Therapeutic approaches of AKI by adipose-derived mesenchymal cells, and urinary biomarker search in AKI

• Project/Area Number: 22590883
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Kanazawa University
• Principal Investigator: FURUICHI Kengo 金沢大学, 大学病院, 准教授 (50432125)
• Co-Investigator(Kenkyū-buntansha): WADA Takashi 金沢大学, 医学系, 教授 (40334784)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: バイオマーカー / 虚血腎障害 / 脂肪由来間葉系細胞 / 質量分析計 / IP-10 / 再生 / サイトカイン / ケモカイン

Research Abstract

everal urinary biomarker candidates of acute kidney injury were detected by mass spectrometry. We also detected several urinary biomarker candidates of acute kidney injury by multiple cytokine/chemokine detection methods. In this study, we explored the effects of adipose-derived mesenchymal cells on a mouse model of AKI. The effects of adipose-derived mesenchymal cells were evaluated in AKI mouse model. A lthough, administered adipose-derived mesenchymal cells were tend to home into lung, repeated administration of adipose-derived mesenchymal cells reduced acute tubular necrosis and interstitial macrophage infiltration.

Research Products

• [Journal Article] Fas Ligand Has a Greater Impact than TNF-α on Apoptosis and Inflammation in Ischemic Acute Kidney Injury (2012)
  • Author(s): Furuichi K, Kokubo S, Hara A, Imamura R, Wang Q, Kitajima S, Toyama T, Okumura T, Matsushima K, Suda T, Mukaida N, Kaneko S, Wada T
  • Journal Title: Nephron Extra Volume: 2 Issue: 1 Pages: 27-38
  • DOI: 10.1159/000335533
  • Peer Reviewed
• [Journal Article] Effects of adipose-derived mesenchymal cells on ischemia-reperfusion injury in kidney. (2012)
  • Author(s): Furuichi K, Shintani H, Sakai Y, Ochiya T, Matsushima K, Kaneko S, Wada T
  • Journal Title: Clin Exp Nephrol Volume: 16 Issue: 5 Pages: 679-89
  • DOI: 10.1007/s10157-012-0614-6
  • NAID: 10031122254
  • Peer Reviewed
• [Journal Article] Glycoprotein hyposialylation gives rise to a nephrotic-like syndrome that is prevented by sialic acid administration in GNE V572L point-mutant mice (2012)
  • Author(s): Ito, M., Sugihara, K., Asaka, T., Toyama, T., Yo sh ih ar a, T., Furuichi, K., Wada, T. and Asano, M.
  • Journal Title: PLoS ONE Volume: 7 Issue: 1 Pages: 1-13
  • DOI: 10.1371/journal.pone.0029873
  • Peer Reviewed
• [Journal Article] Activation of p38 mitogen-activated protein kinase promotes peritoneal fibrosis by regulating fibrocytes (2012)
  • Author(s): Kokubo S, Sakai N, Furuichi K, Toyama T, Kitajima S, Okumura T, Matsushima K, Kaneko S, Wada T
  • Journal Title: Perit Dial Int Volume: 32 Issue: 1 Pages: 10-9
  • DOI: 10.3747/pdi.2010.00200
  • Peer Reviewed
• [Journal Article] AKIとCKD-2つの疾患の密接な関連 (2012)
  • Author(s): 古市賢吾,和田隆志
  • Journal Title: 内科 Volume: 110 Pages: 350-356
• [Journal Article] AKIとCKD―2つの疾患の密接な関連 (2012)
  • Author(s): 古市 賢吾，和田 隆志
  • Journal Title: 内科 Volume: 110 Pages: 350-356
• [Journal Article] The role of cytokine in the lupus nephritis (2011)
  • Author(s): Iwata Y, Furuichi K, Kaneko S, Wada T
  • Journal Title: J Biomed Biotechnol Volume: 2011 Pages: 594809-594809
  • NAID: 120003802994
  • Peer Reviewed
• [Journal Article] Matrix metalloproteinase-2(MMP-2) and membrane-type 1 MMP (MT1-MMP)affect the remodeling of glomerulosclerosis in diabetic (2011)
  • Author(s): Furuichi K, Hisada Y, Shimizu M, Okumura T, Kitagawa K, Yoshimoto K, Iwata Y, Yokoyama H, Kaneko S, Wada T
  • Journal Title: Nephrol Dial Transplant Volume: 0 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Fractalkine and its receptor, CX3CR1, promote hypertensive interstitial fibrosis in the kidney (2011)
  • Author(s): Shimizu K, Furuichi K, Sakai N, Kitagawa K, Matsushima K, Mukaida N, Kaneko S, Wada T
  • Journal Title: Hypertens Res Volume: 34 Pages: 747-752
  • NAID: 10031163247
  • Peer Reviewed
• [Presentation] Effects of adipose-Derived Mesenchymal Cells on Ischemia-Reperfusion Injury in Kidney (2011)
  • Author(s): Kengo Furuichi, Yasuyuki Shinozaki, Shuichi Kaneko, Takashi Wada
  • Organizer: 米国腎臓学会
  • Place of Presentation: ペンシルバニアコンベンションセンター(アメリカ、フィラデルフィア)
  • Year and Date: 2011-11-12
• [Presentation] 腎虚血障害に対する抗サイトカイン、ケモカイン療法 (2010)
  • Author(s): 古市賢吾, 和田隆志
  • Organizer: 日本炎症・再生医学会
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2010-08-06
• [Presentation] 腎虚血障害に対する抗サイトカイン、ケモカイン療法 (2010)
  • Author(s): 古市賢吾, 和田隆志
  • Organizer: 日本炎症・再生医学会
  • Place of Presentation: 京王プ云ザホテル(東京)
  • Year and Date: 2010-08-06
• [Book] KIのすべて (2012)
  • Author(s): 古市賢吾・和田隆志
  • Total Pages: 210
  • Publisher: 南江堂
• [Book] AKIのすべて (2012)
  • Author(s): 古市賢吾・和田隆志（編集）
  • Total Pages: 210
  • Publisher: 南江堂