Expression of MAK-V/Hunk in renal distal tubules and its possible involvement in proliferative suppression

• Project/Area Number: 22590913
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Yokohama City University
• Principal Investigator: KANAOKA Tomohiko 横浜市立大学, 附属病院, 指導診療医 (70551258)
• Co-Investigator(Kenkyū-buntansha): TAMURA Kouichi 横浜市立大学, 医学部, 准教授 (40285143)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 遺伝子 / 細胞・組織 / 発生・文化 / 循環器・高血圧 / シグナル伝達 / 発生・分化 / 腎臓・高血圧 / 生体分子 / 生理活性

Research Abstract

We demonstrated that high-salt loading suppressed renal MAK-V/Hunk mRNA levels in wild-type mice. To analyze the possible renal function of HUNK/MAK-V, we generated MAK-V/Hunk transgenic mice. The MAK-V/Hunk transgenic mice exhibited the suppression of high salt loading-mediated hypertension compared with wild-type mice. We also showed an altered renal distribution of HUNK/MAK-V inthe diseased human kidney. Collectively, these results indicate a critical role of renal MAK-V/Hunk in the renal sodium handling and blood pressure regulation.

Research Products

• [Journal Article] Upstream stimulatory factors 1 and 2 mediate the transcription of angiotensin II binding and inhibitory protein (2013)
  • Author(s): Matsuda M, Tamura K, Wakui H, Maeda A, Ohsawa M, Kanaoka T, Azushima K, Uneda K, Haku S, Tsurumi-Ikeya Y, Toya Y, Maeshima Y,Yamashita A, Umemura S
  • Journal Title: J Biol Chem Volume: (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Therapeutic potential of low-density lipoprotein apheresis in the management of peripheral artery disease in patients with chronic kidney disease (2013)
  • Author(s): Tamura K, Tsurumi-Ikeya Y, Wakui H, Maeda A, Ohsawa M, Azushima K, Kanaoka T, Uneda K, Haku S, Azuma K, Mitsuhashi H, Tamura N, Toya Y, Tokita Y, Kokuho T, Umemura S
  • Journal Title: Ther Apher Dial Volume: 17(2) Pages: 185-192
  • Peer Reviewed
• [Journal Article] Enhanced Ang receptor-associated protein in renal tubule suppressesAng-dependent hypertension (2013)
  • Author(s): Wakui H, Tamura K, Masuda S, Tsurumi-Ikeya Y, Fujita M, Maeda A, Ohsawa M, Azushima K, Uneda K, Matsuda M, Kitamura K, Uchida S, Toya Y, Kobori H, Nagahama K, Yamashita A, Umemura S
  • Journal Title: Hypertension Volume: 61(6) Pages: 1203-1210
  • Peer Reviewed
• [Journal Article] The physiology and pathophysiology of a novel angiotensin receptor-binding protein ATRAP/Agtrap (2013)
  • Author(s): Tamura K, Wakui H, Maeda A, Dejima T, Ohsawa M, Azushima K, Kanaoka T, Haku S, Uneda K, Masuda S, Azuma K, Shigenaga A, Koide Y, Tsurumi-Ikeya Y, Matsuda M, Toya Y, Tokita Y, YamashitaA, Umemura S
  • Journal Title: Curr Pharm Des Volume: 19(17) Pages: 3043-3048
  • Peer Reviewed
• [Journal Article] The angiotensin II type 1 receptor blocker olmesartan preferentially improves nocturnal hypertension and proteinuria in chronic kidney disease (2013)
  • Author(s): Yanagi M, Tamura K, Fujikawa T, Wakui H, Kanaoka T, Ohsawa M, Azushima K, Maeda A, Kobori H, Umemura S.
  • Journal Title: Hypertens Res Volume: 36(3) Pages: 262-269
  • Peer Reviewed
• [Journal Article] UPSTREAM STIMULATORY FACTORS 1 AND 2 MEDIATE THE TRANSCRIPTION OF ANGIOTENSIN II BINDING AND INHIBITORY PROTEIN (2013)
  • Author(s): Matsuda M, Kanaoka T, et al.
  • Journal Title: Journal of Biological Chemistry Volume: -
  • Peer Reviewed
• [Journal Article] The physiology and pathophysiology of a novel angiotensin receptor-binding protein ATRAP/Agtrap (2013)
  • Author(s): Tamura K, Kanaoka T, et al.
  • Journal Title: Current Pharmaceutical Design Volume: -
  • Peer Reviewed
• [Journal Article] Angiotensin II Type 1 Receptor Blocker Olmesartan Preferentially Improves Nocturnal Hypertension and Proteinuria in Chronic Kidney Disease (2013)
  • Author(s): Yanagi M, Kanaoka T, et al.
  • Journal Title: Hypertension Research Volume: -
  • Peer Reviewed
• [Journal Article] Effects of aliskiren-based therapy on ambulatory blood pressure profile, central hemodynamics, and arterial stiffness in nondiabetic mild to moderate hypertensive patients (2012)
  • Author(s): Kanaoka T, Tamura K, Ohsawa M, Wakui H, Maeda A, Dejima T, Azushima K, Haku S, Mitsuhashi H, Yanagi M, Oshikawa J, Uneda K, Aoki K, Fujikawa T, Toya Y, Uchino K, Umemura S
  • Journal Title: J Clin Hypertens (Greenwich) Volume: 14(8) Pages: 522-529
  • Peer Reviewed
• [Journal Article] Relationship of ambulatory blood pressure and the heart rate profile with renal function parameters in hypertensive patients with chronic kidney disease (2012)
  • Author(s): Kanaoka T, Tamura K, Ohsawa M, Yanagi M, Haku S, Wakui H, Maeda A, Dejima T, Azushima K, Mitsuhashi H, Okano Y, Fujikawa T, Toya Y, Mizushima S, Tochikubo O, Umemura S
  • Journal Title: Clin Exp Hypertens Volume: 34(4) Pages: 264-269
  • Peer Reviewed
• [Journal Article] Combination therapy of angiotensin II receptor blocker and calcium channel blocker exerts pleiotropic therapeutic effects in addition to blood pressure lowering:amlodipine and candesartan trial in Yokohama (ACTY) (2012)
  • Author(s): Maeda A, Tamura K, Kanaoka T, Ohsawa M, Haku S, Azushima K, Dejima T, Wakui H, Yanagi M, Okano Y, Fujikawa T, Toya Y, Mizushima S, Tochikubo O, Umemura S
  • Journal Title: Clin Exp Hypertens. Volume: 34(4) Pages: 249-257
  • Peer Reviewed
• [Journal Article] Agonist-independent constitutive activity of angiotensin II receptor promotes cardiac remodeling in mice (2012)
  • Author(s): Yasuda N, Akazawa H, Ito K, Shimizu I, Kudo-Sakamoto Y, Yabumoto C, Yano M, Yamamoto R, Ozasa Y, Minamino T, Naito A, Oka T, Shiojima I, Tamura K, Umemura S, Paradis P, Nemer M, Komuro I
  • Journal Title: Hypertension Volume: 59 (3) Pages: 627-633
  • Peer Reviewed
• [Journal Article] Effects of Aliskiren-Based Therapy on Ambulatory Blood Pressure Profile, Central Hemodynamics, and Arterial Stiffness in Nondiabetic Mild to Moderate Hypertensive Patients (2012)
  • Author(s): Kanaoka T, Tamura K, et al.
  • Journal Title: Journal of Clinical Hypertension Volume: 14 Issue: 8 Pages: 522-529
  • DOI: 10.1111/j.1751-7176.2012.00640.x
  • Peer Reviewed
• [Journal Article] Relationship of Ambulatory Blood Pressure and the Heart Rate Profile with Renal Function Parameters in Hypertensive Patients with Chronic Kidney Disease (2012)
  • Author(s): Kanaoka T, Tamura K, et al.
  • Journal Title: Clinical and Experimental Hypertension Volume: 34 Issue: 4 Pages: 264-269
  • DOI: 10.3109/10641963.2012.681082
  • Peer Reviewed
• [Journal Article] Emerging concept of anti-hypertensive therapy based on ambulatory blood pressure profile in chronic kidney disease (2011)
  • Author(s): Tamura K, Kanaoka T, Ohsawa M, Haku S, Azushima K, Maeda A, Dejima T, Wakui H, Ozawa M, Shigenaga A, Umemura S
  • Journal Title: Am J Cardiovasc Dis Volume: 1 (3) Pages: 236-243
  • Peer Reviewed
• [Journal Article] Prepubertal angiotensin blockade exerts long-term therapeutic effect through sustained ATRAP activation in salt-sensitive hypertensive rats (2011)
  • Author(s): Dejima T, Tamura K, Wakui H, Maeda A, Ohsawa M, Kanaoka T, Haku S, Azushima K, Masuda S, Shigenaga A, Azuma K, Matsuda M, Yabana M, Hirose T, Uchino K, Kimura K, Nagashima Y, Umemura S
  • Journal Title: J Hypertens Volume: 29 (10) Pages: 1919-1929
  • Peer Reviewed
• [Journal Article] Effects of multiple factorial intervention on ambulatory BP profile and renal function in hypertensive type 2 diabetic patients with overt nephropathy -A pilot study (2011)
  • Author(s): Kanaoka T, Tamura K, Moriya T, Tanaka K, Koono Y, Kondoh S, Toyoda M, Umezono T, Fujikawa T, Ohsawa M, Dejima T, Maeda A, Wakui H, Haku S, Yanagi M, Mitsuhashi H, Ozawa M, Okano Y, Ogawa N, Yamakawa T, Mizushima S, Suzuki D, Umemura S
  • Journal Title: Clin Exp Hypertens Volume: 33 (4) Pages: 255-263
  • Peer Reviewed
• [Journal Article] Involvement of Runx3 in the basal transcriptional activation of the mouse angiotensin II type 1receptor-associated protein gene (2011)
  • Author(s): Matsuda M, Tamura K, Wakui H, Dejima T, Maeda A, Ohsawa M, Kanaoka T, Haku S, Azushima K, Yamasaki H, Saito D, Hirose T, Maeshima Y, Nagashima Y, Umemura S
  • Journal Title: Physiol Genomics Volume: 43 (14) Pages: 884-894
  • Peer Reviewed
• [Journal Article] Effects of multiple factorial intervention on ambulatory BP profile and renal function in hypertensive type 2 diabetic patients with overt nephropathy-A pilot study (2011)
  • Author(s): Kanaoka T, et al
  • Journal Title: Clin Exp Hypertens Volume: 33 Issue: 4 Pages: 255-263
  • DOI: 10.3109/10641963.2011.583971
  • Peer Reviewed
• [Journal Article] Involvement of Runx3 in the basal transcriptional activation of the mouse angiotensin II type 1 receptor-associated protein gene (2011)
  • Author(s): Matsuda M, Kanaoka T, et al
  • Journal Title: Physiol Genomics Volume: 43 Issue: 14 Pages: 884-894
  • DOI: 10.1152/physiolgenomics.00005.2011
  • Peer Reviewed
• [Journal Article] Prepubertal angiotensin blockade exerts long-term therapeutic effect through sustained ATRAP activation in salt-sensitive hypertensive rats (2011)
  • Author(s): Dejima T, Kanaoka T, et al
  • Journal Title: J Hypertens Volume: 29 Issue: 10 Pages: 1919-1929
  • DOI: 10.1097/hjh.0b013e32834a5a46
  • Peer Reviewed
• [Journal Article] Intrarenal suppression of angiotensin II type 1 receptor binding molecule in angiotensin II-infused mice (2010)
  • Author(s): Wakui H, Tamura K, Matsuda M, Bai Y, Dejima T, Shigenaga A, Masuda S, Azuma K, Maeda A, Hirose T, Ishigami T, Toya Y, Yabana M, Minamisawa S, Umemura S
  • Journal Title: Am J Physiol Renal Physiol Volume: 299(5)
  • Peer Reviewed
• [Journal Article] Expression of angiotensin II type 1 receptor-interacting molecule in normal human kidney and IgA nephropathy (2010)
  • Author(s): Masuda S, Tamura K, Wakui H, Maeda A, Dejima T, Hirose T, Toyoda M, Azuma K, Ohsawa M, Kanaoka T, Yanagi M, Yoshida S, Mitsuhashi H, Matsuda M, Ishigami T, Toya Y, Suzuki D, Nagashima Y, Umemura S
  • Journal Title: Am J Physiol Renal Physiol Volume: 299(4)
  • Peer Reviewed
• [Journal Article] Expression of angiotensin II type 1 receptor-interacting molecule in normal human kidney and IgA nephropathy. (2010)
  • Author(s): Masuda S, Kanaoka T, et al.
  • Journal Title: Am J Physiol Renal Physiol. Volume: 299
  • Peer Reviewed
• [Presentation] Ambulatory Blood Pressure and Heart Rate Profile with Renal Function Parameters in Hypertensive Patients with Chronic Kidney Disease (2013)
  • Author(s): Kanaoka T, Tamura K, et al.
  • Organizer: 第23回欧州高血圧学会 The 23rd European Meeting on Hypertension and Cardiovascular Prevention
  • Place of Presentation: Milan, Italy
• [Presentation] Relationship of ambulatory blood pressure and heart rate profile with renal function parameters in hypertensive patients with chronic kidney disease (2012)
  • Author(s): Kanaoka T, Tamura K, et al
  • Organizer: 第22回欧州高血圧学会The 22nd European Meeting on Hypertension and Cardiovascular protection
  • Place of Presentation: London, UK
• [Presentation] Relationship of ambulatory blood pressure and heart rate profile with renal function parameters in hypertensive patients with chronic kidney disease. (2012)
  • Author(s): Kanaoka T, Tamura K, et al.
  • Organizer: 第22回欧州高血圧学会 The 22nd European Meeting on Hypertension and Cardiovascular protection
  • Place of Presentation: London, UK
• [Presentation] 高血圧合併慢性腎臓病患者に対するL型N型カルシウムチャネル拮抗薬とL型カルシウムチャネル拮抗薬の臨床効果についての比較検討 (2012)
  • Author(s): 金岡知彦他．
  • Organizer: 第35回日本高血圧学会総会
  • Place of Presentation: ウエステインナゴヤキャッスル（愛知県）
• [Presentation] 蛋白質リン酸化酵素MAK-V/Hunkは遠位尿細管細胞でのAT1受容体活性化を抑制する (2011)
  • Author(s): 金岡知彦, 他
  • Organizer: 第34回日本高血圧学会総会
  • Place of Presentation: 栃木県総合文化センター(栃木県)
  • Year and Date: 2011-10-20
• [Presentation] Angiotensin II Type 1 Receptor-Associated Protein Mediates a Novel Inhibitory Effect on Cardiac Hypertrophy In Vivo (2010)
  • Author(s): Tamura K, Wakui H, Shigenaga A, Kanaoka T, et al.
  • Organizer: Scientific Sessions 2010, American Heart Association
  • Place of Presentation: McCormick Place-Chicago, IL, USA
  • Year and Date: 2010-11-15
• [Book] CKD診療ガイド2012 Q&A. (2013)
  • Author(s): 田村功一,前田晃延,金岡知彦
  • Total Pages: 181
  • Publisher: 診断と治療社(東京)
• [Book] CKD診療ガイド2012 Q＆A (2013)
  • Author(s): 田村功一，前田晃延，金岡知彦．
  • Total Pages: 181
  • Publisher: 診断と治療社