Investigation of klotho protein on protective role of peritoneal membrane

• Project/Area Number: 22590920
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Kawasaki Medical School
• Principal Investigator: SASAKI Tamaki 川崎医科大学, 医学部, 教授 (30187124)
• Co-Investigator(Kenkyū-buntansha): SATOH Minoru 川崎医科大学, 医学部, 講師 (70449891) ; FUJIMOTO Sohachi 川崎医科大学, 医学部, 講師 (00319948)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 人工透析学 / Klotho蛋白 / 腹膜線維化 / 腹膜中皮細胞 / Wnt-signal系 / 形質変換 / WNTシグナル / klotho蛋白

Research Abstract

The one of obstacles to successful long-term PD is peritoneal fibrosis in peritoneal membrane after exposure to non-physiologic dialysis solutions. Studies have demonstrated that peritoneal mesothelial cells undergoepithelial-to-mesenchymal transition (EMT) after expose to injury. EMT is the important role of the initial peritoneal fibrosis. The induction of EMT is associated with activation of Wnt-dependent beta-catenin signaling and the secreted klotho protein can regulate Wnt signaling. Overexpression of Klotho protein protects the peritoneal membranethrough attenuation of Wnt-signaling.

Research Products

• [Journal Article] Selective estrogen receptor modulation attenuates proteinuria-induced renal tubular damage by modulating mitochondrial oxidative status (2013)
  • Author(s): Nishi Y, Satoh M, Nagasu H, Kadoya H, Ihoriya C, Kidokoro K, Sasaki T, Kashihara N
  • Journal Title: Kidney Int Volume: 83(4) Issue: 4 Pages: 662-73
  • DOI: 10.1038/ki.2012.475
  • Peer Reviewed
• [Journal Article] Klotho protects against mouse renal fibrosis by inhibiting Wnt signaling (2012)
  • Author(s): Satoh M, Nagasu H, Morita Y, Yamaguchi TP, Kanwar YS, Kashihara N
  • Journal Title: Am JPhysiol Renal Physiol Volume: 303(12) Issue: 12 Pages: F1641-F1651
  • DOI: 10.1152/ajprenal.00460.2012
  • Peer Reviewed
• [Journal Article] Klotho蛋白による腹膜機能保護作用のメカニズム解析. (2012)
  • Author(s): 佐々木環.
  • Journal Title: 川崎医学会誌 Volume: 38(2)
• [Journal Article] Angiostatin production increases in response to decreased nitric oxide in aging rat kidney (2012)
  • Author(s): Satoh M, Kidokoro K, Ozeki M, Nagasu H, Nishi Y, Ihoriya C, Fujimoto S, Sasaki T, Kashihara N
  • Journal Title: Lab Invest Volume: 93(3) Issue: 3 Pages: 334-43
  • DOI: 10.1038/labinvest.2012.171
  • Peer Reviewed
• [Journal Article] Establishment of a blood purification system for renal failure rats usingsmall-size dialyzer membranes. (2012)
  • Author(s): Yorimitsu D, Satoh M, Koremoto M, Haruna Y, Nagasu H, Kuwabara A, Sasaki T, Kashihara N
  • Journal Title: Ther Apher Dial Volume: 16(6) Issue: 6 Pages: 566-72
  • DOI: 10.1111/j.1744-9987.2012.01091.x
  • Peer Reviewed
• [Journal Article] Tacrolimus Induces Glomerular Injury via Endothelial Dysfunction Caused by Reactive Oxygen Species and Inflammatory Change. (2012)
  • Author(s): Kidokoro K, Satoh M, Nagasu H, Sakuta T, Kuwabara A, Yorimitsu D, Nishi Y, Tomita N, Sasaki T, Kashihara N.
  • Journal Title: Kidney Blood Press Res. Volume: 35(6) Issue: 6 Pages: 549-57
  • DOI: 10.1159/000339494
  • Peer Reviewed
• [Journal Article] Azelnidipine attenuates glomerular damage in Dahl salt-sensitive rats by suppressing sympathetic nerve activity. (2012)
  • Author(s): Nagasu H, Satoh M, Fujimoto S, Tomita N, Sasaki T, Kashihara N
  • Journal Title: Hypertens Res Volume: 35(3) Issue: 3 Pages: 348-55
  • DOI: 10.1038/hr.2011.184
  • NAID: 10030644635
  • Peer Reviewed
• [Journal Article] Endothelial dysfunction promotes the transition from compensatory renal hypertrophy to kidney injury after unilateral nephrectomy in mice (2012)
  • Author(s): Nagasu H
  • Journal Title: Am J Physiol Renal Physiol Volume: (in press) Issue: 11 Pages: F1402-F1408
  • DOI: 10.1152/ajprenal.00459.2011
  • Peer Reviewed
• [Journal Article] Overexpression of klotho protein modulates uninephrectomy-induced compensatory renal hypertrophy bysuppressing IGF-I signals. (2011)
  • Author(s): Nagasu H, Satoh M, Kuwabara A, Yorimitsu D, Kidokoro K, Nishi Y, Tomita N, Sasaki T, Kashihara N.
  • Journal Title: BiochemBiophys Res Commun. Volume: 407(1) Issue: 1 Pages: 39-43
  • DOI: 10.1016/j.bbrc.2011.02.089
  • Peer Reviewed
• [Presentation] Klotho蛋白による腹膜保護作用の検討 (2012)
  • Author(s): 西佑子、佐藤稔、藤本壮八、佐々木環、柏原直樹
  • Organizer: 第57回日本透析医学会学術集会
  • Place of Presentation: 札幌市
  • Year and Date: 2012-06-24
• [Presentation] Klotho蛋白のWntシグナル抑制による抗繊維化効果. (2012)
  • Author(s): 佐藤稔、佐々木環、柏原直樹.
  • Organizer: 第12回日本抗加齢医学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2012-06-24
• [Presentation] Klotho蛋白のWntシグナル抑制による抗繊維化効果 (2012)
  • Author(s): 佐藤稔、佐々木環、柏原直樹
  • Organizer: 第12回日本抗加齢医学会総会
  • Place of Presentation: 横浜市
  • Year and Date: 2012-06-24
• [Presentation] Klotho蛋白導入による腎繊維化抑制効果の検討 (2012)
  • Author(s): 佐藤稔、佐々木環、柏原直樹.
  • Organizer: 第55回日本腎臓学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2012-06-02
• [Presentation] Klotho蛋白によるWntシグナル制御を介した腹膜保護作用の検討 (2012)
  • Author(s): 西佑子、佐藤稔、藤本壮八、佐々木環、柏原直樹
  • Organizer: 第55回日本腎臓学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2012-06-02
• [Presentation] Klotho ProteinReduces Mouse Renal Fibrosis after Unilateral Ureteral Obstruction through Inhibition of Wnt Signaling. (2012)
  • Author(s): Satoh M, Nagasu H, Kidokoro K, Nishi Y, Ihoriya C, Kadoya H, Sasaki T, Kashihara N.
  • Organizer: 49th ERA-EDTA Congress
  • Place of Presentation: Paris,France
  • Year and Date: 2012-05-26
• [Presentation] Klotho蛋白の高糖濃度による近医尿細管上皮細胞障害防御機構の解明 (2011)
  • Author(s): 春名克祐、佐藤稔、長洲一、城所研吾、西佑子、庵谷千恵子、冨田奈留也、佐々木環、柏原直樹.
  • Organizer: 第54回日本腎臓学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-06-15
• [Presentation] Klotho Attenuates Inflammation of Adipose Tissue and Development of Insulin Resistance in High-Fat Induced Obese Mice. (2010)
  • Author(s): Satoh M, Kidokoro K, Nagasu H, Ihoriya C, Nishi Y, Kadoya H, Sasaki T, Kashihara N.
  • Organizer: Kidney Week 2012
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-11-02
• [Presentation] 演題 Klotho蛋白によるWntシグナル制御を介した 腹膜保護作用の検討
  • Author(s): 西 佑子
  • Organizer: 第55回腎臓学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] Klotho蛋白による腹膜保護作用の検討
  • Author(s): 西 佑子
  • Organizer: 第57回日本透析医学会学術集会
  • Place of Presentation: 札幌プリンスホテル国際パミール（札幌）
• [Remarks]
  • URL: http://www.kawasaki-jinzo.net/