Development of early diagnosis and disease stage evaluation method using ribosomal RNA gene in respiratory bacterial infectious diseases
| Project/Area Number |
22590875
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
HOSHINO Yoshihiko 国立感染症研究所, ハンセン病研究センター 感染制御部, 室長 (20569694)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 呼吸器感染症 / 抗酸菌 / 全ゲノム解析 / 新興・再興感染症 / 定量的PCR / T-RFLP / リボゾームRNA / 16SrRNA / 16S rRNA |
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| Research Abstract |
We developed rapid diagnosis and disease stage evaluation method for pulmonary bacterial infection. Especially we focused pulmonary mycobacterial infection in this study. Principally, 16S ribosomal RNA (rRNA) gene was targeted and other genes such as internal transcribed spacer (ITS), heat shock protein 65 (hsp65), and RNA polymerase beta subunit (rpoB) were adjunctively utilized. We noticed the similarity of 16S rRNA genes among mycobacteria species and the necessity of combination of other genes.
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Report
(4 results)
Research Products
(65 results)
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[Journal Article] Bacteremia due to Mycobacterium massiliense in a patient with chronic myelogenous leukemia:2012
Author(s)
Hamamoto T, Yuki A, Naoi K, Kawakami S, Banba Y, Yamamura T, Hikota R, Watanabe J, Kimura F, Nakanaga K, Hoshino Y, Ishii N, Shimazaki H, Nakanishi K, Tamai S.
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Journal Title
Diagnostic Microbiology and Infectious Disease
Volume: 74(2)
Pages: 183-5
Related Report
Peer Reviewed
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