INVOLVMENT OF TH17 CELLS IN TUMOR ANGIOGENESIS INDUCED BY INTERACION BETWEEN NON-SMALL CELL LUNG CANCER CELLS AND INTERSTITIAL STROMAL CELLS

• Project/Area Number: 22590869
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Josai University
• Principal Investigator: NUMASAKI Muneo 城西大学, 薬学部, 教授 (50344677)
• Co-Investigator(Kenkyū-buntansha): HAGIWARA Kouiti 埼玉医科大学, 医学部, 教授 (00240705)
• Co-Investigator(Renkei-kenkyūsha): TAGAWA Masatoshi (20171572)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: サイトカイン / 肺癌 / Th17 細胞 / 血管新生 / 血管新生因子 / Th17

Research Abstract

Th17 cells were isolated and culture supernatants were collected. Culture supernatants of Th17 cells were found to have the activity to induce neovascular formation, and this activity was inhibited, in part, by an anti-IL-17 antibody.

Research Products

• [Journal Article] A distinct regulatory role of Th17 cytokines IL-17A and IL-17F in chemokine secretion from lung microvascular endothelial cells (2012)
  • Author(s): Fujie H、 Niu K、 Ohba M、Tomioka Y、Kitazawa H、Nagashima K、Ohrui T 、 Numasaki M
  • Journal Title: Inflammation Volume: 35 Issue: 3 Pages: 1119-1131
  • DOI: 10.1007/s10753-011-9419-0
  • Peer Reviewed
• [Journal Article] A Distinct Regulatory Role of Th17 Cytokines IL-17A and IL-17F in Chemokine Secretion from Lung Microvascular Endothelial Cells (2012)
  • Author(s): Fujie H, Niu K, Ohba M, Tomioka Y, Kitazawa H, Nagashima K, Ohrui T, Numasaki M
  • Journal Title: Inflammation Volume: (in press)
  • Peer Reviewed
• [Journal Article] Adenoviruses-mediated transduction of human esophageal carcinoma cells with the interferon-lambda genes produced anti-tumor effects (2011)
  • Author(s): Li, Q., Kawamura, K., Okamoto, S., Fujie, H., Numasaki, M., Namba, M., Nagata, M., Shimada, H., Kobayashi, H. and Tagawa, M.
  • Journal Title: Br. J. Cancer Volume: 105 Issue: 9 Pages: 1302-1312
  • DOI: 10.1038/bjc.2011.379
  • Peer Reviewed
• [Journal Article] Antitumor activity of type III interferon alone or in combination with type I interferon against human non-small cell lung cancer (2011)
  • Author(s): Fujie H、 Tanaka T、Tagawa M、 Kaijun N、 Watanabe M、 Suzuki T、 Nakayama K、 Numasaki M
  • Journal Title: Cancer Sci Volume: 102 Issue: 11 Pages: 1977-1990
  • DOI: 10.1111/j.1349-7006.2011.02079.x
  • NAID: 120005518441
  • Peer Reviewed
• [Journal Article] Toll-like receptor-2 activating bifidobacteria strains differentially regulate inflammatory cytokines in porcine intestinal epithelial cell culture system : finding new anti-inflammatory immunobiotics (2011)
  • Author(s): Fujie, H., H.Aso, Y.Suda, T.Saito, H.Kitazawa, et al
  • Journal Title: FEMS Immunol.Med.Microbiol. Volume: 63 Issue: 1 Pages: 129-139
  • DOI: 10.1111/j.1574-695x.2011.00837.x
  • Peer Reviewed
• [Journal Article] Molecular cloning, tissue expression, and subcellular localization of porcine peptidoglycan recognition proteins 3 and 4 (2011)
  • Author(s): Ueda, W., H.Aso, Y.Kawai, T.Saito, H.Kitazawa, et al
  • Journal Title: Vet.Immunol.Immunopathol. Volume: 143 Issue: 1-2 Pages: 148-154
  • DOI: 10.1016/j.vetimm.2011.05.026
  • Peer Reviewed
• [Journal Article] Adenoviruses-mediated transduction of human oesophageal carcinoma cells with the interferon-1 genes produced anti-tumour effects (2011)
  • Author(s): Li Q, Kawamura K, Okamoto S, Fujie H, Numasaki M, Namba M, Nagata M, Shimada H, Kobayashi H, Tagawa M
  • Journal Title: British Journal of Cancer Volume: 105 Pages: 1302-1312
  • Peer Reviewed
• [Journal Article] Antitumor activity of type III interferon alone or in combination with type I interferon against human non-small cell lung cancer (2011)
  • Author(s): Fujie H, Tanaka T, Tagawa M, Kaijun N, Watanabe M, Suzuki T, Nakayama K, Numasaki M
  • Journal Title: Cancer Science Volume: 102 Pages: 1977-1990
  • NAID: 120005518441
  • Peer Reviewed
• [Journal Article] Toll-like receptor-2-activating bifidobacteria strains differentially regulate inflammatory cytokines in the porcine intestinal epithelial cell culture system : finding new anti-inflammatory immunobiotics (2011)
  • Author(s): Fujie H, Villena J, Tohno M, Morie K, Shimazu T, Aso H, Suda Y, Shimosato T, Iwabuchi N, Xiao JZ, Yaeshima T, Iwatsuki K, Saito T, Numasaki M, Kitazawa H
  • Journal Title: FEMS immunology and medical microbiology Volume: 63 Pages: 129-139
  • Peer Reviewed
• [Journal Article] Molecular cloning, tissue expression, and subcellular localization of porcine peptidoglycan recognition proteins 3 and 4 (2011)
  • Author(s): Ueda W, Tohno M, Shimazu T, Fujie H, Aso H, Kawai Y, Numasaki M, Saito T, Kitazawa H
  • Journal Title: Veterinary Immunology and Immunopathology Volume: 43 Pages: 148-154
  • Peer Reviewed
• [Journal Article] Immunobiotic Lactobacillus jensenii elicit anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway. (2011)
  • Author(s): Tomoyuki Shimazu, Julio Villena, Masanori Tohno, Hitomi Fujie, Takeshi Shimosato, Hisashi Aso, Yoshihito Suda, Yasushi Kawai, Tadao Saito, Seiya Makino, Shuji Ikegami, Hiroyuki Itoh, Muneo Numasaki, Haruki Kitazawa
  • Journal Title: Infection and Immunity Volume: (In press)
  • Peer Reviewed
• [Journal Article] A Distinct Regulatory Role of the Th17 Cytokines IL-17A and IL-17F in Chemokine Secretion from Lung Microvascular Endothelial Cells. (2011)
  • Author(s): Hitomi Fujie, Saori Ishiyama, Masatoshi Tagawa, Niu Kaijun, Haruki Kitazawa, Yoshihisa Tomioka, Muneo Numasaki.
  • Journal Title: Inflammation Volume: (In press)
  • Peer Reviewed
• [Journal Article] Type III interferon alone or in combination with type I interferon inhibits the growth of human non-small cell lung cancer in vitro and in vivo. (2011)
  • Author(s): Hitomi Fujie, Toshiaki Tanaka, Saori Ishiyama, Masatoshi Tagawa, Katsutoshi Nakayama, Takashi Suzuki, Muneo Numasaki.
  • Journal Title: Cancer Science Volume: (In press)
  • Peer Reviewed
• [Journal Article] Interferon-lambda induces G1 phase arrest or apoptosis in oesophageal carcinoma cells and produces anti-tumour effects in combination with anti-cancer agents (2010)
  • Author(s): Li Q、 Kawamura K、 Ma G、 Iwata F、 Numasaki M、 Suzuki N、 Shimada H、Tagawa M
  • Journal Title: Eur. J. Cancer Volume: 46 Issue: 1 Pages: 180-190
  • DOI: 10.1016/j.ejca.2009.10.002
  • Peer Reviewed
• [Presentation] Toll-like receptor-2 activating bifidobacteria strains regulate inflammatory responces in porcine intestinal epithelial cells (2011)
  • Author(s): Fujie H、Villena J、Tohno M、Morie K、Shimazu T、Aso H、Suda Y、Shimosato T、Iwabuchi N、Xiao JZ、Yaeshima T、Iwatsuki K、Numasaki M、Kawai Y、Saito T、Kitazawa H
  • Organizer: FEMS Congress of European Microbiologists
  • Place of Presentation: スイス
  • Year and Date: 2011-06-27
• [Presentation] Th17サイトカインと肺癌、シンポジウム炎症と肺癌-発症、進展における共通のメカニズムを探る- (2011)
  • Author(s): 沼崎宗夫
  • Organizer: 第51回日本呼吸器学会学術講演会
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2011-04-22
• [Presentation] IL-17 cytokine family IL-17A、IL-17A/F及びIL-17Fによる気道上皮細胞のケモカイン産生の制御 (2011)
  • Author(s): 野口愛、沼崎宗夫
  • Organizer: 第51回日本呼吸器学会学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2011-04-22
• [Presentation] IL-17は、血管内皮細胞上のフラクタルカインが誘導するNK cellの活性化により腫瘍の肺転移を抑制する (2011)
  • Author(s): 成井ももこ、沼崎宗夫
  • Organizer: 第51回日本呼吸器学会学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2011-04-22
• [Presentation] Toll-like receptor-2 activating bifidobacteria strains regulate inflammatory responces in porcine intestinal epithelial cells (2011)
  • Author(s): Fujie H、Villena J、Tohno M、Morie K、Shimazu T、Aso H、Suda Y、Shimosato T、Iwabuchi N、Xiao JZ、Yaeshima T、Iwatsuki K、Numasaki M、Kawai Y、Saito T、Kitazawa H
  • Organizer: 4th FEMS Congress of European Microbiologists
  • Place of Presentation: スイス
• [Presentation] Th17 サイトカインと肺癌、シンポジウム 炎症と肺癌-発症、進展における共通のメカニズムを探る- (2011)
  • Author(s): 沼崎宗夫
  • Organizer: 第51回日本呼吸器学会学術講演会
  • Place of Presentation: 東京
• [Presentation] IL-17 cytokine family IL-17A、IL-17A/F 及び IL-17F による気道上皮細胞のケモカイン産生の制御 (2011)
  • Author(s): 野口 愛、 沼崎宗夫
  • Organizer: 第51回日本呼吸器学会学術講演会
  • Place of Presentation: 東京
• [Presentation] IL-17 は、血管内皮細胞上のフラクタルカインが誘導するNK cell の活性化により腫瘍の肺転移を抑制する (2011)
  • Author(s): 成井ももこ、沼崎宗夫
  • Organizer: 第51回日本呼吸器学会学術講演会
  • Place of Presentation: 東京
• [Presentation] インターフェロンラムダ発現型アデノウイルスによる食道がんに対する抗腫瘍効果 (2010)
  • Author(s): 田川雅敏, 川村希代子, 岡本慎也, 楊珊, 深町利彦, 小林弘, 藤江瞳, 沼崎宗夫, 島田英昭
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪府大阪市・大阪国際会議場
  • Year and Date: 2010-09-23
• [Presentation] IL-17は血管内皮細胞のフラクタルカインの発現を増強することにより腫瘍の肺転移を抑制する (2010)
  • Author(s): 藤江瞳, 田川雅敏, 沼崎宗夫
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪府大阪市・大阪国際会議場
  • Year and Date: 2010-09-22
• [Presentation] Adenoviruses-mediated expression and cell-mediated delivery of interferon-lambda achieve anti-tumor effects in vivo. (2010)
  • Author(s): Msatoshi Tagawa, Quanhai Li, Kiyoko Kawamura, Shinya Okamoto, Shan Yang, Hitomi Fujie, Muneo Numasaki, Hideaki Shimada, Hiroshi Kobayashi
  • Organizer: 第16回日本遺伝子治療学会
  • Place of Presentation: 栃木県宇都宮市・栃木県総合文化センター
  • Year and Date: 2010-07-02
• [Presentation] Adenoviruses-mediated expression and cell-mediated delivery of interferon-lambda produced anti-tumor effects to human esophageal carcinoma in vivo. (2010)
  • Author(s): Masatoshi Tagawa, Quanhai Li, Kiyoko Kawamura, Shinya Okamoto, Shan Yang, Hiroshi Kobayashi, Muneo Numasaki, Yuji Tada, Kenzo Hiroshima, Hideaki Shimada.
  • Organizer: ASGCT 2010 Clinical Trials Training Course and 13th Annual Meeting
  • Place of Presentation: Mariott Wardman Park Hotel, Washington, DC, USA
  • Year and Date: 2010-05-22
• [Presentation] The additive cytostatic effect of Type I and Type III IFNs against human NSCLC. (2010)
  • Author(s): Hitomi Fujie, Muneo Numasaki, Hidenori Takahashi
  • Organizer: 第50回日本呼吸器学会学術講演会
  • Place of Presentation: 京都府京都市・国立京都国際会館
  • Year and Date: 2010-04-23
• [Presentation] IL-17 は血管内皮細胞のフラクタルカインの発現を増強することにより腫瘍の肺転移を抑制する (2010)
  • Author(s): 藤江瞳、 田川雅敏、 沼崎宗夫
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
• [Presentation] インターフェロンラムダ発現型アデノウイルスによる食道がんに対する抗腫瘍効果 (2010)
  • Author(s): 田川雅敏、川村希代子、岡本慎也、楊珊、深町利彦、小林弘、藤江瞳、沼崎宗夫、島田英昭
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
• [Presentation] Adenoviruses-mediated expression and cell-mediated delivery of interferon-lambda produced anti-tumor effects to human esophageal carcinoma in vivo (2010)
  • Author(s): Msatoshi Tagawa 、 Quanhai Li 、 Kiyoko Kawamura 、 Shinya Okamoto 、 Shan Yang 、Hiroshi Kobayashi 、Muneo Numasaki 、Yuji Tada、 Kenzo Hiroshima、 and Hideaki Shimada
  • Organizer: ASGCT 2010 Clinical Trials Training Course and 13th Annual Meeting
  • Place of Presentation: アメリカ
• [Presentation] Adenoviruses-mediated expression and cell-mediated delivery of interferon-lambda achieve anti-tumor effects in vivo (2010)
  • Author(s): Msatoshi Tagawa 、 Quanhai Li 、 Kiyoko Kawamura、 Shinya Okamoto、 Shan Yang、 Hitomi Fujie 、Muneo Numasaki 、Hideaki Shimada 、and Hiroshi Kobayashi
  • Organizer: 第16回日本遺伝子治療学会
  • Place of Presentation: 栃木
• [Presentation] The additive cytostatic effect of Type I and Type IIIIFNs against human NSCLC (2010)
  • Author(s): Hitomi Fujie 、 Muneo Numasaki 、 and Hidenori Takahash
  • Organizer: 第50回日本呼吸器学会学術講演会
  • Place of Presentation: 京都
• [Book] Advancements in Tumor Immunotherapy and Cancer Vaccines (2012)
  • Author(s): Hitomi Fujie, Muneo Numasaki
  • Publisher: InTech
• [Book] Type III Interferons IL-28 and IL-29: Novel Interferon Family Members with Therapeutic Potential in Cancer Therapy. In Advancements in Tumor Immunotherapy and Cancer Vaccines (2011)
  • Author(s): Fujie H 、Numasaki M
  • Publisher: In Advancements in Tumor Immunotherapy and Cancer Vaccines
• [Remarks]
  • URL: http://www.josai.ac.jp/~facpharm/pharma4/yk/department_of_nutritional_physiology.html