High-throughput multiplex quantitation of urinary protein biomarkers using a triple quadrupole mass spectrometer

• Project/Area Number: 22590881
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Niigata University
• Principal Investigator: YOSHIDA Yutaka 新潟大学, 医歯学系, 講師 (40182795)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Tadashi 新潟大学, 医歯学系, 教授 (30092737)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 腎臓学 / 尿バイオマーカー / 尿プロテオーム / 分析化学 / 質量分析 / 多項目同時定量 / AKIバイオマーカー / 尿バイオマーカーパネル化

Research Abstract

A Proteomic analysis of normal urine proteome and seeking of urinary proteins originated from the glomerulus, we found biomarker candidates of tubular injury (NGAL, L-FABP, KIM-1, Megalin) and glomerulus injury (Podocalyxin, SHPS-1, Crumbs-like protein 2). We now constructed SRM transition conditions for multiplexed assay of these biomarkers using TripleQ-mass spectrometer.

Research Products

• [Journal Article] Profiling and annotation of human kidney glomerulus proteome (2013)
  • Author(s): Cui Z, Yoshida Y, Xu B, Zhang Y, Nameta M, Magdeldin S, Makiguchi T, Ikoma T, Fujinaka H, Yaoita E, Yamamoto T
  • Journal Title: Proteome Sci Volume: 11 Issue: 1 Pages: 13-13
  • DOI: 10.1186/1477-5956-11-13
  • Peer Reviewed
• [Journal Article] Profiling of kidney vascular endothelial cell plasma membrane proteins by liquid chromatography-tandem mass spectrometry (2012)
  • Author(s): Liu Z, Xu B, Nameta M, Zhang Y, Magdeldin S, Yoshida Y, Yamamoto K, Fujinaka H, Yaoita E, Tasaki M, Nakagawa Y, Saito K, Takahashi K, Yamamoto T
  • Journal Title: Clin Exp Nephrol Volume: 掲載予定 Issue: 3 Pages: 327-337
  • DOI: 10.1007/s10157-012-0708-1
  • NAID: 10031184238
  • Peer Reviewed
• [Journal Article] Murine colon proteome and characterization of the protein pathways. (2012)
  • Author(s): Magdeldin S
  • Journal Title: BioData Min. Volume: 5 Issue: 1 Pages: 11-11
  • DOI: 10.1186/1756-0381-5-11
  • Peer Reviewed
• [Journal Article] Proteomic approach to human kidney glomerulus prepared by laser microdissection from frozen biopsy specimens: exploration of proteome after removal of blood-derived proteins (2012)
  • Author(s): Yoshida Y, Nameta M, Kuwano M, Zhang Y, Bo X, Magdeldin S, Cui Z, Fujinaka H, Yaoita E, Tomonaga T, Yamamoto T
  • Journal Title: Proteomics Clin Appl Volume: 6 Issue: 7-8 Pages: 412-417
  • DOI: 10.1002/prca.201200016
  • Peer Reviewed
• [Journal Article] Comparison of human glomerulus proteomic profiles obtained from low quantities of samples by different mass spectrometry with the comprehe nsive database (2011)
  • Author(s): Ying Zhang
  • Journal Title: Proteome Science Volume: VOL.9 Issue: 1 Pages: 47-57
  • DOI: 10.1186/1477-5956-9-47
  • Peer Reviewed
• [Journal Article] Usage of electrostatic eliminator reduces human keratin contamination significantly in gel-based proteomics analysis (2011)
  • Author(s): 許波
  • Journal Title: Journal of Proteomics Volume: VOL.74 Issue: 7 Pages: 1022-1029
  • DOI: 10.1016/j.jprot.2011.03.001
  • Peer Reviewed
• [Journal Article] Novel expression of claudin-5 in glomerular podocytes (2011)
  • Author(s): Koda R, Zhao L, Yaoita E, Yoshida Y, Tsukita S, Tamura A, Nameta M, Zhang Y, Fujinaka H, Magdeldin S, Xu B, Narita I, Yamamoto T
  • Journal Title: Cell Tissue Res Volume: 343(3) Issue: 3 Pages: 637-48
  • DOI: 10.1007/s00441-010-1117-y
  • Peer Reviewed
• [Journal Article] Differential proteomic shotgun analysis elucidates involvement of water channel aquaporin 8 in presence of α-amylase in the colon (2010)
  • Author(s): Magdeldin S, Li H, Yoshida Y, Satokata I, Maeda Y, Yokoyama M, Enany S, Zhang Y, Xu B, Fujinaka H, Yaoita E, Yamamoto T
  • Journal Title: J Proteome Res Volume: 9 Issue: 12 Pages: 6635-46
  • DOI: 10.1021/pr100789v
  • Peer Reviewed
• [Journal Article] Glomerular proteins related to slit diaphragm and matrix adhesion inthe foot processes are highly tyrosine phosphorylated in the normal ratrat kidney. (2010)
  • Author(s): Y.Zhang
  • Journal Title: Nephrol.Dial.Transplant. Volume: 25 Pages: 1785-1795
  • Peer Reviewed
• [Journal Article] Functional proteomic analysis of experimental autoimmune myocarditis-Induced chronic heart failure in the rat (2010)
  • Author(s): Y.Sanzen
  • Journal Title: Biol.Pharm.Bull. Volume: 33 Pages: 477-486
  • Peer Reviewed
• [Journal Article] Comparison of two dimensional electrophoresis of mouse colonProteomes before and after knocking out Aquaporin 8 (2010)
  • Author(s): S.Magdeldin
  • Journal Title: J Proteomics Volume: 10 Pages: 2031-2010
  • Peer Reviewed
• [Journal Article] Differential proteomic shotgun analysis elucidates involvement of water Channel aquaporin 8 in presence of alpha-amylase in the colon. (2010)
  • Author(s): S.Magdeldin
  • Journal Title: J Proteome Res. Volume: 6 Pages: 6635-6646
  • Peer Reviewed
• [Presentation] Comparative proteomic analysis of human glomerulus prepared by laser microdissection from frozen and FFPE kidney tissues (2012)
  • Author(s): Yutaka Yoshida
  • Organizer: 8th Management Committee meeting of the COST action EuroKUP (BM 0702)
  • Place of Presentation: Athens, Aegeon Hotel(招待講演)
  • Year and Date: 2012-03-30
• [Presentation] Increased identification of low-abundance, insoluble proteins of glomerulus laser-microdissected from frozen tissue sections exposed to repeated PBS washing (2012)
  • Author(s): Yoshida Y et al
  • Organizer: 第11回国際ヒトプロテオーム機構学術集会
  • Place of Presentation: ボストン(アメリカ合衆国)
• [Presentation] HKUPP: Human Kidney andUrine Proteome Project (2012)
  • Author(s): 吉田 豊
  • Organizer: 日本プロテオーム学会2012年大会(第10回日本ヒト プロテオーム機構)
  • Place of Presentation: 東京
• [Presentation] 凍結腎生検試料からLaser microdissectionにより調製した糸球体のプロテオーム解析:血液由来タンパク質の除去 (2012)
  • Author(s): 吉田豊他
  • Organizer: 日本プロテオーム学会2012年大会(第10回日本ヒトプロテオーム機構)
  • Place of Presentation: 東京
• [Presentation] Increased identification of low-abundance, insoluble proteins of glomerulus laser-microdissected from frozen tissue sections exposed to repeated PBS washing. (2012)
  • Author(s): Yutaka Yoshida
  • Organizer: 第11回国際ヒトプロテオーム機構学術集会
  • Place of Presentation: ボストン (アメリカ合衆国）
• [Presentation] HKUPP: Human Kidney and Urine Proteome Project. (2012)
  • Author(s): 吉田 豊
  • Organizer: 日本プロテオーム学会2012年大会（第10回日本ヒトプロテオーム機構）
  • Place of Presentation: 東京
  • Invited
• [Presentation] Extensive profiling of human urine proteome : construction of SRM transitions for quantification of urinary biomarkers for acute and chronic kidney diseases (2011)
  • Author(s): Yutaka Yoshida
  • Organizer: HUPO 2011 10th World Congress
  • Place of Presentation: Geneva, Palexepo Conference hall
  • Year and Date: 2011-09-06
• [Presentation] Verification and standardization of comparative proteomic analysis of human glomeruli prepared by laser microdissection from frozen block (2011)
  • Author(s): Yutaka Yoshida
  • Organizer: 日本プロテオーム学会2011年会サテライトシンポジウム
  • Place of Presentation: 新潟朱鷺メッセ(招待講演)
  • Year and Date: 2011-07-30
• [Presentation] 健常人尿プロテオームの特徴:AKIバイオマーカーの多項目同時測定の基礎的検討 (2011)
  • Author(s): 吉田豊
  • Organizer: 第54回日本腎臓学会
  • Place of Presentation: 横浜パシフィコ横浜
  • Year and Date: 2011-06-15
• [Presentation] Extensive profiling of human urine proteome: construction of SRM transitions for quantification of urinary biomarkers for acute and chronic kidney diseases (2011)
  • Author(s): Yoshida Y et al
  • Organizer: 第10回国際ヒトプロテオーム機構学術集会
  • Place of Presentation: ジュネーブ(スイス連邦)
• [Presentation] 正常ヒト尿プロテオームの特徴:AKIバイオマーカーの多項目同時測定の基礎的検討 (2011)
  • Author(s): 吉田豊他
  • Organizer: 日本プロテオーム学会2011年大会(第9回日本ヒトプロテオーム機構)
  • Place of Presentation: 新潟
• [Presentation] On-site proteomics study on laser microdissected rat renal cortex and human glomerulus tissues (2010)
  • Author(s): Xu Bo
  • Organizer: 国際プロテオーム機構第9回国際大会
  • Place of Presentation: シドニー
  • Year and Date: 2010-09-20
• [Presentation] Verification and standardization of comparative proteomic analysis of human glomerululi prepared by laser microdissection from frozen block (2010)
  • Author(s): Yutaka Yoshida
  • Organizer: 国際プロテオーム機構第9回国際大会
  • Place of Presentation: シドニー
  • Year and Date: 2010-09-20
• [Presentation] High confidence proteomic profile of minute amount of human kidney glomeruli (2010)
  • Author(s): Ying Zhang
  • Organizer: 国際プロテオーム機構第9回国際大会
  • Place of Presentation: シドニー
  • Year and Date: 2010-09-20
• [Presentation] In-depth analysis of aquaporin 8 knockout mice colon using tandem mass spectrometry (2010)
  • Author(s): Sameh Magdeldin
  • Organizer: 国際プロテオーム機構第9回国際大会
  • Place of Presentation: シドニー
  • Year and Date: 2010-09-20
• [Presentation] Exploring resistant determinants of methicillin in Staphylococcus Aureuswith comparative proteomics approach. (2010)
  • Author(s): Shymaa Enany
  • Organizer: 国際プロテオーム機構第9回国際大会
  • Place of Presentation: シドニー
  • Year and Date: 2010-09-20
• [Presentation] High confidence proteomic profile of minute amount of human kidney glomeruli (2010)
  • Author(s): 張螢
  • Organizer: 日本プロテオーム学会2010年大会
  • Place of Presentation: 舞浜
  • Year and Date: 2010-07-26
• [Presentation] Laser microdissectionを用いた腎生検試料の糸球体プロテオーム解析 (2010)
  • Author(s): 吉田豊
  • Organizer: 日本プロテオーム学会2010年大会
  • Place of Presentation: 舞浜
  • Year and Date: 2010-07-26
• [Presentation] In-depth proteomic analysis of secretome of European community-Acquired methicillin-resistant Staphylococcus aureus (2010)
  • Author(s): Enany Shymaa
  • Organizer: 日本プロテオーム学会2010年大会
  • Place of Presentation: 舞浜
  • Year and Date: 2010-07-26
• [Presentation] Laser microdissectionを用いたホルマリン固定標本のon-siteプロテオーム解析 (2010)
  • Author(s): 許波
  • Organizer: 日本プロテオーム学会2010年大会
  • Place of Presentation: 舞浜
  • Year and Date: 2010-07-26
• [Presentation] Proteomic profiling of aquaporin 8 knockout mice reveals its involvement in alpha amylase regulation (2010)
  • Author(s): Magdaldin Sameh
  • Organizer: 日本プロテオーム学会2010年大会
  • Place of Presentation: 舞浜
  • Year and Date: 2010-07-26
• [Presentation] High confidence proteomic profile of minute amount of human kidney glomeruli (2010)
  • Author(s): 張螢
  • Organizer: 第53回日本腎臓学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-06-18
• [Presentation] ホルマリン固定標本とLaser microdissectionを用いたon-siteプロテオーム解析 (2010)
  • Author(s): 許波
  • Organizer: 第53回日本腎臓学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-06-18
• [Presentation] Laser microdissectionとLC-MSを用いた腎生検試料の糸球体プロテオーム解析 (2010)
  • Author(s): 吉田豊
  • Organizer: 第53回日本腎臓学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-06-17
• [Presentation] Proteomic analysis elucidates involvement of water channel aquaporin 8 in alpha amylase regulation (2010)
  • Author(s): Magdaldin Sameh
  • Organizer: 第53回日本腎臓学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-06-17
• [Presentation] 尿中インスリン様成長因子結合蛋白(IGFBP)の意義 (2010)
  • Author(s): 藤中秀彦
  • Organizer: 第53回日本腎臓学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-06-16
• [Presentation] Verification and standardization of comparative proteome analysis of human glomeruli prepared by laser microdissectionfrom frozen block (2010)
  • Author(s): Yoshida Y
  • Organizer: 第9回国際ヒトプロテオーム機構学術集会
  • Place of Presentation: シドニー(オーストラリア連邦)
• [Presentation] Laser microdissectionとLC-MSを用いた腎生検試料の糸球体プロテオーム解析 (2010)
  • Author(s): 吉田豊他
  • Organizer: 第53回日本腎臓学会学術総会
  • Place of Presentation: 神戸
• [Remarks]
  • URL: http://hkupp.kir.jp
• [Remarks] Human Kidney and Urine Proteome Project
  • URL: http://www.hkupp.org/
• [Remarks]
  • URL: http://hkupp.kir.jp/DB.html
• [Remarks]
  • URL: http://www.hkupp.org/