Search for genes responsible for stress-induced hypertension in SHRSPcongenic rats.

• Project/Area Number: 22590911
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Shimane University
• Principal Investigator: NABIKA Toru 島根大学, 医学部, 教授 (50180534)
• Co-Investigator(Kenkyū-buntansha): HARADA Yuji 島根大学, 医学部, 准教授 (60228658) ; CUI Zh 島根大学, 医学部, 助教 (50379690)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 高血圧 / モデルラット / 遺伝 / 高血圧学 / 遺伝解析

Research Abstract

The stroke-prone spontaneously hypertensive rat (SHRSP) is a genetic model rat for essential hypertension. We previously identified that a region of chromosome 1 harbored a gene (or genes) responsible for exaggerated stress response in SHRSP. In the present study, we narrowed down the region to 1.2 Mbp and found a strong candidate gene that had a truncation in SHRSP.

Research Products

• [Journal Article] Two genomic regions of chromosome 1 and 18 explain most of the stroke susceptibility under salt loading in stroke-prone spontaneously hypertensive rat/Izm. (2013)
  • Author(s): Gandolgor TA, Cui ZH, Nabika T(他 7名)
  • Journal Title: Hypertension
  • Peer Reviewed
• [Journal Article] Standards and pitfalls of focal ischemia models in spontaneously hypertensive rats: With a systematic review of recent articles. (2012)
  • Author(s): Yao H, Nabika T.
  • Journal Title: J Transl Med Volume: 6(10) Issue: 1 Pages: 139-139
  • DOI: 10.1186/1479-5876-10-139
  • Peer Reviewed
• [Journal Article] The stroke-prone spontaneously hypertensive rat: Still a useful model for post-GWAS genetic studies? (2012)
  • Author(s): Nabika T
  • Journal Title: Hypertens Res Volume: 35 Issue: 5 Pages: 477
  • DOI: 10.1038/hr.2012.30
  • NAID: 10030645486
  • Peer Reviewed
• [Journal Article] Effects of hydrogen-rich water on abnormalities in a SHR.Cg -Leprcp/NDmcr rat - a metabolic syndrome rat model. (2011)
  • Author(s): Hashimoto M, Nabika T (他 5名)
  • Journal Title: Med Gas Res Volume: 3(1)1 Issue: 1 Pages: 26-26
  • DOI: 10.1186/2045-9912-1-26
  • Peer Reviewed
• [Journal Article] A 1.8-Mbp fragment on chromosome 1 affects sympathetic response to stress: evaluation in reciprocal congenic strains between stroke-prone spontaneously hypertensive rat and Wistar-Kyoto rat. (2011)
  • Author(s): Xiao B, Harada Y, Kawakami K, Nabika T.
  • Journal Title: J Hypertens Volume: 29 Issue: 2 Pages: 257-65
  • DOI: 10.1097/hjh.0b013e32834137cd
  • Peer Reviewed
• [Journal Article] Characterizing photothrombotic distal middle cerebral artery occlusion and YAG laser-induced reperfusion model in the Izumo strain of spontaneously hypertensive rats. (2011)
  • Author(s): Yao H, Nabika T.
  • Journal Title: Cell Mol Neurobiol Volume: 31 Issue: 1 Pages: 57-63
  • DOI: 10.1007/s10571-010-9553-5
  • Peer Reviewed
• [Journal Article] Characterizing Photothrombotic Distal Middle Cerebral Artery Occlusion and YAG Laser-Induced Reperfusion Model in the Izumo Strain of Spontaneously Hypertensive Rats. (2011)
  • Author(s): Yao H, Nabika T
  • Journal Title: Cellular and molecular neurobiology Volume: 31 Pages: 57-63
  • Peer Reviewed
• [Journal Article] Blood pressure-independent factors determine the susceptibility to delayed neuronal death in the stroke-prone spontaneously hypertensive rats. (2010)
  • Author(s): Sakurai-Yamashita Y, Nabika T, Niwa M.
  • Journal Title: Cell Mol Neurobiol Volume: 30 Issue: 2 Pages: 283-7
  • DOI: 10.1007/s10571-009-9450-y
  • Peer Reviewed
• [Journal Article] A 1.8-Mbp fragment on chromosome 1 affects sympathetic response to stress : evaluation in reciprocal congenic strains between stroke-prone spontaneously hypertensive rat and Wistar-Kyoto rat. (2010)
  • Author(s): Xiao B, Harada Y, Kawakami K, Nabika T
  • Journal Title: Journal of hypertension Volume: 29 Pages: 257-265
  • Peer Reviewed
• [Journal Article] Blood pressure-independent factors determine the susceptibility to delayed neuronal death in the stroke-prone spontaneously hypertensive rats. (2010)
  • Author(s): Sakurai-Yamashita Y, Nabika T, Niwa M.
  • Journal Title: Cellular and molecular neurobiology Volume: 30 Pages: 283-287
  • Peer Reviewed
• [Presentation] 遺伝的モデルラット SHRSP を用いた高血圧,脳卒中遺伝子の探索 (2012)
  • Author(s): 並河 徹
  • Organizer: 第58回日本病理学会秋期特別総会
  • Place of Presentation: ウインクあいち(名古屋市)
  • Year and Date: 2012-11-22
• [Presentation] The prospect for animal research in Japan-Disease models beyond SHR ～ . Cardiovascular Genetics Symposium2012. (2012)
  • Author(s): Nabika T
  • Organizer: 15th International SHR Symposium 48th Japanese SHR SHR Meeting
  • Place of Presentation: Melbourne Brain Cnter.Melbourne(Australia)
  • Year and Date: 2012-09-27
• [Presentation] Search for the genes responsible for exaggerated sympathetic response to stress in SHRSP: genetic and molecular biological approach using congenic strains. Cardiovascular Genetics Symposium2012 (2012)
  • Author(s): Ohara H, Ferdaus Zubaerul Mohammed, Isomura M, Nabika T
  • Organizer: 15th International SHR Symposium 48th Japanese SHR SHR Meeting
  • Place of Presentation: Melbourne Brain Cnter. Melbourne(Australia)
  • Year and Date: 2012-09-27
• [Presentation] 食塩前負荷により中大脳動脈閉塞による脳梗塞は血圧変動を伴わずに増大する:高血圧自然発症ラットとダブルコンジェニックラットを用いた検討 (2012)
  • Author(s): 八尾 博史, 並河 徹
  • Organizer: 第35回日本高血圧学会総会
  • Place of Presentation: ウェスティンナゴヤキャッスル(名古屋市)
  • Year and Date: 2012-09-22
• [Presentation] SHRSP の遺伝的脳卒中感受性の殆どは2つの染色体領域で決定される (2012)
  • Author(s): 並河 徹, 大原 浩貴
  • Organizer: 第35回日本高血圧学会総会
  • Place of Presentation: ウェスティンナゴヤキャッスル(名古屋市)
  • Year and Date: 2012-09-20
• [Presentation] ストレス感受性から見た SHRSP における高血圧原因遺伝子の探索 (2012)
  • Author(s): 大原浩貴,並河 徹
  • Organizer: 第35回日本高血圧学会総会
  • Place of Presentation: ウェスティンナゴヤキャッスル (名古屋市)
  • Year and Date: 2012-09-20
• [Presentation] Further Refinement of the Quantitative Trait Locus Regulating Symapathetic Response in the Stroke-Prone Spontaneously Hypertensive Rat (2012)
  • Author(s): Xiao B, Ohara H, Zubaerul F, Kawakami K, Isomura M, Nabika T
  • Organizer: 22nd European Meeting on Hyertension and cardiovascular Protection
  • Place of Presentation: ExCeL London. London(England)
  • Year and Date: 2012-04-27
• [Presentation] Further Refinement of the Quantitative Trait Locus Regulating Symapathetic Response in the Stroke-Prone Spontaneously Hypertensive Rat. (2012)
  • Author(s): Xiao B
  • Organizer: 22nd European Meeting on Hyertension and cardiovascular Protection.
  • Place of Presentation: ロンドン（イギリス）
• [Presentation] The prospect for animal research in Japan-Disease models beyond SHR～ (2012)
  • Author(s): Nabika T
  • Organizer: Cardiovascular Genetics Symposium2012. 15th International SHR Symposium 48th Japanese SHR SHR Meeting.
  • Place of Presentation: メルボルン（オーストラリア）
• [Presentation] Search for the genes responsible for exaggerated sympathetic response to stress in SHRSP: genetic and molecular biological approach using congenic strains. (2012)
  • Author(s): Ohara H
  • Organizer: Cardiovascular Genetics Symposium2012. 15th International SHR Symposium 48th Japanese SHR SHR Meeting
  • Place of Presentation: メルボルン（オーストラリア）
• [Presentation] SHRSPの遺伝的脳卒中感受性の殆どは２つの染色体領域で決定される (2012)
  • Author(s): 並河 徹
  • Organizer: 第35回日本高血圧学会総会
  • Place of Presentation: ウェスティンナゴヤキャッスル（名古屋市）
• [Presentation] ストレス感受性から見たSHRSPにおける高血圧原因遺伝子の探索 (2012)
  • Author(s): 大原浩貴
  • Organizer: 第35回日本高血圧学会総会
  • Place of Presentation: ウェスティンナゴヤキャッスル（名古屋市）
• [Presentation] 食塩前負荷により中大脳動脈閉塞による脳梗塞は血圧変動を伴わずに増大する：高血圧自然発症ラットとダブルコンジェニックラットを用いた検討 (2012)
  • Author(s): 八尾 博史
  • Organizer: 第35回日本高血圧学会総会
  • Place of Presentation: ウェスティンナゴヤキャッスル（名古屋市）
• [Presentation] 遺伝的モデルラットSHRSPを用いた高血圧，脳卒中遺伝子の探索 (2012)
  • Author(s): 並河 徹
  • Organizer: 第58回日本病理学会秋期特別総会
  • Place of Presentation: ウィンクあいち（名古屋市）
• [Presentation] SHRSP の脳卒中感受性は 2 つの染色体領域に起因する:doublecongenic 系統での検討 (2011)
  • Author(s): 大原浩貴,並河 徹
  • Organizer: 第34回日本高血圧学会総会
  • Place of Presentation: 栃木県総合文化センター(宇都宮市)
  • Year and Date: 2011-10-20
• [Presentation] SHRSPの脳卒中感受性は2つの染色体領域に起因する:double congenic系統での検討 (2011)
  • Author(s): 大原浩貴, 並河徹
  • Organizer: 第34回日本高血圧学会総会
  • Place of Presentation: 栃木県総合文化センター(宇都宮市)
  • Year and Date: 2011-10-20
• [Presentation] 第1,18 染色体上の QTL はSHRSP の脳卒中感受性の大部分を説明する (2011)
  • Author(s): 小川淑子,ツェンデアユーシ ガンドルゴル,大原浩貴,島田大輝,川上浩平, 崔 宗虎,並河 徹
  • Organizer: 第47回高血圧関連疾患モデル学会学術総会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-09-07
• [Presentation] Further refinement of the QTL regulating sympathetic stress-response in SHRSP (2011)
  • Author(s): Ferdaus MZ, Xiao B, Ohara H, Kawakami K, Nabika T
  • Organizer: 第47回高血圧関連疾患モデル学会総会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-09-07
• [Presentation] 第1,18染色体上のQTLはSHRSPの脳卒中感受性の大部分を説明する (2011)
  • Author(s): 小川淑子, 並河徹, 他5名
  • Organizer: 第47回高血圧関連疾患モデル学会学術総会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-09-07
• [Presentation] 遺伝解析に基づく新たなモデルラット作成: congenic strategy からの展開 (2011)
  • Author(s): 並河 徹 , 崔 宗虎 ,小川淑子,Tsend-Ayush Gandolgor, 島田大輝,川上浩平,大原浩貴,磯村 実
  • Organizer: 第47回高血圧関連疾患モデル学会学術総会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-09-06
• [Presentation] 遺伝解析に基づく新たなモデルラット作成:congenic strategyからの展開 (2011)
  • Author(s): 並河徹, 他7名
  • Organizer: 第47回高血圧関連疾患モデル学会学術総会
  • Place of Presentation: 北海道大学(札幌市)(招待講演)
  • Year and Date: 2011-09-06
• [Presentation] Synergic Efefect of Two Qtls onchromosome 1 and 18 Accounts for the Difference of StrokeSusceptibility Between SHR and SHRSP; A Sutudy on Double-Congenic Strains (2011)
  • Author(s): Nabika T, Cui ZH, Ogawa T, Gandolgor TA, Kawakami K, Ohara H
  • Organizer: 21st European Meeting on Hypertension and Cardiovascular Prevention
  • Place of Presentation: MICO Milano Congressi. Milano(Italy)
  • Year and Date: 2011-06-18
• [Presentation] SHRSP由来の第1、18 染色体領域は相乗的に SHR の脳卒中感受性を亢進させる (2011)
  • Author(s): ツェンデアユーシ ガンドルゴル,小川淑子,大原浩貴, 崔 宗虎, 並河 徹
  • Organizer: 第58回日本実験動物学会総会
  • Place of Presentation: タワーホール船堀(東京都江戸川区)
  • Year and Date: 2011-05-26
• [Presentation] SHRSP由来の第1、18染色体領域は相乗的にSHRの脳卒中感受性を亢進させる (2011)
  • Author(s): ツェンデアユーシ ガンドルゴル, 並河徹, 他3名
  • Organizer: 第58回日本実験動物学会総会
  • Place of Presentation: タワーホール船掘(東京都)
  • Year and Date: 2011-05-26
• [Presentation] Effect of a 1.8-Mbp genomic fragment on chromosome 1 on sympathetic response to stressors : Evalution in reciprocal congenic strains between SHRSP and WKY. (2010)
  • Author(s): Xiao B, Nabika T
  • Organizer: The X VIIIth International Workshop on Genetic Systems in the Rat.
  • Place of Presentation: Kyoto
  • Year and Date: 2010-12-02
• [Presentation] Effect of a 1.8-Mbp genomic fragment on chromosome 1 on sympathetic response to stressors: Evalution in reciprocal congenic strains between SHRSP and WKY (2010)
  • Author(s): Xiao B, Nabika T
  • Organizer: The X VIII th International Workshop on Genetic Systems in the Rat
  • Place of Presentation: 京都大学(京都市)
  • Year and Date: 2010-12-01
• [Presentation] Synergic effect of two QTLs on chromosome 1 and 18 accounts for the difference of stroke-susceptibility between SHR and SHRSP; A study on a double-congenic strain (2010)
  • Author(s): Cui ZH, Ogawa T, Gandolgor T-A, Xiao B, Kawakami K, Nabika T
  • Organizer: The XVIII th International Workshop on Genetic Systems in the Rat
  • Place of Presentation: 京都大学(京都市)
  • Year and Date: 2010-12-01
• [Presentation] Synergic effect of two QTLs on chromosome 1 and 18 accounts for the difference of stroke-susceptibility between SHR and SHRSP ; A study on a double-congenic strain. (2010)
  • Author(s): Cui ZH, Ozawa T, Gandolgor T-A, Xiao B, Kawakami K, Nabika T
  • Organizer: The X VIIIth International Workshop on Genetic Systems in the Rat.
  • Place of Presentation: Kyoto
  • Year and Date: 2010-12-01
• [Presentation] ラット第1 染色体上の 1.5Mbpの領域が SHRSP のストレス反応性に関与する (2010)
  • Author(s): 並河 徹
  • Organizer: 第33回日本高血圧学会総会
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2010-10-16
• [Presentation] Effect of a small genomic fragment on chromosome 1 on sympathetic response to stressors: Evaluation in reciprocal congenic strains between SHRSP and WKY (2010)
  • Author(s): Xiao B, Nabika T
  • Organizer: 6th International ISP Congress and 14th International SHR Sympsium
  • Place of Presentation: Palais des Congres. Montreal(Canada)
  • Year and Date: 2010-09-23
• [Presentation] Effect of a small genomic fragment on chromosome 1 on sympathetic response to stressors : Evaluation in reciprocal congenic strains between SHRSP and WKY. (2010)
  • Author(s): Xiao B, Nabika T
  • Organizer: 6^<th> International ISP Congress and 14^<th> International SHR Sympsium.
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2010-09-23
• [Presentation] Association of the plasma ARG/ADMA ratio with the salt-sensitivity (2010)
  • Author(s): Nabika T, Yasui N, Notsu Y, Njelekela M, Nara Y, Yamori Y, Ikeda K
  • Organizer: 20th European Meeting on Hypertension
  • Place of Presentation: Norway Exhibition & Convention Center. Oslo(Norway)
  • Year and Date: 2010-06-20
• [Presentation] Effects of small genomic fagment on chromosome 1 on the sympathetic reavtivity to stresses: Evaluation in the reciprocal cogenic strains between WKY and SHRSP (2010)
  • Author(s): Xiao B, Nabika T
  • Organizer: 第57回日本実験動物学会総会
  • Place of Presentation: 京都テルサ(京都市)
  • Year and Date: 2010-05-13
• [Presentation] SHRSP/Izm とSHR/Izm のコンジェニックラットによる脳卒中関連遺伝子の探索
  • Author(s): 崔 宗虎 , 並河 徹
  • Organizer: 第57回日本実験動物学会総会
  • Place of Presentation: 京都テルサ (京都市)
• [Book] Standards and pitfalls regarding the SHR model in genetic hypertension and stroke. In: Hypertension, Heredity and Stroke eds (2010)
  • Author(s): Yao H, Nabika T
  • Publisher: Moratinos C, Cardenas B, Nova Science
• [Book] 循環器疾患 (2010)
  • Author(s): 並河 徹
  • Publisher: 株式会社エル・アイ・シー