| Project/Area Number |
22590878
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TAKASE Osamu 東京大学, 医学部附属病院, 特任助教 (60265684)
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| Co-Investigator(Kenkyū-buntansha) |
HISHIKAWA Keiichi 東京大学, 医学部附属病院, 特任准教授 (50255460)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJITA Toshiro 東京大学, 先端科学技術研究センター, 教授 (10114125)
ABURATANI Hiroyuki 東京大学, 先端科学技術研究センター, 教授 (10202657)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 腎臓由来iPS細胞 / NF-kappaB / siRNA Knockdown / HDAC阻害剤 / エピジェネティク・メモリー / 腎上皮由来iPS細胞 / 腎臓上皮細胞由来iPS細胞 / 線維芽細胞由来iPS細胞 / TSA処置(HDAC阻害剤) / 皮膚線維芽細胞由来iPS細胞 / siRNA Knockdown処置 / 特異的分化 |
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| Research Abstract |
We have investigated about the differentiation toward to kidney using human Renal-iPS (hR-iPS) cells regulated by NF-kB as compared with human Fibroblastic-iPS (hF-iPS) cells. Both iPS cells have shown the activity of NF-kB. As NF-kB activity was knockdown by NF-kB p65 siRNA, the expression of undifferentiated makers decreased, conversely the kidney-associated-differentiated makers increased, especially stronger in hR-iPS than hF-iPS cells. Thus we have suggested that the augmentation of NF-B signaling maintains the undifferentiated state of human iPS, and hR-iPS cells are easy of differentiation toward to kidney. We have reported a part of outcome at the annual meeting of the Japanese Society of Nephrology, Japanese Society of Regenerative Medicine, and to the Journal of ‘PLoS One’.
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