、竹内俊幸
  • Filing Date: 2011-09-15
• [Patent(Industrial Property Rights)] 胸膜中皮腫患者の早期発見のための血液分子マーカー及びその発現解析方法 (2011)
  • Inventor(s): 柳澤聖, 他8名
  • Industrial Property Rights Holder: 国立大学法人名古屋大学、株式会社医学生物学研究所、株式会社Oncomics
  • Industrial Property Number: 2011-202463
  • Filing Date: 2011-09-15